P1D6

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$40.00
SKU: P1D6
View product citations for antibody P1D6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-5 (CD49e)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG3
Depositors Institution: Fred Hutchison Cancer Research Center,
Host Species: mouse
Depositors Notes: P1D6 recognizes native protein only. This antibody works well with formalin, 4 % paraformadehyde, acetone-methanol or ice-cold methanol fixation.
Positive Tested Species Reactivity: Human
Gene: ITGA5
Antigen Molecular Weight: 115 kDa
Alternate Gene Names: CD49e, VLA5, FNRA
Immunogen: Activated human killer T-lymphocytes (LAK)
Clonality: Monoclonal
Alternate Antibody Name: CD49e
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: non-contiguous amino acids in N-terminal repeats of alpha 5
Uniprot ID: P08648 
Additional Information: P1D6 neutralizes binding of alpha5 beta 1 to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. RRID:AB_2619596
Entrez Gene ID: 3678 
Antibody Registry ID: AB_2619596 
Recommended Applications: FFPE, Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1D6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    FB References

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.
    Gertler FB
    Cancer discovery 6.5 (2016 May): 516-31.

    MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis.
    Gertler FB
    Molecular biology of the cell 27.20 (2016 Oct 15): 3085-3094.

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
    Johnson PW
    The Journal of biological chemistry 274.43 (1999 Oct 22): 30906-13.

    Epitope Map References
    All References

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.
    Gertler FB
    Cancer discovery 6.5 (2016 May): 516-31.

    MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis.
    Gertler FB
    Molecular biology of the cell 27.20 (2016 Oct 15): 3085-3094.

    Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
    Johnson PW
    The Journal of biological chemistry 274.43 (1999 Oct 22): 30906-13.

    Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit.
    Humphries MJ
    The Journal of biological chemistry 272.28 (1997 Jul 11): 17283-92.

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