MZ15
Catalog Fields
Product Name/ID: MZ15
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_760352
Clonality: Monoclonal
Immunogen: Chondrocytes from larynx xartilage of 9 Month old pigs.
Clone:
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: keratan sulfate
Epitope Location or Sequence: The antigenic determinant recognized by MZ15 is part of the keratan sulfate chain that is sensitive to keratanase digestion and require the sulfate residues.
Alternate Antigen Name:
Deposit Date: 2/20/2007
Antigen Molecular Weight:
Depositor: Watt, F.M.
Antigen Sequence:
Depositor Institution: Cambridge Research Institute
Antigen Species: Pig
Depositor Notes: Keratan sulphate proteoglycan competes with 125-I-proteoglycan monomer for MZ15 binding.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Chicken, Fish, Human, Mouse, Pig, Rabbit, Rat, Xenopus
Additional Information: MZ15 recognizes both skeletal and corneal keratan sulfate.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MZ15 was deposited to the DSHB by Watt, F.M. (DSHB Hybridoma Product MZ15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate.
Watt FM
The Journal of cell biology 101.1 (1985 Jul): 53-9.