MZ15

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$40.00
SKU: MZ15
View product citations for antibody MZ15 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: keratan sulfate
Hybridoma Cells Available: Yes
Antigen Species: Pig
Depositor: Watt, F.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cambridge Research Institute
Positive Tested Species Reactivity: Bovine, Chicken, Fish, Human, Mouse, Pig, Rabbit, Xenopus
Depositors Notes: Keratan sulphate proteoglycan competes with 125-I-proteoglycan monomer for MZ15 binding.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chondrocytes from larynx xartilage of 9 Month old pigs.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: The antigenic determinant recognized by MZ15 is part of the keratan sulfate chain that is sensitive to keratanase digestion and require the sulfate residues.
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_760352 
Additional Information: MZ15 recognizes both skeletal and corneal keratan sulfate.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MZ15 was deposited to the DSHB by Watt, F.M. (DSHB Hybridoma Product MZ15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus.
    Klymkowsky MW
    Scientific reports 5. (2015 May 27): 10283.

    The synovium-cartilage junction of the normal human knee. Implications for joint destruction and repair.
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    The fate of cells in the tailbud of Xenopus laevis.
    Kirschner MW
    Development (Cambridge, England) 127.2 (2000 Jan): 255-67.

    ELISA References
    FFPE References
    Epitope Map References
    All References

    Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus.
    Klymkowsky MW
    Scientific reports 5. (2015 May 27): 10283.

    The synovium-cartilage junction of the normal human knee. Implications for joint destruction and repair.
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    The fate of cells in the tailbud of Xenopus laevis.
    Kirschner MW
    Development (Cambridge, England) 127.2 (2000 Jan): 255-67.

    Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate.
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    Non-viral expression of mouse Oct4, Sox2, and Klf4 transcription factors efficiently reprograms tadpole muscle fibers in vivo.
    Coen L
    The Journal of biological chemistry 287.10 (2012 Mar 2): 7427-35.

    The spatial and temporal pattern of collagens I and II and keratan sulphate in the developing chick metatarsophalangeal joint.
    Archer CW
    Development (Cambridge, England) 99.3 (1987 Mar): 383-91.

    Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture.
    Watt FM
    Journal of cell science 89 ( Pt 3). (1988 Mar): 373-8.

    MZ15, a monoclonal antibody recognizing keratan sulphate, stains chick tendon.
    Archer CW
    The Histochemical journal 19.12 (1987 Dec): 651-7.

    Subpopulations of chondrocytes from different zones of pig articular cartilage. Isolation, growth and proteoglycan synthesis in culture.
    Watt FM
    Journal of cell science 97 ( Pt 2). (1990 Oct): 349-60.

    Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition.
    Davidson C
    The Journal of biological chemistry 274.22 (1999 May 28): 15892-900.

    The antigenic determinants recognized by three monoclonal antibodies to keratan sulphate involve sulphated hepta- or larger oligosaccharides of the poly(N-acetyllactosamine) series.
    Feizi T
    European journal of biochemistry 157.2 (1986 Jun 2): 385-91.

    Sulphate groups are involved in the antigenicity of keratan sulphate and mask i antigen expression on their poly-N-acetyllactosamine backbones. An immunochemical and chromatographic study of keratan sulphate oligosaccharides after desulphation or nitrosation.
    Feizi T
    European journal of biochemistry 160.3 (1986 Nov 3): 537-45.

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