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12G10 anti-alpha-tubulin

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$40.00
SKU: 12G10 anti-alpha-tubulin
View product citations for antibody 12G10 anti-alpha-tubulin on CiteAb

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Available: 200

DSHB Data Sheet

Catalog Fields

Antigen: tubulin (alpha-)
Hybridoma Cells Available: Yes
Antigen Species: Tetrahymena
Depositor: Frankel, J. / Nelsen, E.M.
Isotype: MIgG1
Antigen Sequence: 3139885 8020112
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Algae, Avian, C. elegans, Cephalopod, Dictyostelium, Drosophila, Fish, Hermit Crab, Human, Medusa, Mouse, Protozoa, Quail, Saccharomyces, Tetrahymena, Toxoplasma gondii, Xenopus
Depositors Notes:
Antigen Molecular Weight: Predicted: 49.59 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ATU1
Immunogen: alpha tubulin, Tetrahymena thermophila and Tetrahymena pyriformis (mixture)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P41351 
Immunogen Sequence: alpha tubulin
Entrez Gene ID: 7838142 
Additional Characterization:
Antibody Registry ID: AB_1157911 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12G10 anti-alpha-tubulin was deposited to the DSHB by Frankel, J. / Nelsen, E.M. (DSHB Hybridoma Product 12G10 anti-alpha-tubulin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

29 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Nature cell biology
    Gaertig J
    Nature cell biology 4.3 (2002 Mar): 256-9.

    IF References

    Eukaryotic cell
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna
    Manolescu N
    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna 39.6 (1987 Nov-Dec): 513-20.

    Parasitology
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    Cell
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    Developmental biology
    Piano F
    Developmental biology 335.1 (2009 Nov 1): 253-62.

    Developmental cell
    Weis WI
    Developmental cell 23.3 (2012 Sep 11): 533-46.

    Journal of morphology
    Page LR
    Journal of morphology 278.1 (2017 Jan): 29-49.

    The Journal of comparative neurology
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    The Journal of comparative neurology
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    The American journal of Chinese medicine
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Journal of cell science
    Pearson CG
    Journal of cell science 132.15 (2019 Aug 7): .

    Scientific reports
    Moon IS
    Scientific reports 9.1 (2019 Dec 12): 18919.

    WB References

    Neuromuscular disorders : NMD
    Vissing J
    Neuromuscular disorders : NMD 23.12 (2013 Dec): 981-5.

    Harefuah
    David M
    Harefuah 117.9 (1989 Nov 1): 273-4.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Goldberg AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.31 (2011 Aug 3): 11231-43.

    Scientific reports
    Noree C
    Scientific reports 8.1 (2018 Apr 18): 6186.

    PloS one
    Fanen P
    PloS one 6.3 (2011 Mar 31): e18334.

    Biology open
    Nose A
    Biology open 1.4 (2012 Apr 15): 362-75.

    Aging cell
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    The Journal of biological chemistry
    Pradet-Balade B
    The Journal of biological chemistry 289.9 (2014 Feb 28): 6236-47.

    Cell
    Janke C
    Cell 137.6 (2009 Jun 12): 1076-87.

    Alcohol (Fayetteville, N.Y.)
    McCaskill M
    Alcohol (Fayetteville, N.Y.) 80. (2019 Nov): 99-108.

    Nature communications
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Traffic (Copenhagen, Denmark)
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    The Journal of infectious diseases
    Guttman JA
    The Journal of infectious diseases 219.1 (2019 Jan 1): 145-153.

    IHC References

    Current biology : CB
    Gaertig J
    Current biology : CB 20.5 (2010 Mar 9): 435-40.

    Eukaryotic cell
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    Parasitology
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    In vitro cellular & developmental biology. Animal
    Moon IS
    In vitro cellular & developmental biology. Animal 48.8 (2012 Sep): 535-44.

    Cell
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    FFPE References

    The Journal of comparative neurology
    Wolff G
    The Journal of comparative neurology 520.13 (2012 Sep 1): 2847-63.

    Journal of anatomy
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Aging cell
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    All References

    Current biology : CB
    Gaertig J
    Current biology : CB 20.5 (2010 Mar 9): 435-40.

    Eukaryotic cell
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    Parasitology
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    In vitro cellular & developmental biology. Animal
    Moon IS
    In vitro cellular & developmental biology. Animal 48.8 (2012 Sep): 535-44.

    Cell
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna
    Manolescu N
    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna 39.6 (1987 Nov-Dec): 513-20.

    Developmental biology
    Piano F
    Developmental biology 335.1 (2009 Nov 1): 253-62.

    Developmental cell
    Weis WI
    Developmental cell 23.3 (2012 Sep 11): 533-46.

    Journal of morphology
    Page LR
    Journal of morphology 278.1 (2017 Jan): 29-49.

    The Journal of comparative neurology
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    The American journal of Chinese medicine
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Journal of cell science
    Pearson CG
    Journal of cell science 132.15 (2019 Aug 7): .

    Scientific reports
    Moon IS
    Scientific reports 9.1 (2019 Dec 12): 18919.

    Neuromuscular disorders : NMD
    Vissing J
    Neuromuscular disorders : NMD 23.12 (2013 Dec): 981-5.

    Harefuah
    David M
    Harefuah 117.9 (1989 Nov 1): 273-4.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Goldberg AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.31 (2011 Aug 3): 11231-43.

    Scientific reports
    Noree C
    Scientific reports 8.1 (2018 Apr 18): 6186.

    PloS one
    Fanen P
    PloS one 6.3 (2011 Mar 31): e18334.

    Biology open
    Nose A
    Biology open 1.4 (2012 Apr 15): 362-75.

    Aging cell
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    The Journal of biological chemistry
    Pradet-Balade B
    The Journal of biological chemistry 289.9 (2014 Feb 28): 6236-47.

    Cell
    Janke C
    Cell 137.6 (2009 Jun 12): 1076-87.

    Alcohol (Fayetteville, N.Y.)
    McCaskill M
    Alcohol (Fayetteville, N.Y.) 80. (2019 Nov): 99-108.

    Nature communications
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Traffic (Copenhagen, Denmark)
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    The Journal of infectious diseases
    Guttman JA
    The Journal of infectious diseases 219.1 (2019 Jan 1): 145-153.

    The Journal of comparative neurology
    Wolff G
    The Journal of comparative neurology 520.13 (2012 Sep 1): 2847-63.

    Journal of anatomy
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Nature cell biology
    Gaertig J
    Nature cell biology 4.3 (2002 Mar): 256-9.

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