QH 1
Catalog Fields
Clone ID/Product Name: QH 1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_531829
Clonality: Monoclonal
Immunogen: Hematopoietic cells; H.H. Stage 13
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Quail endothelial cell surface
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/1/1987
Antigen Molecular Weight: Multiple bands between 35-250 kD
Depositor: Dieterlen-Lievre, F.
Antigen Sequence:
Depositor Institution: Institut d'Embryologie du CNRS
Antigen Species: Japanese quail
Depositor Notes: This antibody recognizes all quail cells in unfixed frozen sections but only vascular endothelial cells in paraffin sections in H.H. Stages 14 through 28.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Quail
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
QH 1 was deposited to the DSHB by Dieterlen-Lievre, F. (DSHB Hybridoma Product QH 1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
34 References
Initial Publication
Vasculogenesis in the early quail blastodisc as studied with a monoclonal antibody recognizing endothelial cells.
Buck CA
Development (Cambridge, England) 100.2 (1987 Jun): 339-49.