MZ15

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SKU: MZ15

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: MZ15
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_760352 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Chondrocytes from larynx xartilage of 9 Month old pigs.
Clone:
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: keratan sulfate
Epitope Location or Sequence: The antigenic determinant recognized by MZ15 is part of the keratan sulfate chain that is sensitive to keratanase digestion and require the sulfate residues.
Alternate Antigen Name:
Deposit Date: 2/20/2007
Antigen Molecular Weight:
Depositor: Watt, F.M.
Antigen Sequence:
Depositor Institution: Cambridge Research Institute
Antigen Species: Pig
Depositor Notes: Keratan sulphate proteoglycan competes with 125-I-proteoglycan monomer for MZ15 binding.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Chicken, Fish, Human, Mouse, Pig, Rabbit, Rat, Xenopus
Additional Information: MZ15 recognizes both skeletal and corneal keratan sulfate.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MZ15 was deposited to the DSHB by Watt, F.M. (DSHB Hybridoma Product MZ15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus.
    Klymkowsky MW
    Scientific reports 5. (2015 May 27): 10283.

    The synovium-cartilage junction of the normal human knee. Implications for joint destruction and repair.
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    The fate of cells in the tailbud of Xenopus laevis.
    Kirschner MW
    Development (Cambridge, England) 127.2 (2000 Jan): 255-67.

    Imaging Keratan Sulfate in Ocular Tissue Sections by Immunofluorescence Microscopy and LA-ICP-MS.
    Tauber R
    ACS applied bio materials 5.2 (2022 Feb 21): 853-861.

    Posterior Embryotoxon Revisited: An Immunohistologic Study.
    Edward DP
    Ophthalmology. Glaucoma 5.4 (2022 Jul-Aug): 396-401.

    Bioinspired Injectable Hydrogels Dynamically Stiffen and Contract to Promote Mechanosensing-Mediated Chondrogenic Commitment of Stem Cells.
    Katti DS
    ACS applied materials & interfaces 14.6 (2022 Feb 16): 7531-7550.

    ELISA References
    FFPE References
    Epitope Map References
    All References

    Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus.
    Klymkowsky MW
    Scientific reports 5. (2015 May 27): 10283.

    The synovium-cartilage junction of the normal human knee. Implications for joint destruction and repair.
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    The fate of cells in the tailbud of Xenopus laevis.
    Kirschner MW
    Development (Cambridge, England) 127.2 (2000 Jan): 255-67.

    Imaging Keratan Sulfate in Ocular Tissue Sections by Immunofluorescence Microscopy and LA-ICP-MS.
    Tauber R
    ACS applied bio materials 5.2 (2022 Feb 21): 853-861.

    Posterior Embryotoxon Revisited: An Immunohistologic Study.
    Edward DP
    Ophthalmology. Glaucoma 5.4 (2022 Jul-Aug): 396-401.

    Bioinspired Injectable Hydrogels Dynamically Stiffen and Contract to Promote Mechanosensing-Mediated Chondrogenic Commitment of Stem Cells.
    Katti DS
    ACS applied materials & interfaces 14.6 (2022 Feb 16): 7531-7550.

    Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate.
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    Non-viral expression of mouse Oct4, Sox2, and Klf4 transcription factors efficiently reprograms tadpole muscle fibers in vivo.
    Coen L
    The Journal of biological chemistry 287.10 (2012 Mar 2): 7427-35.

    The spatial and temporal pattern of collagens I and II and keratan sulphate in the developing chick metatarsophalangeal joint.
    Archer CW
    Development (Cambridge, England) 99.3 (1987 Mar): 383-91.

    Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture.
    Watt FM
    Journal of cell science 89 ( Pt 3). (1988 Mar): 373-8.

    MZ15, a monoclonal antibody recognizing keratan sulphate, stains chick tendon.
    Archer CW
    The Histochemical journal 19.12 (1987 Dec): 651-7.

    Subpopulations of chondrocytes from different zones of pig articular cartilage. Isolation, growth and proteoglycan synthesis in culture.
    Watt FM
    Journal of cell science 97 ( Pt 2). (1990 Oct): 349-60.

    Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition.
    Davidson C
    The Journal of biological chemistry 274.22 (1999 May 28): 15892-900.

    The antigenic determinants recognized by three monoclonal antibodies to keratan sulphate involve sulphated hepta- or larger oligosaccharides of the poly(N-acetyllactosamine) series.
    Feizi T
    European journal of biochemistry 157.2 (1986 Jun 2): 385-91.

    Sulphate groups are involved in the antigenicity of keratan sulphate and mask i antigen expression on their poly-N-acetyllactosamine backbones. An immunochemical and chromatographic study of keratan sulphate oligosaccharides after desulphation or nitrosation.
    Feizi T
    European journal of biochemistry 160.3 (1986 Nov 3): 537-45.

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