Antigen: troponin T (cardiac/slow)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Lin, J.J.-C.
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Broad species, Chicken, Fish, Human, Mouse, Rabbit, Rat, Xenopus
Depositors Notes: CT3 was initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990). Troponin T isoform switching during heart development. Ann. NY Acad. Sci. 588, 393-396.
Antigen Molecular Weight: Predicted (cTnT): 34 kDa; Apparent (cTnT): 43kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Purified cardiac troponin T
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 94-178
Uniprot ID: P13789
Immunogen Sequence: Full length protein
Entrez Gene ID: 286816
Antibody Registry ID: AB_528495
Additional Information: CT3 recognizes the embryonic and adult cardiac isforms [PMID: 2358124]. CT3 cross-react with slow skeletal muscle TnT but doesn't recognize fast skeletal muscle TnT [PMID: 12732643].
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CT3 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CT3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References