CT3
Catalog Fields
Clone ID/Product Name: CT3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TNNT2
Ab Isotype: MIgG2a
Gene Name:
Entrez Gene ID: 286816
Clonality: Monoclonal
Immunogen: Purified cardiac troponin T
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: troponin T (cardiac/slow)
Epitope Location or Sequence: aa 94-178
Alternate Antigen Name:
Deposit Date: 1/2/1996
Antigen Molecular Weight: Predicted (cTnT): 34 kDa; Apparent (cTnT): 43kDa
Depositor: Lin, J.J.-C.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Bovine
Depositor Notes: CT3 was initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990). Troponin T isoform switching during heart development. Ann. NY Acad. Sci. 588, 393-396.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Broad species, Chicken, Fish, Human, Mouse, Rabbit, Rat, Xenopus
Additional Information: CT3 recognizes the embryonic and adult cardiac isforms [PMID: 2358124]. CT3 cross-react with slow skeletal muscle TnT but doesn't recognize fast skeletal muscle TnT [PMID: 12732643].
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CT3 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CT3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo.
Solursh M
Developmental biology 140.1 (1990 Jul): 73-82.