P1D6

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$40.00
SKU: P1D6
View product citations for antibody P1D6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-5 (CD49e)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: P1D6 recognizes native protein only. This antibody works well with formalin, 4 % paraformadehyde, acetone-methanol or ice-cold methanol fixation.
Antigen Molecular Weight: 115 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGA5
Immunogen: Activated human killer T-lymphocytes (LAK)
Alternate Gene Names: CD49e, VLA5, FNRA
Alternate Antibody Name: CD49e
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: non-contiguous amino acids in N-terminal repeats of alpha 5
Uniprot ID: P08648 
Immunogen Sequence:
Entrez Gene ID: 3678 
Additional Characterization:  
Antibody Registry ID: AB_2619596 
Additional Information: P1D6 neutralizes binding of alpha5 beta 1 to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. RRID:AB_2619596
Recommended Applications: FFPE, Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1D6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    FB References

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.
    Gertler FB
    Cancer discovery 6.5 (2016 May): 516-31.

    MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis.
    Gertler FB
    Molecular biology of the cell 27.20 (2016 Oct 15): 3085-3094.

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
    Johnson PW
    The Journal of biological chemistry 274.43 (1999 Oct 22): 30906-13.

    Epitope Map References
    All References

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.
    Gertler FB
    Cancer discovery 6.5 (2016 May): 516-31.

    MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis.
    Gertler FB
    Molecular biology of the cell 27.20 (2016 Oct 15): 3085-3094.

    Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
    Johnson PW
    The Journal of biological chemistry 274.43 (1999 Oct 22): 30906-13.

    Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit.
    Humphries MJ
    The Journal of biological chemistry 272.28 (1997 Jul 11): 17283-92.

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