JLT12
Catalog Fields
Antigen: troponin T, fast skeletal muscle specific
Hybridoma Cells Available: Yes
Antigen Species: Rabbit
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: Paraformaldehyde fixation for immunostaining is recommended
Positive Tested Species Reactivity: Axolotl, Bovine, Broad species, Chicken, Human, Rabbit, Rat
Gene: TNNT3
Antigen Molecular Weight: Multiple isoforms; Predicted: 29 to 33 kDa
Alternate Gene Names: TnTf, fTnT
Immunogen: Skeletal muscle troponin T (TnT)
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: P02641
Epitope Location or Sequence: aa 71-151 of TnT2f
Entrez Gene ID: 100009527
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2618103
Additional Information: JLT12 recognizes all Troponin T, fast skeletal muscle isoforms and doesn't recognize slow isoforms. This antibody was initially characterized in Lin et al. [Lin, J.J.-C., Feramisco, J.R., Blose, S.H., and Matsumura, F. (1984). Monoclonal antibodies to cytoskeletal proteins. In Monoclonal Antibodies and Hybridomas: Progress and Applications. (eds. R.H. Kennett, T.J. McKearn, and K.B. Bechtol) pp. 119-151, Plenum Press, New York].
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JLT12 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product JLT12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
9 References
Initial Publication
The extent of amino-terminal heterogeneity in rabbit fast skeletal muscle troponin T.
Schachat FH
Journal of muscle research and cell motility 8.1 (1987 Feb): 1-12.