P1D6

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SKU: P1D6

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P1D6
Available to For-Profits: Yes
Alternate Antibody Name: CD49e
Gene Name: ITGA5
Ab Isotype: MIgG3
Gene Symbol:
Antibody Registry ID: AB_2619596 
Uniprot ID: P08648 
RRID:  
Entrez Gene ID: 3678 
Clonality: Monoclonal
Immunogen: Activated human killer T-lymphocytes (LAK)
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: integrin alpha-5 (CD49e)
Epitope Location or Sequence: non-contiguous amino acids in N-terminal repeats of alpha 5
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: 115 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: P1D6 recognizes native protein only. This antibody works well with formalin, 4 % paraformadehyde, acetone-methanol or ice-cold methanol fixation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: P1D6 neutralizes binding of alpha5 beta 1 to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. RRID:AB_2619596
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Function Blocking, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1D6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    FB References

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.
    Gertler FB
    Cancer discovery 6.5 (2016 May): 516-31.

    MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis.
    Gertler FB
    Molecular biology of the cell 27.20 (2016 Oct 15): 3085-3094.

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
    Johnson PW
    The Journal of biological chemistry 274.43 (1999 Oct 22): 30906-13.

    Epitope Map References
    All References

    Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
    Carter WG
    The Journal of cell biology 109.3 (1989 Sep): 1321-30.

    The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa.
    Kunicki TJ
    The Journal of cell biology 107.5 (1988 Nov): 1881-91.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression.
    Gertler FB
    Cancer discovery 6.5 (2016 May): 516-31.

    MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis.
    Gertler FB
    Molecular biology of the cell 27.20 (2016 Oct 15): 3085-3094.

    Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases.
    Johnson PW
    The Journal of biological chemistry 274.43 (1999 Oct 22): 30906-13.

    Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit.
    Humphries MJ
    The Journal of biological chemistry 272.28 (1997 Jul 11): 17283-92.

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