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12G10 anti-alpha-tubulin

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$40.00
SKU: 12G10 anti-alpha-tubulin
View product citations for antibody 12G10 anti-alpha-tubulin on CiteAb

In Stock

Available: 237

DSHB Data Sheet

Catalog Fields

Antigen: tubulin (alpha-)
Hybridoma Cells Available: Yes
Antigen Species: Tetrahymena
Depositor: Frankel, J. / Nelsen, E.M.
Isotype: MIgG1
Antigen Sequence: 3139885 8020112
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Algae, C. elegans, Cephalopod, Dictyostelium, Drosophila, Fish, Hermit Crab, Human, Medusa, Mouse, Protozoa, Saccharomyces, Tetrahymena, Xenopus
Depositors Notes:
Antigen Molecular Weight: Predicted: 49.59 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ATU1
Immunogen: alpha tubulin, Tetrahymena thermophila and Tetrahymena pyriformis (mixture)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P41351 
Immunogen Sequence: alpha tubulin
Entrez Gene ID: 7838142 
Additional Characterization:
Antibody Registry ID: AB_1157911 
Additional Information:
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12G10 anti-alpha-tubulin was deposited to the DSHB by Frankel, J. / Nelsen, E.M. (DSHB Hybridoma Product 12G10 anti-alpha-tubulin)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

28 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Hyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell.
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    [Digestive tract involvement in collagenoses].
    Manolescu N
    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna 39.6 (1987 Nov-Dec): 513-20.

    Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro.
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    A family of protein-deglutamylating enzymes associated with neurodegeneration.
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    Evolution of early embryogenesis in rhabditid nematodes.
    Piano F
    Developmental biology 335.1 (2009 Nov 1): 253-62.

    α-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium.
    Weis WI
    Developmental cell 23.3 (2012 Sep 11): 533-46.

    Muscle and nerve net organization in stalked jellyfish (Medusozoa: Staurozoa).
    Page LR
    Journal of morphology 278.1 (2017 Jan): 29-49.

    Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods.
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods.
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons.
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Microtubule glycylation promotes attachment of basal bodies to the cell cortex.
    Pearson CG
    Journal of cell science 132.15 (2019 Aug 7): .

    WB References

    Muscle biopsies off-set normal cellular signaling in surrounding musculature.
    Vissing J
    Neuromuscular disorders : NMD 23.12 (2013 Dec): 981-5.

    [Darker side of tanning from artificial radiation source].
    David M
    Harefuah 117.9 (1989 Nov 1): 273-4.

    In situ visualization of protein interactions in sensory neurons: glutamic acid-rich proteins (GARPs) play differential roles for photoreceptor outer segment scaffolding.
    Goldberg AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.31 (2011 Aug 3): 11231-43.

    Extramitochondrial Assembly of Mitochondrial Targeting Signal Disrupted Mitochondrial Enzyme Aldehyde Dehydrogenase.
    Noree C
    Scientific reports 8.1 (2018 Apr 18): 6186.

    COMMD1-mediated ubiquitination regulates CFTR trafficking.
    Fanen P
    PloS one 6.3 (2011 Mar 31): e18334.

    Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction.
    Nose A
    Biology open 1.4 (2012 Apr 15): 362-75.

    SIRT6 protects against pathological damage caused by diet-induced obesity.
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
    Pradet-Balade B
    The Journal of biological chemistry 289.9 (2014 Feb 28): 6236-47.

    Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation.
    Janke C
    Cell 137.6 (2009 Jun 12): 1076-87.

    Diallyl disulfide inhibits ethanol-induced pulmonary cell vitamin D and antimicrobial peptide cathelicidin depletion.
    McCaskill M
    Alcohol (Fayetteville, N.Y.) 80. (2019 Nov): 99-108.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    Listeria Membrane Protrusion Collapse: Requirement of Cyclophilin A for Listeria Cell-to-Cell Spreading.
    Guttman JA
    The Journal of infectious diseases 219.1 (2019 Jan 1): 145-153.

    IHC References
    FFPE References
    All References

    Tubulin glutamylation regulates ciliary motility by altering inner dynein arm activity.
    Gaertig J
    Current biology : CB 20.5 (2010 Mar 9): 435-40.

    Hyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell.
    Gaertig J
    Eukaryotic cell 9.1 (2010 Jan): 184-93.

    Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro.
    Guionaud C
    Parasitology 140.8 (2013 Jul): 1033-50.

    A brown alga Sargassum fulvellum facilitates neuronal maturation and synaptogenesis.
    Moon IS
    In vitro cellular and developmental biology. Animal 48.8 (2012 Sep): 535-44.

    A family of protein-deglutamylating enzymes associated with neurodegeneration.
    Janke C
    Cell 143.4 (2010 Nov 12): 564-78.

    [Digestive tract involvement in collagenoses].
    Manolescu N
    Revista de medicina interna, neurologe, psihiatrie, neurochirurgie, dermato-venerologie. Medicina interna 39.6 (1987 Nov-Dec): 513-20.

    Evolution of early embryogenesis in rhabditid nematodes.
    Piano F
    Developmental biology 335.1 (2009 Nov 1): 253-62.

    α-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium.
    Weis WI
    Developmental cell 23.3 (2012 Sep 11): 533-46.

    Muscle and nerve net organization in stalked jellyfish (Medusozoa: Staurozoa).
    Page LR
    Journal of morphology 278.1 (2017 Jan): 29-49.

    Three-dimensional brain atlas of pygmy squid, Idiosepius paradoxus, revealing the largest relative vertical lobe system volume among the cephalopods.
    Tanaka NK
    The Journal of comparative neurology 524.10 (2016 Jul 1): 2142-57.

    Calotropis gigantea Promotes Neuritogenesis and Synaptogenesis through Activation of NGF-TrkA-Erk1/2 Signaling in Rat Hippocampal Neurons.
    Moon IS
    The American journal of Chinese medicine 46.8 (2018): 1861-1877.

    Microtubule glycylation promotes attachment of basal bodies to the cell cortex.
    Pearson CG
    Journal of cell science 132.15 (2019 Aug 7): .

    Muscle biopsies off-set normal cellular signaling in surrounding musculature.
    Vissing J
    Neuromuscular disorders : NMD 23.12 (2013 Dec): 981-5.

    [Darker side of tanning from artificial radiation source].
    David M
    Harefuah 117.9 (1989 Nov 1): 273-4.

    In situ visualization of protein interactions in sensory neurons: glutamic acid-rich proteins (GARPs) play differential roles for photoreceptor outer segment scaffolding.
    Goldberg AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.31 (2011 Aug 3): 11231-43.

    Extramitochondrial Assembly of Mitochondrial Targeting Signal Disrupted Mitochondrial Enzyme Aldehyde Dehydrogenase.
    Noree C
    Scientific reports 8.1 (2018 Apr 18): 6186.

    COMMD1-mediated ubiquitination regulates CFTR trafficking.
    Fanen P
    PloS one 6.3 (2011 Mar 31): e18334.

    Lola regulates glutamate receptor expression at the Drosophila neuromuscular junction.
    Nose A
    Biology open 1.4 (2012 Apr 15): 362-75.

    SIRT6 protects against pathological damage caused by diet-induced obesity.
    Cohen HY
    Aging cell 9.2 (2010 Apr): 162-73.

    Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
    Pradet-Balade B
    The Journal of biological chemistry 289.9 (2014 Feb 28): 6236-47.

    Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation.
    Janke C
    Cell 137.6 (2009 Jun 12): 1076-87.

    Diallyl disulfide inhibits ethanol-induced pulmonary cell vitamin D and antimicrobial peptide cathelicidin depletion.
    McCaskill M
    Alcohol (Fayetteville, N.Y.) 80. (2019 Nov): 99-108.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    Listeria Membrane Protrusion Collapse: Requirement of Cyclophilin A for Listeria Cell-to-Cell Spreading.
    Guttman JA
    The Journal of infectious diseases 219.1 (2019 Jan 1): 145-153.

    Fine structural organization of the hemiellipsoid body of the land hermit crab, Coenobita clypeatus.
    Wolff G
    The Journal of comparative neurology 520.13 (2012 Sep 1): 2847-63.

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Polyglycylation domain of beta-tubulin maintains axonemal architecture and affects cytokinesis in Tetrahymena.
    Gaertig J
    Nature cell biology 4.3 (2002 Mar): 256-9.

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