STRO-1
Catalog Fields
Antigen: Mesenchymal stem cell marker
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Torok-Storb, B.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Research Center, Seattle, WA
Positive Tested Species Reactivity: Human, Mouse, Primate
Depositors Notes: STRO-1 ihas recently been shown to recognize an endothelial antigen and is also expressed in many carcinoma cell lines. (PMID: 21903091).
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Gene:
Immunogen: CD34+ bone marrow cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1-Ag4-1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531913
Additional Information: RRID:AB_531913
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
STRO-1 was deposited to the DSHB by Torok-Storb, B. (DSHB Hybridoma Product STRO-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
23 References
Initial Publication
Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1.
Torok-Storb B
Blood 78.1 (1991 Jul 1): 55-62.