F5D

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$40.00
SKU: F5D
View product citations for antibody F5D on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myogenin
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Wright, W.E.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Texas Southwestern Medical Center
Positive Tested Species Reactivity: Canine, Feline, Human, Mammal, Mouse, Porcine, Rat
Depositors Notes: This antibody immunoprecipitates deletion mutants containing a.a. 138-158, region immediately carboxy-terminal to the bHLH domain.
Antigen Molecular Weight: Predicted: 24kDa; apparent: 34 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Myog
Immunogen: GST-fusion protein
Alternate Gene Names: rCG_46454
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P20428 
Immunogen Sequence:
Entrez Gene ID: 29148 
Additional Characterization:
Antibody Registry ID: AB_2146602 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F5D was deposited to the DSHB by Wright, W.E. (DSHB Hybridoma Product F5D)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    ELISA References

    MyoD, myogenin independent differentiation of primordial myoblasts in mouse somites.
    Buckingham M
    The Journal of cell biology 116.5 (1992 Mar): 1243-55.

    FFPE References

    Histological and histochemical analysis of embryoid bodies.
    Mokrý J
    Acta histochemica 104.4 (2002): 361-5.

    All References

    c-MET regulates myoblast motility and myocyte fusion during adult skeletal muscle regeneration.
    Fan CM
    PloS one 8.11 (2013): e81757.

    Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.
    Sabourin LA
    Skeletal muscle 3.1 (2013 Jul 1): 16.

    A rat model for muscle regeneration in the soft palate.
    Wagener FA
    PloS one 8.3 (2013): e59193.

    Self-organized formation of developing appendages from murine pluripotent stem cells.
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    Murine muscle engineered from dermal precursors: an in vitro model for skeletal muscle generation, degeneration, and fatty infiltration.
    López de Munain A
    Tissue engineering. Part C, Methods 20.1 (2014 Jan): 28-41.

    Efficient myogenic reprogramming of adult white fat stem cells and bone marrow stem cells by freshly isolated skeletal muscle fibers.
    Patel K
    Translational research : the journal of laboratory and clinical medicine 158.6 (2011 Dec): 334-43.

    Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.
    Pourquié O
    Nature biotechnology 33.9 (2015 Sep): 962-9.

    Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Pourquié O
    Nature protocols 11.10 (2016 Oct): 1833-50.

    Ck2-Dependent Phosphorylation Is Required to Maintain Pax7 Protein Levels in Proliferating Muscle Progenitors.
    Olguín HC
    PloS one 11.5 (2016): e0154919.

    Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal.
    Borycki AG
    Stem cell reports 6.6 (2016 Jun 14): 798-805.

    Satellite cells fail to contribute to muscle repair but are functional in Pompe disease (glycogenosis type II).
    Colle MA
    Acta neuropathologica communications 6.1 (2018 Oct 31): 116.

    Orai1 inhibitor STIM2β regulates myogenesis by controlling SOCE dependent transcriptional factors.
    Park CY
    Scientific reports 9.1 (2019 Jul 25): 10794.

    Loss of MEF2D expression inhibits differentiation and contributes to oncogenesis in rhabdomyosarcoma cells.
    Davie J
    Molecular cancer 12.1 (2013 Nov 27): 150.

    Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development.
    Zhao QJ
    Scientific reports 6. (2016 Jul 25): 30281.

    A nitrate ester of sedative alkyl alcohol improves muscle function and structure in a murine model of Duchenne muscular dystrophy.
    Lu Q
    Molecular pharmaceutics 10.10 (2013 Oct 7): 3862-70.

    Hedgehog signaling regulates MyoD expression and activity.
    Skerjanc IS
    The Journal of biological chemistry 288.6 (2013 Feb 8): 4389-404.

    Interferon-γ resets muscle cell fate by stimulating the sequential recruitment of JARID2 and PRC2 to promoters to repress myogenesis.
    Davie JK
    Science signaling 6.305 (2013 Dec 10): ra107.

    Gamma interferon modulates myogenesis through the major histocompatibility complex class II transactivator, CIITA.
    Davie JK
    Molecular and cellular biology 31.14 (2011 Jul): 2854-66.

    Myogenin recruits the histone chaperone facilitates chromatin transcription (FACT) to promote nucleosome disassembly at muscle-specific genes.
    Davie JK
    The Journal of biological chemistry 288.11 (2013 Mar 15): 7676-87.

    MyoD, myogenin independent differentiation of primordial myoblasts in mouse somites.
    Buckingham M
    The Journal of cell biology 116.5 (1992 Mar): 1243-55.

    Histological and histochemical analysis of embryoid bodies.
    Mokrý J
    Acta histochemica 104.4 (2002): 361-5.

    Cyclic amplification and selection of targets (CASTing) for the myogenin consensus binding site.
    Funk W
    Molecular and cellular biology 11.8 (1991 Aug): 4104-10.

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