5A6

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$40.00
SKU: 5A6
View product citations for antibody 5A6 on CiteAb

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Available: 85

DSHB Data Sheet

Catalog Fields

Antigen: Tau
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Johnson, G.V.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Rochester Medical Center
Positive Tested Species Reactivity: Drosophila, Human
Depositors Notes:
Antigen Molecular Weight: 45-58 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MAPT
Immunogen: human recombinant/longest brain isoform 441aa
Alternate Gene Names: MAPTL, MTBT1, TAU
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence: 19-46
Uniprot ID: P10636 
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528487 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5A6 was deposited to the DSHB by Johnson, G.V.W. (DSHB Hybridoma Product 5A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Serotonergic dystrophy induced by excess serotonin.
    Condron BG
    Molecular and cellular neurosciences 44.3 (2010 Jul): 297-306.

    Lrrk promotes tau neurotoxicity through dysregulation of actin and mitochondrial dynamics.
    Feany MB
    PLoS biology 16.12 (2018 Dec): e2006265.

    CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus.
    Czeisler CM
    The American journal of pathology 189.2 (2019 Feb): 426-439.

    WB References

    Embryonic stem cell-based modeling of tau pathology in human neurons.
    Koch P
    The American journal of pathology 182.5 (2013 May): 1769-79.

    The tau protein in human cerebrospinal fluid in Alzheimer's disease consists of proteolytically derived fragments.
    Galasko D
    Journal of neurochemistry 68.1 (1997 Jan): 430-3.

    Insulin transiently increases tau phosphorylation: involvement of glycogen synthase kinase-3beta and Fyn tyrosine kinase.
    Johnson GV
    Journal of neurochemistry 72.2 (1999 Feb): 576-84.

    Cytoskeleton proteins are modulators of mutant tau-induced neurodegeneration in Drosophila.
    Lecourtois M
    Human molecular genetics 16.5 (2007 Mar 1): 555-66.

    Multiple-motor based transport and its regulation by Tau.
    Gross SP
    Proceedings of the National Academy of Sciences of the United States of America 104.1 (2007 Jan 2): 87-92.

    IP References

    The tau protein in human cerebrospinal fluid in Alzheimer's disease consists of proteolytically derived fragments.
    Galasko D
    Journal of neurochemistry 68.1 (1997 Jan): 430-3.

    Aminopeptidases do not directly degrade tau protein.
    Hersh LB
    Molecular neurodegeneration 5. (2010 Nov 5): 48.

    Epitope Map References

    Early N-terminal changes and caspase-6 cleavage of tau in Alzheimer's disease.
    Binder LI
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.36 (2004 Sep 8): 7895-902.

    All References

    Serotonergic dystrophy induced by excess serotonin.
    Condron BG
    Molecular and cellular neurosciences 44.3 (2010 Jul): 297-306.

    Lrrk promotes tau neurotoxicity through dysregulation of actin and mitochondrial dynamics.
    Feany MB
    PLoS biology 16.12 (2018 Dec): e2006265.

    CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus.
    Czeisler CM
    The American journal of pathology 189.2 (2019 Feb): 426-439.

    Embryonic stem cell-based modeling of tau pathology in human neurons.
    Koch P
    The American journal of pathology 182.5 (2013 May): 1769-79.

    The tau protein in human cerebrospinal fluid in Alzheimer's disease consists of proteolytically derived fragments.
    Galasko D
    Journal of neurochemistry 68.1 (1997 Jan): 430-3.

    Insulin transiently increases tau phosphorylation: involvement of glycogen synthase kinase-3beta and Fyn tyrosine kinase.
    Johnson GV
    Journal of neurochemistry 72.2 (1999 Feb): 576-84.

    Cytoskeleton proteins are modulators of mutant tau-induced neurodegeneration in Drosophila.
    Lecourtois M
    Human molecular genetics 16.5 (2007 Mar 1): 555-66.

    Multiple-motor based transport and its regulation by Tau.
    Gross SP
    Proceedings of the National Academy of Sciences of the United States of America 104.1 (2007 Jan 2): 87-92.

    Aminopeptidases do not directly degrade tau protein.
    Hersh LB
    Molecular neurodegeneration 5. (2010 Nov 5): 48.

    Early N-terminal changes and caspase-6 cleavage of tau in Alzheimer's disease.
    Binder LI
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.36 (2004 Sep 8): 7895-902.

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