JLT12
Catalog Fields
Antigen: troponin T, fast skeletal muscle specific
Hybridoma Cells Available: Yes
Antigen Species: Rabbit
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Axolotl, Bovine, Broad species, Chicken, Human, Rabbit, Rat
Depositors Notes: Paraformaldehyde fixation for immunostaining is recommended
Predicted Species Reactivity:
Gene: TNNT3
Immunogen: Skeletal muscle troponin T (TnT)
Alternate Gene Names: TnTf, fTnT
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 71-151 of TnT2f
Uniprot ID: P02641
Immunogen Sequence: Full length protein
Entrez Gene ID: 100009527
Antibody Registry ID: AB_2618103
Additional Information: JLT12 recognizes all Troponin T, fast skeletal muscle isoforms and doesn't recognize slow isoforms. This antibody was initially characterized in Lin et al. [Lin, J.J.-C., Feramisco, J.R., Blose, S.H., and Matsumura, F. (1984). Monoclonal antibodies to cytoskeletal proteins. In Monoclonal Antibodies and Hybridomas: Progress and Applications. (eds. R.H. Kennett, T.J. McKearn, and K.B. Bechtol) pp. 119-151, Plenum Press, New York].
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLT12 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLT12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T.
Schachat FH
Journal of muscle research and cell motility 8.1 (1987 Feb): 1-12.