STRO-1
Catalog Fields
Antigen: Mesenchymal stem cell marker
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Torok-Storb, B.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse, Primate
Depositors Institution: Fred Hutchinson Research Center
Antigen Molecular Weight:
Depositors Notes: STRO-1 ihas recently been shown to recognize an endothelial antigen and is also expressed in many carcinoma cell lines. (PMID: 21903091).
Immunogen: CD34+ bone marrow cells
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1-Ag4-1
Epitope Mapped: No
Antibody Registry ID: AB_531913
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry
Additional Information: RRID:AB_531913
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
STRO-1 was deposited to the DSHB by Torok-Storb, B. (DSHB Hybridoma Product STRO-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
24 References
Initial Publication
Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1.
Torok-Storb B
Blood 78.1 (1991 Jul 1): 55-62.