MNCD2

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$40.00
SKU: MNCD2
View product citations for antibody MNCD2 on CiteAb

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Available: 259

DSHB Data Sheet

Catalog Fields

Antigen: Cadherin, N- (neural)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Takeichi, M. / Matsunami, H.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Kyoto University
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: This antigen is also designated as CD325.
Antigen Molecular Weight: 99.76 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Cdh2
Immunogen: Recombinant protein domain
Alternate Gene Names: CDHN, N-CAD, Ncad
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3U1
Epitope Location or Sequence: Extracellular domain of N-cadherin
Uniprot ID: P15116 
Immunogen Sequence: a.a. 308-597
Entrez Gene ID: 12558 
Additional Characterization:
Antibody Registry ID: AB_528119 
Additional Information: RRID:AB_528119
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MNCD2 was deposited to the DSHB by Takeichi, M. / Matsunami, H. (DSHB Hybridoma Product MNCD2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Selective Connexin43 Inhibition Prevents Isoproterenol-Induced Arrhythmias and Lethality in Muscular Dystrophy Mice.
    Fraidenraich D
    Scientific reports 5. (2015 Aug 27): 13490.

    In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity.
    Mayor R
    The Journal of cell biology 206.1 (2014 Jul 7): 113-27.

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    MMP14 Regulates Cranial Neural Crest Epithelial-to-Mesenchymal Transition and Migration.
    Nie S
    Developmental dynamics : an official publication of the American Association of Anatomists 247.9 (2018 Sep): 1083-1092.

    WB References

    Fetal brain subdivisions defined by R- and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion.
    Takeichi M
    Developmental biology 172.2 (1995 Dec): 466-78.

    Knock-down of ZBED6 in insulin-producing cells promotes N-cadherin junctions between beta-cells and neural crest stem cells in vitro.
    Welsh N
    Scientific reports 6. (2016 Jan 11): 19006.

    N-cadherin promotes recruitment and migration of neural progenitor cells from the SVZ neural stem cell niche into demyelinated lesions.
    Aguirre A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.29 (2014 Jul 16): 9590-606.

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    Functional cis-heterodimers of N- and R-cadherins.
    Shapiro L
    The Journal of cell biology 148.3 (2000 Feb 7): 579-90.

    IHC References
    IP References

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    Functional cis-heterodimers of N- and R-cadherins.
    Shapiro L
    The Journal of cell biology 148.3 (2000 Feb 7): 579-90.

    Plasticity of cadherin-catenin expression in the melanocyte lineage.
    Larue L
    Pigment cell research 13.4 (2000 Aug): 260-72.

    Epitope Map References
    All References

    Fetal brain subdivisions defined by R- and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion.
    Takeichi M
    Developmental biology 172.2 (1995 Dec): 466-78.

    Canonical Wnt/β-catenin signalling is essential for optic cup formation.
    Carlsson L
    PloS one 8.12 (2013): e81158.

    Mouse Tenm4 is required for mesoderm induction.
    Justice MJ
    BMC developmental biology 13. (2013 Mar 25): 9.

    Selective Connexin43 Inhibition Prevents Isoproterenol-Induced Arrhythmias and Lethality in Muscular Dystrophy Mice.
    Fraidenraich D
    Scientific reports 5. (2015 Aug 27): 13490.

    In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity.
    Mayor R
    The Journal of cell biology 206.1 (2014 Jul 7): 113-27.

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    MMP14 Regulates Cranial Neural Crest Epithelial-to-Mesenchymal Transition and Migration.
    Nie S
    Developmental dynamics : an official publication of the American Association of Anatomists 247.9 (2018 Sep): 1083-1092.

    Knock-down of ZBED6 in insulin-producing cells promotes N-cadherin junctions between beta-cells and neural crest stem cells in vitro.
    Welsh N
    Scientific reports 6. (2016 Jan 11): 19006.

    N-cadherin promotes recruitment and migration of neural progenitor cells from the SVZ neural stem cell niche into demyelinated lesions.
    Aguirre A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.29 (2014 Jul 16): 9590-606.

    Functional cis-heterodimers of N- and R-cadherins.
    Shapiro L
    The Journal of cell biology 148.3 (2000 Feb 7): 579-90.

    Plasticity of cadherin-catenin expression in the melanocyte lineage.
    Larue L
    Pigment cell research 13.4 (2000 Aug): 260-72.

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