5D2-27

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$40.00
SKU: 5D2-27-s
View product citations for antibody 5D2-27 on CiteAb

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Available: 136

DSHB Data Sheet

Catalog Fields

Antigen: CD44
Hybridoma Cells Available:
Antigen Species: Mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antigen was originally called Pgp-1 (Ly-24) lymphocyte cell adhesion glycoprotein and the antibody was identified as AMF-15. The 5D2-27 mAb is specific for mouse CD44 only.
Antigen Molecular Weight: 80kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CD44
Immunogen: NIH 3T3 fibroblast plasma membranes
Alternate Gene Names: Ly-24
Alternate Antibody Name: CD44
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3-NSI/1-Ag4-1
Epitope Location or Sequence:
Uniprot ID: P15379 
Immunogen Sequence: Full length protein
Entrez Gene ID: 12505 
Additional Characterization:
Antibody Registry ID: AB_2076440 
Additional Information: RRID:AB_2076440
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D2-27 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product 5D2-27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    IF References
    IHC References
    IP References

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    Redistribution of a major cell surface glycoprotein during cell movement.
    August JT
    The Journal of cell biology 99.5 (1984 Nov): 1613-23.

    Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells.
    August JT
    The Journal of biological chemistry 256.13 (1981 Jul 10): 7023-7.

    ELISA References

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    FACS References
    All References

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