H3C

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$40.00
SKU: H3C
View product citations for antibody H3C on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: synuclein, alpha & beta
Hybridoma Cells Available: Yes
Antigen Species: Canary
Depositor: George, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Illinois
Positive Tested Species Reactivity: Avian, Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 15 kDa; Apparent: 19 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SNCA
Immunogen: C-terminal of Canary synuclein cojugated to KLH
Alternate Gene Names: synelfin, NACP
Alternate Antibody Name: H3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: C-terminal (C-EMPPEEEYQDYEPEA)
Uniprot ID: Q91448 
Immunogen Sequence: peptide (C-EMPPEEEYQDYEPEA)
Entrez Gene ID: 103826045 
Additional Characterization:  
Antibody Registry ID: AB_2618046 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H3C was deposited to the DSHB by George, J. (DSHB Hybridoma Product H3C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Characterization of a novel protein regulated during the critical period for song learning in the zebra finch.
    Clayton DF
    Neuron 15.2 (1995 Aug): 361-72.

    Delayed localization of synelfin (synuclein, NACP) to presynaptic terminals in cultured rat hippocampal neurons.
    Clayton DF
    Brain research. Developmental brain research 99.1 (1997 Mar 17): 87-94.

    Protein-protein interactions of alpha-synuclein in brain homogenates and transfected cells.
    George JM
    Brain research. Molecular brain research 95.1-2 (2001 Nov 1): 138-45.

    Exposure to long chain polyunsaturated fatty acids triggers rapid multimerization of synucleins.
    George JM
    The Journal of biological chemistry 276.45 (2001 Nov 9): 41958-62.

    Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines.
    George JM
    Neuroscience letters 349.2 (2003 Oct 2): 133-5.

    Mapping the subcellular distribution of α-synuclein in neurons using genetically encoded probes for correlated light and electron microscopy: implications for Parkinson's disease pathogenesis.
    Ellisman MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.6 (2013 Feb 6): 2605-15.

    Alpha-synuclein aggregation involves a bafilomycin A 1-sensitive autophagy pathway.
    Winkler J
    Autophagy 8.5 (2012 May 1): 754-66.

    Alpha-synuclein-enhanced green fluorescent protein fusion proteins form proteasome sensitive inclusions in primary neurons.
    Hyman BT
    Neuroscience 104.3 (2001): 901-12.

    Stabilization of alpha-synuclein secondary structure upon binding to synthetic membranes.
    George JM
    The Journal of biological chemistry 273.16 (1998 Apr 17): 9443-9.

    IP References

    Protein-protein interactions of alpha-synuclein in brain homogenates and transfected cells.
    George JM
    Brain research. Molecular brain research 95.1-2 (2001 Nov 1): 138-45.

    Epitope Map References
    All References

    Characterization of a novel protein regulated during the critical period for song learning in the zebra finch.
    Clayton DF
    Neuron 15.2 (1995 Aug): 361-72.

    Delayed localization of synelfin (synuclein, NACP) to presynaptic terminals in cultured rat hippocampal neurons.
    Clayton DF
    Brain research. Developmental brain research 99.1 (1997 Mar 17): 87-94.

    A close association of torsinA and alpha-synuclein in Lewy bodies: a fluorescence resonance energy transfer study.
    Hyman BT
    The American journal of pathology 159.1 (2001 Jul): 339-44.

    Design, Synthesis, and Characterization of 3-(Benzylidene)indolin-2-one Derivatives as Ligands for α-Synuclein Fibrils.
    Mach RH
    Journal of medicinal chemistry 58.15 (2015 Aug 13): 6002-17.

    Alpha-synuclein aggregation involves a bafilomycin A 1-sensitive autophagy pathway.
    Winkler J
    Autophagy 8.5 (2012 May 1): 754-66.

    Development and screening of contrast agents for in vivo imaging of Parkinson's disease.
    Bacskai BJ
    Molecular imaging and biology 15.5 (2013 Oct): 585-95.

    Alpha-synuclein-enhanced green fluorescent protein fusion proteins form proteasome sensitive inclusions in primary neurons.
    Hyman BT
    Neuroscience 104.3 (2001): 901-12.

    alpha-Synuclein immunoreactivity in dementia with Lewy bodies: morphological staging and comparison with ubiquitin immunostaining.
    Hyman BT
    Acta neuropathologica 99.4 (2000 Apr): 352-7.

    α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton.
    Feany MB
    Neuron 97.1 (2018 Jan 3): 108-124.e6.

    Protein-protein interactions of alpha-synuclein in brain homogenates and transfected cells.
    George JM
    Brain research. Molecular brain research 95.1-2 (2001 Nov 1): 138-45.

    Exposure to long chain polyunsaturated fatty acids triggers rapid multimerization of synucleins.
    George JM
    The Journal of biological chemistry 276.45 (2001 Nov 9): 41958-62.

    Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines.
    George JM
    Neuroscience letters 349.2 (2003 Oct 2): 133-5.

    Mapping the subcellular distribution of α-synuclein in neurons using genetically encoded probes for correlated light and electron microscopy: implications for Parkinson's disease pathogenesis.
    Ellisman MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.6 (2013 Feb 6): 2605-15.

    Stabilization of alpha-synuclein secondary structure upon binding to synthetic membranes.
    George JM
    The Journal of biological chemistry 273.16 (1998 Apr 17): 9443-9.

    Fiber diffraction of synthetic alpha-synuclein filaments shows amyloid-like cross-beta conformation.
    Crowther RA
    Proceedings of the National Academy of Sciences of the United States of America 97.9 (2000 Apr 25): 4897-902.

    Accelerated in vitro fibril formation by a mutant alpha-synuclein linked to early-onset Parkinson disease.
    Lansbury PT
    Nature medicine 4.11 (1998 Nov): 1318-20.

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