MZ15

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$40.00
SKU: MZ15
View product citations for antibody MZ15 on CiteAb

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Available: 49

DSHB Data Sheet

Catalog Fields

Antigen: keratan sulfate
Hybridoma Cells Available: Yes
Antigen Species: Pig
Depositor: Watt, F.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cambridge Research Institute
Positive Tested Species Reactivity: Bovine, Chicken, Fish, Human, Mouse, Pig, Rabbit, Xenopus
Depositors Notes: Keratan sulphate proteoglycan competes with 125-I-proteoglycan monomer for MZ15 binding.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chondrocytes from larynx xartilage of 9 Month old pigs.
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: The antigenic determinant recognized by MZ15 is part of the keratan sulfate chain that is sensitive to keratanase digestion and require the sulfate residues.
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_760352 
Additional Information: MZ15 recognizes both skeletal and corneal keratan sulfate.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MZ15 was deposited to the DSHB by Watt, F.M. (DSHB Hybridoma Product MZ15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of cell biology
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    IF References

    The Journal of cell biology
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    The Journal of biological chemistry
    Coen L
    The Journal of biological chemistry 287.10 (2012 Mar 2): 7427-35.

    Development (Cambridge, England)
    Archer CW
    Development (Cambridge, England) 99.3 (1987 Mar): 383-91.

    Journal of cell science
    Watt FM
    Journal of cell science 89 ( Pt 3). (1988 Mar): 373-8.

    The Histochemical journal
    Archer CW
    The Histochemical journal 19.12 (1987 Dec): 651-7.

    Journal of cell science
    Watt FM
    Journal of cell science 97 ( Pt 2). (1990 Oct): 349-60.

    WB References

    The Journal of biological chemistry
    Davidson C
    The Journal of biological chemistry 274.22 (1999 May 28): 15892-900.

    Journal of cell science
    Watt FM
    Journal of cell science 97 ( Pt 2). (1990 Oct): 349-60.

    IHC References

    Scientific reports
    Klymkowsky MW
    Scientific reports 5. (2015 May 27): 10283.

    Arthritis and rheumatism
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    Development (Cambridge, England)
    Kirschner MW
    Development (Cambridge, England) 127.2 (2000 Jan): 255-67.

    ELISA References

    The Journal of cell biology
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    FFPE References

    Arthritis and rheumatism
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    Epitope Map References

    The Journal of cell biology
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    European journal of biochemistry
    Feizi T
    European journal of biochemistry 157.2 (1986 Jun 2): 385-91.

    European journal of biochemistry
    Feizi T
    European journal of biochemistry 160.3 (1986 Nov 3): 537-45.

    All References

    Scientific reports
    Klymkowsky MW
    Scientific reports 5. (2015 May 27): 10283.

    Arthritis and rheumatism
    Maini RN
    Arthritis and rheumatism 33.8 (1990 Aug): 1170-9.

    Development (Cambridge, England)
    Kirschner MW
    Development (Cambridge, England) 127.2 (2000 Jan): 255-67.

    The Journal of cell biology
    Watt FM
    The Journal of cell biology 101.1 (1985 Jul): 53-9.

    The Journal of biological chemistry
    Coen L
    The Journal of biological chemistry 287.10 (2012 Mar 2): 7427-35.

    Development (Cambridge, England)
    Archer CW
    Development (Cambridge, England) 99.3 (1987 Mar): 383-91.

    Journal of cell science
    Watt FM
    Journal of cell science 89 ( Pt 3). (1988 Mar): 373-8.

    The Histochemical journal
    Archer CW
    The Histochemical journal 19.12 (1987 Dec): 651-7.

    Journal of cell science
    Watt FM
    Journal of cell science 97 ( Pt 2). (1990 Oct): 349-60.

    The Journal of biological chemistry
    Davidson C
    The Journal of biological chemistry 274.22 (1999 May 28): 15892-900.

    European journal of biochemistry
    Feizi T
    European journal of biochemistry 157.2 (1986 Jun 2): 385-91.

    European journal of biochemistry
    Feizi T
    European journal of biochemistry 160.3 (1986 Nov 3): 537-45.

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