CT3

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$40.00
SKU: CT3
View product citations for antibody CT3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: troponin T (cardiac/slow)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Lin, J.J.-C.
Isotype: MIgG2a
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: CT3 was initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990). Troponin T isoform switching during heart development. Ann. NY Acad. Sci. 588, 393-396.
Positive Tested Species Reactivity: Broad species, Chicken, Fish, Human, Mouse, Rabbit, Rat, Xenopus
Gene: TNNT2
Antigen Molecular Weight: Predicted (cTnT): 34 kDa; Apparent (cTnT): 43kDa
Clonality: Monoclonal
Immunogen: Purified cardiac troponin T
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 94-178
Uniprot ID: P13789 
Immunogen Sequence: Full length protein
Entrez Gene ID: 286816 
Additional Information: CT3 recognizes the embryonic and adult cardiac isforms [PMID: 2358124]. CT3 cross-react with slow skeletal muscle TnT but doesn't recognize fast skeletal muscle TnT [PMID: 12732643].
Antibody Registry ID: AB_528495 
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CT3 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CT3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.
    Weinstein DC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.7 (2010 Jul): 1977-87.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    FACS References

    Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq.
    Kyba M
    Biochemical and biophysical research communications 474.3 (2016 Jun 3): 469-475.

    Epitope Map References

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    All References

    Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.
    Weinstein DC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.7 (2010 Jul): 1977-87.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo.
    Solursh M
    Developmental biology 140.1 (1990 Jul): 73-82.

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    Confocal imaging of early heart development in Xenopus laevis.
    Weeks DL
    Developmental biology 218.1 (2000 Feb 1): 64-73.

    Pitx2c attenuation results in cardiac defects and abnormalities of intestinal orientation in developing Xenopus laevis.
    Weeks DL
    Developmental biology 262.2 (2003 Oct 15): 268-81.

    Compensatory growth of healthy cardiac cells in the presence of diseased cells restores tissue homeostasis during heart development.
    Cox TC
    Developmental cell 15.4 (2008 Oct): 521-33.

    Requirement of a novel gene, Xin, in cardiac morphogenesis.
    Lin JJ
    Development (Cambridge, England) 126.6 (1999 Mar): 1281-94.

    Insulin-like growth factor (IGF) binding protein-5 blocks skeletal muscle differentiation by inhibiting IGF actions.
    Rotwein P
    Molecular endocrinology (Baltimore, Md.) 22.1 (2008 Jan): 206-15.

    Heart regeneration in adult <i>Xenopus tropicalis</i> after apical resection.
    Cai D
    Cell & bioscience 7. (2017): 70.

    Muscle specific fragile X related protein 1 isoforms are sequestered in the nucleus of undifferentiated myoblast.
    Khandjian EW
    BMC genetics 1. (2000): 4.

    Alternative RNA splicing-generated cardiac troponin T isoform switching: a non-heart-restricted genetic programming synchronized in developing cardiac and skeletal muscles.
    Jin JP
    Biochemical and biophysical research communications 225.3 (1996 Aug 23): 883-9.

    Conformational modulation of slow skeletal muscle troponin T by an NH(2)-terminal metal-binding extension.
    Huang QQ
    American journal of physiology. Cell physiology 279.4 (2000 Oct): C1067-77.

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq.
    Kyba M
    Biochemical and biophysical research communications 474.3 (2016 Jun 3): 469-475.

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