P1D6
Catalog Fields
Antigen: integrin alpha-5 (CD49e)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG3
Depositors Institution: Fred Hutchison Cancer Research Center,
Host Species: mouse
Depositors Notes: P1D6 recognizes native protein only. This antibody works well with formalin, 4 % paraformadehyde, acetone-methanol or ice-cold methanol fixation.
Positive Tested Species Reactivity: Human
Gene: ITGA5
Antigen Molecular Weight: 115 kDa
Alternate Gene Names: CD49e, VLA5, FNRA
Immunogen: Activated human killer T-lymphocytes (LAK)
Clonality: Monoclonal
Alternate Antibody Name: CD49e
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: non-contiguous amino acids in N-terminal repeats of alpha 5
Uniprot ID: P08648
Additional Information: P1D6 neutralizes binding of alpha5 beta 1 to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. RRID:AB_2619596
Entrez Gene ID: 3678
Antibody Registry ID: AB_2619596
Recommended Applications: FFPE, Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1D6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
8 References
Initial Publication
The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
Kunicki TJ
The Journal of cell biology 107.5 (1988 Nov): 1881-91.