40E-C
Catalog Fields
Clone ID/Product Name: 40E-C
Available to For-Profits: Yes
Alternate Antibody Name: vimentin
Gene Symbol: VIM
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_528504
Uniprot ID: XP_009084164.1
Entrez Gene ID: 103812791
Clonality: Monoclonal
Immunogen: Brain (adult canary); homogenized.
Clone:
Immunogen Sequence:
Myeloma Strain: P3U
Epitope Mapped: No
Antigen Name: vimentin; radial and radial glial cell marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/25/1994
Antigen Molecular Weight: Predicted: 55 kDa
Depositor: Alvarez-Buylla, A.
Antigen Sequence:
Depositor Institution: University of California, San Francisco, Neurological Surgery
Antigen Species: Canary brain
Depositor Notes: The sea cucumber Holothuria glaberrima stained positive for vimentin in radial glial cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Echinoderm, Human, Mouse, Rat
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40E-C was deposited to the DSHB by Alvarez-Buylla, A. (DSHB Hybridoma Product 40E-C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
14 References
Initial Publication
Monoclonal antibody reveals radial glia in adult avian brain.
Nottebohm F
The Journal of comparative neurology 264.2 (1987 Oct 8): 159-70.