40E-C

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SKU: 40E-C

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 40E-C
Available to For-Profits: Yes
Alternate Antibody Name: vimentin
Gene Name: VIM
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_528504 
Uniprot ID: XP_009084164.1 
RRID:  
Entrez Gene ID: 103812791 
Clonality: Monoclonal
Immunogen: Brain (adult canary); homogenized.
Clone:
Immunogen Sequence:
Myeloma Strain: P3U
Epitope Mapped: No
Antigen Name: vimentin; radial and radial glial cell marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/25/1994
Antigen Molecular Weight: Predicted: 55 kDa
Depositor: Alvarez-Buylla, A.
Antigen Sequence:
Depositor Institution: University of California, San Francisco, Neurological Surgery
Antigen Species: Canary brain
Depositor Notes: The sea cucumber Holothuria glaberrima stained positive for vimentin in radial glial cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Echinoderm, Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40E-C was deposited to the DSHB by Alvarez-Buylla, A. (DSHB Hybridoma Product 40E-C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

14 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Monoclonal antibody reveals radial glia in adult avian brain.
    Nottebohm F
    The Journal of comparative neurology 264.2 (1987 Oct 8): 159-70.

    IF References

    Identification of radial glia-like cells in the adult mouse olfactory bulb.
    Macklis JD
    Experimental neurology 236.2 (2012 Aug): 283-97.

    Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding.
    Han W
    eLife 5. (2016 Oct 18): .

    Distribution of the vasotocin type 4 receptor throughout the brain of the chicken, Gallus gallus.
    Kuenzel WJ
    The Journal of comparative neurology 523.2 (2015 Feb 1): 335-58.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Differential Fatty Acid-Binding Protein Expression in Persistent Radial Glia in the Human and Sheep Subventricular Zone.
    Curtis MA
    Developmental neuroscience 40.2 (2018): 145-161.

    Characterization of primary adult mouse cardiac fibroblast cultures.
    Pasumarthi KBS
    Canadian journal of physiology and pharmacology 98.12 (2020 Dec): 861-869.

    IHC References

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    Identification of radial glia-like cells in the adult mouse olfactory bulb.
    Macklis JD
    Experimental neurology 236.2 (2012 Aug): 283-97.

    Hypoxia stimulates hepatocyte epithelial to mesenchymal transition by hypoxia-inducible factor and transforming growth factor-beta-dependent mechanisms.
    Copple BL
    Liver international : official journal of the International Association for the Study of the Liver 30.5 (2010 May): 669-82.

    Comparative study of Pax2 expression in glial cells in the retina and optic nerve of birds and mammals.
    Fischer AJ
    The Journal of comparative neurology 518.12 (2010 Jun 15): 2316-33.

    Sex and age differences in brain-derived neurotrophic factor and vimentin in the zebra finch song system: Relationships to newly generated cells.
    Wade J
    The Journal of comparative neurology 524.5 (2016 Apr 1): 1081-96.

    Characterization of primary adult mouse cardiac fibroblast cultures.
    Pasumarthi KBS
    Canadian journal of physiology and pharmacology 98.12 (2020 Dec): 861-869.

    Stem cells distribution, cellular proliferation and migration in the adult Austrolebias charrua brain.
    Fernández AS
    Brain research 1673. (2017 Oct 15): 11-22.

    Coupling of GPR30 mediated neurogenesis and protection with astroglial Aromatase-STAT3 signaling in rat hippocampus after global cerebral ischemia.
    Wang R
    Molecular and cellular endocrinology 535. (2021 Sep 15): 111394.

    All References

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    Identification of radial glia-like cells in the adult mouse olfactory bulb.
    Macklis JD
    Experimental neurology 236.2 (2012 Aug): 283-97.

    Hypoxia stimulates hepatocyte epithelial to mesenchymal transition by hypoxia-inducible factor and transforming growth factor-beta-dependent mechanisms.
    Copple BL
    Liver international : official journal of the International Association for the Study of the Liver 30.5 (2010 May): 669-82.

    Comparative study of Pax2 expression in glial cells in the retina and optic nerve of birds and mammals.
    Fischer AJ
    The Journal of comparative neurology 518.12 (2010 Jun 15): 2316-33.

    Sex and age differences in brain-derived neurotrophic factor and vimentin in the zebra finch song system: Relationships to newly generated cells.
    Wade J
    The Journal of comparative neurology 524.5 (2016 Apr 1): 1081-96.

    Characterization of primary adult mouse cardiac fibroblast cultures.
    Pasumarthi KBS
    Canadian journal of physiology and pharmacology 98.12 (2020 Dec): 861-869.

    Stem cells distribution, cellular proliferation and migration in the adult Austrolebias charrua brain.
    Fernández AS
    Brain research 1673. (2017 Oct 15): 11-22.

    Coupling of GPR30 mediated neurogenesis and protection with astroglial Aromatase-STAT3 signaling in rat hippocampus after global cerebral ischemia.
    Wang R
    Molecular and cellular endocrinology 535. (2021 Sep 15): 111394.

    Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding.
    Han W
    eLife 5. (2016 Oct 18): .

    Distribution of the vasotocin type 4 receptor throughout the brain of the chicken, Gallus gallus.
    Kuenzel WJ
    The Journal of comparative neurology 523.2 (2015 Feb 1): 335-58.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Differential Fatty Acid-Binding Protein Expression in Persistent Radial Glia in the Human and Sheep Subventricular Zone.
    Curtis MA
    Developmental neuroscience 40.2 (2018): 145-161.

    Monoclonal antibody reveals radial glia in adult avian brain.
    Nottebohm F
    The Journal of comparative neurology 264.2 (1987 Oct 8): 159-70.

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