JLT12

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$40.00
SKU: JLT12
View product citations for antibody JLT12 on CiteAb

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Available: 153

DSHB Data Sheet

Catalog Fields

Antigen: troponin T, fast skeletal muscle specific
Hybridoma Cells Available: Yes
Antigen Species: Rabbit
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Axolotl, Bovine, Broad species, Chicken, Human, Rabbit, Rat
Depositors Notes: Paraformaldehyde fixation for immunostaining is recommended
Antigen Molecular Weight: Multiple isoforms; Predicted: 29 to 33 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TNNT3
Immunogen: Skeletal muscle troponin T (TnT)
Alternate Gene Names: TnTf, fTnT
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 71-151 of TnT2f
Uniprot ID: P02641 
Immunogen Sequence: Full length protein
Entrez Gene ID: 100009527 
Additional Characterization:  
Antibody Registry ID: AB_2618103 
Additional Information: JLT12 recognizes all Troponin T, fast skeletal muscle isoforms and doesn't recognize slow isoforms. This antibody was initially characterized in Lin et al. [Lin, J.J.-C., Feramisco, J.R., Blose, S.H., and Matsumura, F. (1984). Monoclonal antibodies to cytoskeletal proteins. In Monoclonal Antibodies and Hybridomas: Progress and Applications. (eds. R.H. Kennett, T.J. McKearn, and K.B. Bechtol) pp. 119-151, Plenum Press, New York].
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLT12 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLT12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T.
    Schachat FH
    Journal of muscle research and cell motility 8.1 (1987 Feb): 1-12.

    IF References

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    WB References

    The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T.
    Schachat FH
    Journal of muscle research and cell motility 8.1 (1987 Feb): 1-12.

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    Expression of a novel combination of fast and slow troponin T isoforms in rabbit extraocular muscles.
    Schachat FH
    Journal of muscle research and cell motility 9.3 (1988 Jun): 241-7.

    Origin of fetal troponin T: developmentally regulated splicing of a new exon in the fast troponin T gene.
    Schachat F
    Developmental biology 158.2 (1993 Aug): 503-9.

    Transitions from fetal to fast troponin T isoforms are coordinated with changes in tropomyosin and alpha-actinin isoforms in developing rabbit skeletal muscle.
    Schachat F
    Developmental biology 140.2 (1990 Aug): 253-60.

    Patterns of troponin T expression in mammalian fast, slow and promiscuous muscle fibres.
    Schachat FH
    Journal of muscle research and cell motility 8.1 (1987 Feb): 13-22.

    A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression.
    Atkins DL
    Cardiovascular research 41.1 (1999 Jan): 200-11.

    Molecular and immunohistochemical analyses of cardiac troponin T during cardiac development in the Mexican axolotl, Ambystoma mexicanum.
    Lemanski LF
    Journal of cellular biochemistry 100.1 (2007 Jan 1): 1-15.

    Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function.
    Lang CH
    Alcoholism, clinical and experimental research 43.10 (2019 Oct): 2090-2099.

    Epitope Map References

    The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T.
    Schachat FH
    Journal of muscle research and cell motility 8.1 (1987 Feb): 1-12.

    All References

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T.
    Schachat FH
    Journal of muscle research and cell motility 8.1 (1987 Feb): 1-12.

    Expression of a novel combination of fast and slow troponin T isoforms in rabbit extraocular muscles.
    Schachat FH
    Journal of muscle research and cell motility 9.3 (1988 Jun): 241-7.

    Origin of fetal troponin T: developmentally regulated splicing of a new exon in the fast troponin T gene.
    Schachat F
    Developmental biology 158.2 (1993 Aug): 503-9.

    Transitions from fetal to fast troponin T isoforms are coordinated with changes in tropomyosin and alpha-actinin isoforms in developing rabbit skeletal muscle.
    Schachat F
    Developmental biology 140.2 (1990 Aug): 253-60.

    Patterns of troponin T expression in mammalian fast, slow and promiscuous muscle fibres.
    Schachat FH
    Journal of muscle research and cell motility 8.1 (1987 Feb): 13-22.

    A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression.
    Atkins DL
    Cardiovascular research 41.1 (1999 Jan): 200-11.

    Molecular and immunohistochemical analyses of cardiac troponin T during cardiac development in the Mexican axolotl, Ambystoma mexicanum.
    Lemanski LF
    Journal of cellular biochemistry 100.1 (2007 Jan 1): 1-15.

    Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function.
    Lang CH
    Alcoholism, clinical and experimental research 43.10 (2019 Oct): 2090-2099.

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