5D2-27

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$40.00
SKU: 5D2-27-s
View product citations for antibody 5D2-27 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD44
Hybridoma Cells Available: No
Antigen Species: Mouse
Depositor: August, J.T.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antigen was originally called Pgp-1 (Ly-24) lymphocyte cell adhesion glycoprotein and the antibody was identified as AMF-15. The 5D2-27 mAb is specific for mouse CD44 only.
Antigen Molecular Weight: 80kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CD44
Immunogen: NIH 3T3 fibroblast plasma membranes
Alternate Gene Names: Ly-24
Alternate Antibody Name: CD44
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3-NSI/1-Ag4-1
Epitope Location or Sequence:
Uniprot ID: P15379 
Immunogen Sequence: Full length protein
Entrez Gene ID: 12505 
Additional Characterization:  
Antibody Registry ID: AB_2076440 
Additional Information: RRID:AB_2076440
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D2-27 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product 5D2-27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    IF References
    IHC References
    IP References

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    Redistribution of a major cell surface glycoprotein during cell movement.
    August JT
    The Journal of cell biology 99.5 (1984 Nov): 1613-23.

    Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells.
    August JT
    The Journal of biological chemistry 256.13 (1981 Jul 10): 7023-7.

    ELISA References

    Characterization of plasma membrane proteins identified by monoclonal antibodies.
    August JT
    The Journal of biological chemistry 256.2 (1981 Jan 25): 664-71.

    FACS References
    All References

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