40E-C

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$40.00
SKU: 40E-C
View product citations for antibody 40E-C on CiteAb

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Available: 189

DSHB Data Sheet

Catalog Fields

Antigen: vimentin; radial and radial glial cell marker
Hybridoma Cells Available: Yes
Antigen Species: Canary brain
Depositor: Alvarez-Buylla, A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Neurological Surgery, University of California, San Francisco
Positive Tested Species Reactivity: Avian, Echinoderm, Human, Mouse, Rat
Depositors Notes: The sea cucumber Holothuria glaberrima stained positive for vimentin in radial glial cells.
Antigen Molecular Weight: Predicted: 55 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: VIM
Immunogen: Brain (adult canary); homogenized.
Alternate Gene Names:
Alternate Antibody Name: vimentin
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3U
Epitope Location or Sequence:
Uniprot ID: XP_009084164.1 
Immunogen Sequence:
Entrez Gene ID: 103812791 
Additional Characterization:
Antibody Registry ID: AB_528504 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40E-C was deposited to the DSHB by Alvarez-Buylla, A. (DSHB Hybridoma Product 40E-C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Monoclonal antibody reveals radial glia in adult avian brain.
    Nottebohm F
    The Journal of comparative neurology 264.2 (1987 Oct 8): 159-70.

    IF References
    IHC References

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    Identification of radial glia-like cells in the adult mouse olfactory bulb.
    Macklis JD
    Experimental neurology 236.2 (2012 Aug): 283-97.

    Hypoxia stimulates hepatocyte epithelial to mesenchymal transition by hypoxia-inducible factor and transforming growth factor-beta-dependent mechanisms.
    Copple BL
    Liver international : official journal of the International Association for the Study of the Liver 30.5 (2010 May): 669-82.

    Comparative study of Pax2 expression in glial cells in the retina and optic nerve of birds and mammals.
    Fischer AJ
    The Journal of comparative neurology 518.12 (2010 Jun 15): 2316-33.

    Sex and age differences in brain-derived neurotrophic factor and vimentin in the zebra finch song system: Relationships to newly generated cells.
    Wade J
    The Journal of comparative neurology 524.5 (2016 Apr 1): 1081-96.

    All References

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Characterization of a novel primary culture system of adult zebrafish brainstem cells.
    Plunkett JA
    Journal of neuroscience methods 223. (2014 Feb 15): 11-9.

    Identification of radial glia-like cells in the adult mouse olfactory bulb.
    Macklis JD
    Experimental neurology 236.2 (2012 Aug): 283-97.

    Hypoxia stimulates hepatocyte epithelial to mesenchymal transition by hypoxia-inducible factor and transforming growth factor-beta-dependent mechanisms.
    Copple BL
    Liver international : official journal of the International Association for the Study of the Liver 30.5 (2010 May): 669-82.

    Comparative study of Pax2 expression in glial cells in the retina and optic nerve of birds and mammals.
    Fischer AJ
    The Journal of comparative neurology 518.12 (2010 Jun 15): 2316-33.

    Sex and age differences in brain-derived neurotrophic factor and vimentin in the zebra finch song system: Relationships to newly generated cells.
    Wade J
    The Journal of comparative neurology 524.5 (2016 Apr 1): 1081-96.

    Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding.
    Han W
    eLife 5. (2016 Oct 18): .

    Distribution of the vasotocin type 4 receptor throughout the brain of the chicken, Gallus gallus.
    Kuenzel WJ
    The Journal of comparative neurology 523.2 (2015 Feb 1): 335-58.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Differential Fatty Acid-Binding Protein Expression in Persistent Radial Glia in the Human and Sheep Subventricular Zone.
    Curtis MA
    Developmental neuroscience 40.2 (2018): 145-161.

    Monoclonal antibody reveals radial glia in adult avian brain.
    Nottebohm F
    The Journal of comparative neurology 264.2 (1987 Oct 8): 159-70.

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