MNCD2

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$40.00
SKU: MNCD2
View product citations for antibody MNCD2 on CiteAb

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Available: 163

DSHB Data Sheet

Catalog Fields

Antigen: Cadherin, N- (neural)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Takeichi, M. / Matsunami, H.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Kyoto University
Positive Tested Species Reactivity: Chick, Human, Mouse
Depositors Notes: This antigen is also designated as CD325.
Antigen Molecular Weight: 99.76 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Cdh2
Immunogen: Recombinant protein domain
Alternate Gene Names: CDHN, N-CAD, Ncad
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3U1
Epitope Location or Sequence: Extracellular domain of N-cadherin
Uniprot ID: P15116 
Immunogen Sequence: a.a. 308-597
Entrez Gene ID: 12558 
Additional Characterization:  
Antibody Registry ID: AB_528119 
Additional Information: RRID:AB_528119
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MNCD2 was deposited to the DSHB by Takeichi, M. / Matsunami, H. (DSHB Hybridoma Product MNCD2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Selective Connexin43 Inhibition Prevents Isoproterenol-Induced Arrhythmias and Lethality in Muscular Dystrophy Mice.
    Fraidenraich D
    Scientific reports 5. (2015 Aug 27): 13490.

    In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity.
    Mayor R
    The Journal of cell biology 206.1 (2014 Jul 7): 113-27.

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    MMP14 Regulates Cranial Neural Crest Epithelial-to-Mesenchymal Transition and Migration.
    Nie S
    Developmental dynamics : an official publication of the American Association of Anatomists 247.9 (2018 Sep): 1083-1092.

    WB References

    Fetal brain subdivisions defined by R- and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion.
    Takeichi M
    Developmental biology 172.2 (1995 Dec): 466-78.

    Knock-down of ZBED6 in insulin-producing cells promotes N-cadherin junctions between beta-cells and neural crest stem cells in vitro.
    Welsh N
    Scientific reports 6. (2016 Jan 11): 19006.

    N-cadherin promotes recruitment and migration of neural progenitor cells from the SVZ neural stem cell niche into demyelinated lesions.
    Aguirre A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.29 (2014 Jul 16): 9590-606.

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    Functional cis-heterodimers of N- and R-cadherins.
    Shapiro L
    The Journal of cell biology 148.3 (2000 Feb 7): 579-90.

    IHC References
    IP References

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    Functional cis-heterodimers of N- and R-cadherins.
    Shapiro L
    The Journal of cell biology 148.3 (2000 Feb 7): 579-90.

    Plasticity of cadherin-catenin expression in the melanocyte lineage.
    Larue L
    Pigment cell research 13.4 (2000 Aug): 260-72.

    Epitope Map References
    All References

    Fetal brain subdivisions defined by R- and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion.
    Takeichi M
    Developmental biology 172.2 (1995 Dec): 466-78.

    Canonical Wnt/β-catenin signalling is essential for optic cup formation.
    Carlsson L
    PloS one 8.12 (2013): e81158.

    Mouse Tenm4 is required for mesoderm induction.
    Justice MJ
    BMC developmental biology 13. (2013 Mar 25): 9.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Early expression of Tubulin Beta-III in avian cranial neural crest cells.
    Rogers CD
    Gene expression patterns : GEP 34. (2019 Dec): 119067.

    Data on the effects of N-cadherin perturbation on the expression of type II cadherin proteins and major signaling pathways.
    Rogers CD
    Data in brief 20. (2018 Oct): 419-425.

    A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.
    Bronner ME
    Mechanisms of development 152. (2018 Aug): 44-56.

    DWnt4 and DWnt10 Regulate Morphogenesis and Arrangement of Columnar Units via Fz2/PCP Signaling in the Drosophila Brain.
    Sato M
    Cell reports 33.4 (2020 Oct 27): 108305.

    Selective Connexin43 Inhibition Prevents Isoproterenol-Induced Arrhythmias and Lethality in Muscular Dystrophy Mice.
    Fraidenraich D
    Scientific reports 5. (2015 Aug 27): 13490.

    In vivo collective cell migration requires an LPAR2-dependent increase in tissue fluidity.
    Mayor R
    The Journal of cell biology 206.1 (2014 Jul 7): 113-27.

    Cadherins regulate aggregation of pancreatic beta-cells in vivo.
    Semb H
    Development (Cambridge, England) 122.9 (1996 Sep): 2895-902.

    MMP14 Regulates Cranial Neural Crest Epithelial-to-Mesenchymal Transition and Migration.
    Nie S
    Developmental dynamics : an official publication of the American Association of Anatomists 247.9 (2018 Sep): 1083-1092.

    Knock-down of ZBED6 in insulin-producing cells promotes N-cadherin junctions between beta-cells and neural crest stem cells in vitro.
    Welsh N
    Scientific reports 6. (2016 Jan 11): 19006.

    N-cadherin promotes recruitment and migration of neural progenitor cells from the SVZ neural stem cell niche into demyelinated lesions.
    Aguirre A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.29 (2014 Jul 16): 9590-606.

    Functional cis-heterodimers of N- and R-cadherins.
    Shapiro L
    The Journal of cell biology 148.3 (2000 Feb 7): 579-90.

    Plasticity of cadherin-catenin expression in the melanocyte lineage.
    Larue L
    Pigment cell research 13.4 (2000 Aug): 260-72.

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