12/101

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$40.00
SKU: 12/101
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DSHB Data Sheet

Catalog Fields

Antigen: skeletal muscle marker, 102 kDa
Hybridoma Cells Available: Yes
Antigen Species: Newt
Depositor: Brockes, J.P.
Isotype: MIgG1
Antigen Sequence: membrane protein of unknown identity of the sarcoplasmic reticulum
Host Species: mouse
Depositors Institution: University College London
Positive Tested Species Reactivity: Adenovirus, Chicken, Fish, Mouse, Newt, Rabbit, Rat, Xenopus, Zebrafish
Depositors Notes:
Antigen Molecular Weight: Apparent: 102kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: regenerating skeletal muscle homogenate from newt limb
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1/SP2
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531892 
Additional Information: differentiated skeletal muscle marker (fast & intermediate fibers, somite-specific in embryos)
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12/101 was deposited to the DSHB by Brockes, J.P. (DSHB Hybridoma Product 12/101)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

162 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References

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    Use of fluorescent dextran conjugates as a long-term marker of osteogenic neural crest in frogs.
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    Developmental dynamics : an official publication of the American Association of Anatomists 230.1 (2004 May): 100-6.

    Patterns of spatial and temporal visceral arch muscle development in the Mexican axolotl (Ambystoma mexicanum).
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    Journal of morphology 261.2 (2004 Aug): 131-40.

    Heart myofibrillogenesis occurs in isolated chick posterior blastoderm: a culture model.
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    Biphasic myopathic phenotype of mouse DUX, an ORF within conserved FSHD-related repeats.
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    Kidins220/ARMS is dynamically expressed during Xenopus laevis development.
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    Skeletal muscle differentiation and fusion are regulated by the BAR-containing Rho-GTPase-activating protein (Rho-GAP), GRAF1.
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    Visualization of retinoic acid signaling in transgenic axolotls during limb development and regeneration.
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    Paraxial protocadherin coordinates cell polarity during convergent extension via Rho A and JNK.
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    Comparative pelvic development of the axolotl (Ambystoma mexicanum) and the Australian lungfish (Neoceratodus forsteri): conservation and innovation across the fish-tetrapod transition.
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    Asymmetric localization of numb in the chick somite and the influence of myogenic signals.
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    The Xenopus LIM-homeodomain protein Xlim5 regulates the differential adhesion properties of early ectoderm cells.
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    BMP-4 and Noggin signaling modulate dorsal fin and somite development in the axolotl trunk.
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    Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo.
    Dorey K
    Development (Cambridge, England) 137.23 (2010 Dec): 4005-15.

    Apoptosis regulates notochord development in Xenopus.
    Symes K
    Developmental biology 311.2 (2007 Nov 15): 434-48.

    Overexpression of XMyoD or XMyf5 in Xenopus embryos induces the formation of enlarged myotomes through recruitment of cells of nonsomitic lineage.
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    Diversification of the expression patterns and developmental functions of the dishevelled gene family during chordate evolution.
    Wallingford JB
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    EBF proteins participate in transcriptional regulation of Xenopus muscle development.
    Vetter ML
    Developmental biology 358.1 (2011 Oct 1): 240-50.

    Embryonic cells depleted of beta-catenin remain competent to differentiate into dorsal mesodermal derivatives.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 236.11 (2007 Nov): 3007-19.

    Temporal and spatial patterning of axial myotome fibers in Xenopus laevis.
    Domingo CR
    Developmental dynamics : an official publication of the American Association of Anatomists 239.4 (2010 Apr): 1162-77.

    WB References

    The translational repressor 4E-BP mediates hypoxia-induced defects in myotome cells.
    Darribère T
    Journal of cell science 125.Pt 17 (2012 Sep 1): 3989-4000.

    Monoclonal antibodies to the cells of a regenerating limb.
    Brockes JP
    Journal of embryology and experimental morphology 89. (1985 Oct): 37-55.

    beta-Catenin has Wnt-like activity and mimics the Nieuwkoop signaling center in Xenopus dorsal-ventral patterning.
    Gumbiner BM
    Developmental biology 172.1 (1995 Nov): 115-25.

    IHC References

    Phenotypic conversion of distinct muscle fiber populations to electrocytes in a weakly electric fish.
    Zakon HH
    The Journal of comparative neurology 399.1 (1998 Sep 14): 20-34.

    Daughters against dpp modulates dpp organizing activity in Drosophila wing development.
    Tabata T
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    Devoto SH
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    Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation.
    Weinstein DC
    Proceedings of the National Academy of Sciences of the United States of America 104.29 (2007 Jul 17): 12029-34.

    Requirement for Wnt and FGF signaling in Xenopus tadpole tail regeneration.
    Slack JM
    Developmental biology 316.2 (2008 Apr 15): 323-35.

    Control of muscle regeneration in the Xenopus tadpole tail by Pax7.
    Slack JM
    Development (Cambridge, England) 133.12 (2006 Jun): 2303-13.

    Rho guanine nucleotide exchange factor xNET1 implicated in gastrulation movements during Xenopus development.
    Kinoshita N
    Differentiation; research in biological diversity 72.1 (2004 Feb): 48-55.

    Role of cranial neural crest cells in visceral arch muscle positioning and morphogenesis in the Mexican axolotl, Ambystoma mexicanum.
    Olsson L
    Developmental dynamics : an official publication of the American Association of Anatomists 231.2 (2004 Oct): 237-47.

    Hes6 regulates myogenic differentiation.
    Jones PH
    Development (Cambridge, England) 129.9 (2002 May): 2195-207.

    BMP-4 is proteolytically activated by furin and/or PC6 during vertebrate embryonic development.
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    An examination of non-formalin-based fixation methods for Xenopus embryos.
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    Silencing of Smed-betacatenin1 generates radial-like hypercephalized planarians.
    Adell T
    Development (Cambridge, England) 135.7 (2008 Apr): 1215-21.

    Secondary coverage of the yolk by the body wall in the direct developing frog, Eleutherodactylus coqui: an unusual process for amphibian embryos.
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    XHas2 activity is required during somitogenesis and precursor cell migration in Xenopus development.
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    Xema, a foxi-class gene expressed in the gastrula stage Xenopus ectoderm, is required for the suppression of mesendoderm.
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    Development (Cambridge, England) 132.12 (2005 Jun): 2733-42.

    Direct activation of phospholipase C-gamma by fibroblast growth factor receptor is not required for mesoderm induction in Xenopus animal caps.
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    Thyroid hormone-dependent metamorphosis in a direct developing frog.
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    Lemaire P
    Developmental biology 260.2 (2003 Aug 15): 352-61.

    Identification and gene expression of versican during early development of Xenopus.
    Campo S
    The International journal of developmental biology 52.7 (2008): 993-8.

    Screening of FGF target genes in Xenopus by microarray: temporal dissection of the signalling pathway using a chemical inhibitor.
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    Function of the two Xenopus smad4s in early frog development.
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    Drosophila Tbx6-related gene, Dorsocross, mediates high levels of Dpp and Scw signal required for the development of amnioserosa and wing disc primordium.
    Murakami R
    Developmental biology 265.2 (2004 Jan 15): 355-68.

    XIPOU 2, a noggin-inducible gene, has direct neuralizing activity.
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    Planar induction of anteroposterior pattern in the developing central nervous system of Xenopus laevis.
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    Myoskeletin, a factor related to Myocardin, is expressed in somites and required for hypaxial muscle formation in Xenopus.
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    Inhibition of mesodermal fate by Xenopus HNF3beta/FoxA2.
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    Xenopus Tbx6 mediates posterior patterning via activation of Wnt and FGF signalling.
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    RGS proteins inhibit Xwnt-8 signaling in Xenopus embryonic development.
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    Comparison of the spectral biologically effective solar ultraviolet in adjacent tree shade and sun.
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    Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone.
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    The maternally localized RNA fatvg is required for cortical rotation and germ cell formation.
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    Induction of notochord cell intercalation behavior and differentiation by progressive signals in the gastrula of Xenopus laevis.
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    PKC delta is essential for Dishevelled function in a noncanonical Wnt pathway that regulates Xenopus convergent extension movements.
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    Low-molecular-weight protein tyrosine phosphatase is a positive component of the fibroblast growth factor receptor signaling pathway.
    Daar IO
    Molecular and cellular biology 22.10 (2002 May): 3404-14.

    Difference in the maternal and zygotic contributions of tumorhead on embryogenesis.
    Etkin LD
    Developmental biology 255.2 (2003 Mar 15): 290-302.

    Mef2d acts upstream of muscle identity genes and couples lateral myogenesis to dermomyotome formation in Xenopus laevis.
    Chanoine C
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    Function of the two Xenopus smad4s in early frog development.
    Harland RM
    The Journal of biological chemistry 281.41 (2006 Oct 13): 30794-803.

    Differential gene expression between the embryonic tail bud and regenerating larval tail in Xenopus laevis.
    Mochii M
    Development, growth and differentiation 46.1 (2004 Feb): 97-105.

    Hedgehog regulation of superficial slow muscle fibres in Xenopus and the evolution of tetrapod trunk myogenesis.
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    Lhx1 is required for specification of the renal progenitor cell field.
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    Docking protein SNT1 is a critical mediator of fibroblast growth factor signaling during Xenopus embryonic development.
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    Smad6 functions as an intracellular antagonist of some TGF-beta family members during Xenopus embryogenesis.
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    Paraxial T-box genes, Tbx6 and Tbx1, are required for cranial chondrogenesis and myogenesis.
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    The expression of XIF3 in undifferentiated anterior neuroectoderm, but not in primary neurons, is induced by the neuralizing agent noggin.
    Sharpe CR
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    Physical and functional interaction of murine and Xenopus Smad7 with bone morphogenetic protein receptors and transforming growth factor-beta receptors.
    ten Dijke P
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    DeltaNp63 antagonizes p53 to regulate mesoderm induction in Xenopus laevis.
    Pietenpol JA
    Developmental biology 329.1 (2009 May 1): 130-9.

    Jaw muscle development as evidence for embryonic repatterning in direct-developing frogs.
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    Essential role of MARCKS in cortical actin dynamics during gastrulation movements.
    Kinoshita N
    The Journal of cell biology 164.2 (2004 Jan 19): 169-74.

    Induction of anteroposterior neural pattern in Xenopus by planar signals.
    Doniach T
    Development (Cambridge, England). Supplement . (1992): 183-93.

    Xmab21l3 mediates dorsoventral patterning in Xenopus laevis.
    Weinstein DC
    Mechanisms of development 129.5-8 (2012 Jul): 136-46.

    Cranial muscle development in frogs with different developmental modes: direct development versus biphasic development.
    Diogo R
    Journal of morphology 275.4 (2014 Apr): 398-413.

    Xenopus Smad8 acts downstream of BMP-4 to modulate its activity during vertebrate embryonic patterning.
    Christian JL
    Development (Cambridge, England) 125.5 (1998 Mar): 857-67.

    Regulation of early Xenopus development by ErbB signaling.
    Chang C
    Developmental dynamics : an official publication of the American Association of Anatomists 235.2 (2006 Feb): 301-14.

    Early transcriptional targets of MyoD link myogenesis and somitogenesis.
    Pownall ME
    Developmental biology 371.2 (2012 Nov 15): 256-68.

    Subdividing the embryo: a role for Notch signaling during germ layer patterning in Xenopus laevis.
    McLaughlin KA
    Developmental biology 288.1 (2005 Dec 1): 294-307.

    Ectopic Hoxa2 induction after neural crest migration results in homeosis of jaw elements in Xenopus.
    Rijli FM
    Development (Cambridge, England) 127.24 (2000 Dec): 5367-78.

    β-Catenin-independent activation of TCF1/LEF1 in human hematopoietic tumor cells through interaction with ATF2 transcription factors.
    Aaronson SA
    PLoS genetics 9.8 (2013): e1003603.

    Pygopus is required for embryonic brain patterning in Xenopus.
    Kao KR
    Developmental biology 261.1 (2003 Sep 1): 132-48.

    Retinoid signaling can repress blastula Wnt signaling and impair dorsal development in Xenopus embryo.
    Ding X
    Differentiation; research in biological diversity 76.8 (2008 Oct): 897-907.

    Acute atrazine exposure disrupts matrix metalloproteinases and retinoid signaling during organ morphogenesis in Xenopus laevis.
    McLaughlin KA
    Journal of applied toxicology : JAT 30.6 (2010 Aug): 582-9.

    Post-transcriptional regulation of Xwnt-8 expression is required for normal myogenesis during vertebrate embryonic development.
    Christian JL
    Development (Cambridge, England) 126.15 (1999 Aug): 3371-80.

    Retinoic acid-dependent control of MAP kinase phosphatase-3 is necessary for early kidney development in Xenopus.
    Riou JF
    Biology of the cell 104.9 (2012 Sep): 516-32.

    Human truncated Smad 6 (Smad 6s) inhibits the BMP pathway in Xenopus laevis.
    Smith RC
    Development, growth and differentiation 43.2 (2001 Apr): 115-32.

    MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell plasticity and Xenopus embryonic cell fate.
    Boeuf H
    Development (Cambridge, England) 140.16 (2013 Aug): 3311-22.

    Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus.
    De Robertis EM
    Developmental biology 174.1 (1996 Feb 25): 174-8.

    Tumor cells induced by the v-src oncogene are heterogeneous for expression of markers of mesenchyme differentiation.
    Halpern MS
    Virchows Archiv : an international journal of pathology 424.1 (1994): 83-8.

    Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole.
    Mochii M
    BMC developmental biology 14. (2014 Jun 18): 27.

    FGF signalling in the early specification of mesoderm in Xenopus.
    Kirschner MW
    Development (Cambridge, England) 118.2 (1993 Jun): 477-87.

    Cell lineage tracing during Xenopus tail regeneration.
    Slack JM
    Development (Cambridge, England) 131.11 (2004 Jun): 2669-79.

    Mad is required for wingless signaling in wing development and segment patterning in Drosophila.
    De Robertis EM
    PloS one 4.8 (2009 Aug 6): e6543.

    Patterning the embryonic kidney: BMP signaling mediates the differentiation of the pronephric tubules and duct in Xenopus laevis.
    McLaughlin KA
    Developmental dynamics : an official publication of the American Association of Anatomists 237.1 (2008 Jan): 132-44.

    Regeneration of functional pronephric proximal tubules after partial nephrectomy in Xenopus laevis.
    Mclaughlin KA
    Developmental dynamics : an official publication of the American Association of Anatomists 242.3 (2013 Mar): 219-29.

    Cell motility driving mediolateral intercalation in explants of Xenopus laevis.
    Keller R
    Development (Cambridge, England) 116.4 (1992 Dec): 901-14.

    Muscular dystrophy candidate gene FRG1 is critical for muscle development.
    Jones PL
    Developmental dynamics : an official publication of the American Association of Anatomists 238.6 (2009 Jun): 1502-12.

    Spinal cord is required for proper regeneration of the tail in Xenopus tadpoles.
    Mochii M
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