F5D

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SKU: F5D

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DSHB Data Sheet

Catalog Fields

Product Name/ID: F5D
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Myog
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2146602 
Uniprot ID: P20428 
RRID:  
Entrez Gene ID: 29148 
Clonality: Monoclonal
Immunogen: GST-fusion protein
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Myogenin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/10/1996
Antigen Molecular Weight: Predicted: 24kDa; apparent: 34 kDa
Depositor: Wright, W.E.
Antigen Sequence:
Depositor Institution: University of Texas Southwestern Medical Center
Antigen Species: Rat
Depositor Notes: This antibody immunoprecipitates deletion mutants containing a.a. 138-158, region immediately carboxy-terminal to the bHLH domain.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Feline, Human, Mammal, Mouse, Porcine, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F5D was deposited to the DSHB by Wright, W.E. (DSHB Hybridoma Product F5D)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

35 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    Efficient myogenic reprogramming of adult white fat stem cells and bone marrow stem cells by freshly isolated skeletal muscle fibers.
    Patel K
    Translational research : the journal of laboratory and clinical medicine 158.6 (2011 Dec): 334-43.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Ck2-Dependent Phosphorylation Is Required to Maintain Pax7 Protein Levels in Proliferating Muscle Progenitors.
    Olguín HC
    PloS one 11.5 (2016): e0154919.

    Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal.
    Borycki AG
    Stem cell reports 6.6 (2016 Jun 14): 798-805.

    Satellite cells fail to contribute to muscle repair but are functional in Pompe disease (glycogenosis type II).
    Colle MA
    Acta neuropathologica communications 6.1 (2018 Oct 31): 116.

    Orai1 inhibitor STIM2β regulates myogenesis by controlling SOCE dependent transcriptional factors.
    Park CY
    Scientific reports 9.1 (2019 Jul 25): 10794.

    Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration.
    Hasson P
    Developmental cell 53.4 (2020 May 18): 406-417.e5.

    Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling.
    Albini S
    Skeletal muscle 10.1 (2020 May 2): 13.

    Muscle-derived TRAIL negatively regulates myogenic differentiation.
    Chen J
    Experimental cell research 394.1 (2020 Sep 1): 112165.

    Avian Satellite Cell Plasticity.
    Kempisty B
    Animals : an open access journal from MDPI 10.8 (2020 Jul 31): .

    Phosphatase orphan 1 inhibits myoblast proliferation and promotes myogenic differentiation.
    Kuang S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.1 (2021 Jan): e21154.

    Myogenin is required for assembly of the transcription machinery on muscle genes during skeletal muscle differentiation.
    Davie J
    PloS one 16.1 (2021): e0245618.

    Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion.
    Rape M
    Developmental cell 56.5 (2021 Mar 8): 588-601.e9.



    . (): .

    Patient-specific iPSC-derived cellular models of LGMDR1.
    López de Munain A
    Stem cell research 53. (2021 May): 102333.

    Loss of p16/Ink4a drives high frequency of rhabdomyosarcoma in a rat model of Duchenne muscular dystrophy.
    Yamanouchi K
    The Journal of veterinary medical science 83.9 (2021 Sep 15): 1416-1424.

    WB References

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    Loss of MEF2D expression inhibits differentiation and contributes to oncogenesis in rhabdomyosarcoma cells.
    Davie J
    Molecular cancer 12.1 (2013 Nov 27): 150.

    Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development.
    Zhao QJ
    Scientific reports 6. (2016 Jul 25): 30281.

    A nitrate ester of sedative alkyl alcohol improves muscle function and structure in a murine model of Duchenne muscular dystrophy.
    Lu Q
    Molecular pharmaceutics 10.10 (2013 Oct 7): 3862-70.

    Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.
    Sabourin LA
    Skeletal muscle 3.1 (2013 Jul 1): 16.

    Hedgehog signaling regulates MyoD expression and activity.
    Skerjanc IS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4389-404.

    Interferon-γ resets muscle cell fate by stimulating the sequential recruitment of JARID2 and PRC2 to promoters to repress myogenesis.
    Davie JK
    Science signaling 6.305 (2013 Dec 10): ra107.

    Orai1 inhibitor STIM2β regulates myogenesis by controlling SOCE dependent transcriptional factors.
    Park CY
    Scientific reports 9.1 (2019 Jul 25): 10794.

    Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration.
    Hasson P
    Developmental cell 53.4 (2020 May 18): 406-417.e5.

    Phosphatase orphan 1 inhibits myoblast proliferation and promotes myogenic differentiation.
    Kuang S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.1 (2021 Jan): e21154.

    Magnesium supplementation enhances mTOR signalling to facilitate myogenic differentiation and improve aged muscle performance.
    Peng S
    Bone 146. (2021 May): 115886.

    The Bromodomains of the mammalian SWI/SNF (mSWI/SNF) ATPases Brahma (BRM) and Brahma Related Gene 1 (BRG1) promote chromatin interaction and are critical for skeletal muscle differentiation.
    Imbalzano AN
    Nucleic acids research 49.14 (2021 Aug 20): 8060-8077.

    IHC References
    IP References
    ChIP References
    ELISA References

    MyoD, myogenin independent differentiation of primordial myoblasts in mouse somites.
    Buckingham M
    The Journal of cell biology 116.5 (1992 Mar): 1243-55.

    FFPE References

    Histological and histochemical analysis of embryoid bodies.
    Mokrý J
    Acta histochemica 104.4 (2002): 361-5.

    All References

    c-MET regulates myoblast motility and myocyte fusion during adult skeletal muscle regeneration.
    Fan CM
    PloS one 8.11 (2013): e81757.

    Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.
    Sabourin LA
    Skeletal muscle 3.1 (2013 Jul 1): 16.

    A rat model for muscle regeneration in the soft palate.
    Wagener FA
    PloS one 8.3 (2013): e59193.

    Self-organized formation of developing appendages from murine pluripotent stem cells.
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    Efficient myogenic reprogramming of adult white fat stem cells and bone marrow stem cells by freshly isolated skeletal muscle fibers.
    Patel K
    Translational research : the journal of laboratory and clinical medicine 158.6 (2011 Dec): 334-43.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Ck2-Dependent Phosphorylation Is Required to Maintain Pax7 Protein Levels in Proliferating Muscle Progenitors.
    Olguín HC
    PloS one 11.5 (2016): e0154919.

    Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal.
    Borycki AG
    Stem cell reports 6.6 (2016 Jun 14): 798-805.

    Satellite cells fail to contribute to muscle repair but are functional in Pompe disease (glycogenosis type II).
    Colle MA
    Acta neuropathologica communications 6.1 (2018 Oct 31): 116.

    Orai1 inhibitor STIM2β regulates myogenesis by controlling SOCE dependent transcriptional factors.
    Park CY
    Scientific reports 9.1 (2019 Jul 25): 10794.

    Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration.
    Hasson P
    Developmental cell 53.4 (2020 May 18): 406-417.e5.

    Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling.
    Albini S
    Skeletal muscle 10.1 (2020 May 2): 13.

    Muscle-derived TRAIL negatively regulates myogenic differentiation.
    Chen J
    Experimental cell research 394.1 (2020 Sep 1): 112165.

    Avian Satellite Cell Plasticity.
    Kempisty B
    Animals : an open access journal from MDPI 10.8 (2020 Jul 31): .

    Phosphatase orphan 1 inhibits myoblast proliferation and promotes myogenic differentiation.
    Kuang S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35.1 (2021 Jan): e21154.

    Myogenin is required for assembly of the transcription machinery on muscle genes during skeletal muscle differentiation.
    Davie J
    PloS one 16.1 (2021): e0245618.

    Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion.
    Rape M
    Developmental cell 56.5 (2021 Mar 8): 588-601.e9.



    . (): .

    Patient-specific iPSC-derived cellular models of LGMDR1.
    López de Munain A
    Stem cell research 53. (2021 May): 102333.

    Loss of p16/Ink4a drives high frequency of rhabdomyosarcoma in a rat model of Duchenne muscular dystrophy.
    Yamanouchi K
    The Journal of veterinary medical science 83.9 (2021 Sep 15): 1416-1424.

    Loss of MEF2D expression inhibits differentiation and contributes to oncogenesis in rhabdomyosarcoma cells.
    Davie J
    Molecular cancer 12.1 (2013 Nov 27): 150.

    Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development.
    Zhao QJ
    Scientific reports 6. (2016 Jul 25): 30281.

    A nitrate ester of sedative alkyl alcohol improves muscle function and structure in a murine model of Duchenne muscular dystrophy.
    Lu Q
    Molecular pharmaceutics 10.10 (2013 Oct 7): 3862-70.

    Hedgehog signaling regulates MyoD expression and activity.
    Skerjanc IS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4389-404.

    Interferon-γ resets muscle cell fate by stimulating the sequential recruitment of JARID2 and PRC2 to promoters to repress myogenesis.
    Davie JK
    Science signaling 6.305 (2013 Dec 10): ra107.

    Magnesium supplementation enhances mTOR signalling to facilitate myogenic differentiation and improve aged muscle performance.
    Peng S
    Bone 146. (2021 May): 115886.

    The Bromodomains of the mammalian SWI/SNF (mSWI/SNF) ATPases Brahma (BRM) and Brahma Related Gene 1 (BRG1) promote chromatin interaction and are critical for skeletal muscle differentiation.
    Imbalzano AN
    Nucleic acids research 49.14 (2021 Aug 20): 8060-8077.

    Gamma interferon modulates myogenesis through the major histocompatibility complex class II transactivator, CIITA.
    Davie JK
    Molecular and cellular biology 31.14 (2011 Jul): 2854-66.

    Myogenin recruits the histone chaperone facilitates chromatin transcription (FACT) to promote nucleosome disassembly at muscle-specific genes.
    Davie JK
    The Journal of biological chemistry 288.11 (2013 Mar 15): 7676-7687.

    Sequential association of myogenic regulatory factors and E proteins at muscle-specific genes.
    Davie JK
    Skeletal muscle 1.1 (2011 Apr 4): 14.

    MyoD, myogenin independent differentiation of primordial myoblasts in mouse somites.
    Buckingham M
    The Journal of cell biology 116.5 (1992 Mar): 1243-55.

    Histological and histochemical analysis of embryoid bodies.
    Mokrý J
    Acta histochemica 104.4 (2002): 361-5.

    Cyclic amplification and selection of targets (CASTing) for the myogenin consensus binding site.
    Funk W
    Molecular and cellular biology 11.8 (1991 Aug): 4104-10.

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