rat-401

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$40.00
SKU: rat-401
View product citations for antibody rat-401 on CiteAb

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Available: 113

DSHB Data Sheet

Catalog Fields

Antigen: Nestin (intermediate filament protein)
Hybridoma Cells Available:
Antigen Species: Rat
Depositor: Hockfield, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University School of Medicine
Positive Tested Species Reactivity: Avian, Feline, Human, Mouse, Primate, Rat
Depositors Notes: Nestin is expressed by progenitor glial cells in the CNS, Schwann cells in the PNS and early myoblast/myotubes.
Antigen Molecular Weight: 208.7 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Nes
Immunogen: Spinal cord homogenate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P21263 
Immunogen Sequence:
Entrez Gene ID: 25491 
Additional Characterization:
Antibody Registry ID: AB_2235915 
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rat-401 was deposited to the DSHB by Hockfield, S. (DSHB Hybridoma Product rat-401)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

30 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication

    Identification of major cell classes in the developing mammalian nervous system.
    McKay RD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 5.12 (1985 Dec): 3310-28.

    IF References

    Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.
    Wolfe JH
    Stem cells translational medicine 2.6 (2013 Jun): 444-54.

    Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers.
    Leonhardt H
    Nucleic acids research 40.12 (2012 Jul): 5368-77.

    All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neurons.
    Martinez-Ceballos E
    Differentiation; research in biological diversity 83.5 (2012 Jun): 233-41.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    Efficient differentiation of embryonic stem cells into mesodermal precursors by BMP, retinoic acid and Notch signalling.
    Watt FM
    PloS one 7.4 (2012): e36405.

    Uni-directional differentiation of mouse embryonic stem cells into neurons by the neural stem sphere method.
    Inoue N
    Neuroscience research 69.4 (2011 Apr): 314-21.

    The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature.
    Okano H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 58.8 (2010 Aug): 721-30.

    The nuclear periphery of embryonic stem cells is a transcriptionally permissive and repressive compartment.
    Shopland LS
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3729-37.

    Identification of neural crest-derived stem cell-like cells from the corneal limbus of juvenile mice.
    Morsczeck C
    Experimental eye research 89.2 (2009 Aug): 209-17.

    Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Brüstle O
    Stem cells (Dayton, Ohio) 25.4 (2007 Apr): 939-49.

    Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
    Doetsch F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.11 (2015 Mar 18): 4528-39.

    Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status.
    Mather ML
    Scientific reports 8.1 (2018 Jan 15): 697.

    Aligned contiguous microfiber platform enhances neural differentiation of embryonic stem cells.
    Hu Z
    Scientific reports 8.1 (2018 Apr 17): 6087.

    Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive Pluripotency.
    Huber W
    Cell systems 7.5 (2018 Nov 28): 482-495.e10.

    WB References
    IHC References

    Enteric nervous system abnormalities are present in human necrotizing enterocolitis: potential neurotransplantation therapy.
    Besner GE
    Stem cell research and therapy 4.6 (2013): 157.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    N-cadherin regulates the proliferation and differentiation of ventral midbrain dopaminergic progenitors.
    Miyamoto Y
    Developmental neurobiology 73.7 (2013 Jul): 518-29.

    Dynamics of 5-hydroxymethylcytosine and chromatin marks in Mammalian neurogenesis.
    Lu Q
    Cell reports 3.2 (2013 Feb 21): 291-300.

    Dicer is required for proliferation, viability, migration and differentiation in corticoneurogenesis.
    Davidson BL
    Neuroscience 223. (2012 Oct 25): 285-95.

    Functional dicer is necessary for appropriate specification of radial glia during early development of mouse telencephalon.
    Price DJ
    PloS one 6.8 (2011): e23013.

    Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon-mediated gene transfer system.
    Miura M
    Genes to cells : devoted to molecular and cellular mechanisms 15.5 (2010 May): 501-12.

    Delayed dopaminergic neuron differentiation in Lrp6 mutant mice.
    Arenas E
    Developmental dynamics : an official publication of the American Association of Anatomists 239.1 (2010 Jan): 211-21.

    Efficient gene targeting by homologous recombination in rat embryonic stem cells.
    Burdon T
    PloS one 5.12 (2010 Dec 3): e14225.

    Promotion of proliferation in the developing cerebral cortex by EphA4 forward signaling.
    Donoghue MJ
    Development (Cambridge, England) 136.14 (2009 Jul): 2467-76.

    Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice.
    Parnavelas JG
    The Journal of comparative neurology 524.3 (2016 Feb 15): 518-34.

    ELISA References

    Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis.
    Lopez-Toledano MA
    International journal of Alzheimer's disease 2011. (2011): 929042.

    FACS References
    All References

    Enteric nervous system abnormalities are present in human necrotizing enterocolitis: potential neurotransplantation therapy.
    Besner GE
    Stem cell research and therapy 4.6 (2013): 157.

    Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation.
    Sheen V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.40 (2013 Oct 2): 15735-46.

    N-cadherin regulates the proliferation and differentiation of ventral midbrain dopaminergic progenitors.
    Miyamoto Y
    Developmental neurobiology 73.7 (2013 Jul): 518-29.

    Dynamics of 5-hydroxymethylcytosine and chromatin marks in Mammalian neurogenesis.
    Lu Q
    Cell reports 3.2 (2013 Feb 21): 291-300.

    Dicer is required for proliferation, viability, migration and differentiation in corticoneurogenesis.
    Davidson BL
    Neuroscience 223. (2012 Oct 25): 285-95.

    Functional dicer is necessary for appropriate specification of radial glia during early development of mouse telencephalon.
    Price DJ
    PloS one 6.8 (2011): e23013.

    Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon-mediated gene transfer system.
    Miura M
    Genes to cells : devoted to molecular and cellular mechanisms 15.5 (2010 May): 501-12.

    Delayed dopaminergic neuron differentiation in Lrp6 mutant mice.
    Arenas E
    Developmental dynamics : an official publication of the American Association of Anatomists 239.1 (2010 Jan): 211-21.

    Efficient gene targeting by homologous recombination in rat embryonic stem cells.
    Burdon T
    PloS one 5.12 (2010 Dec 3): e14225.

    Promotion of proliferation in the developing cerebral cortex by EphA4 forward signaling.
    Donoghue MJ
    Development (Cambridge, England) 136.14 (2009 Jul): 2467-76.

    Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice.
    Parnavelas JG
    The Journal of comparative neurology 524.3 (2016 Feb 15): 518-34.

    Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage.
    Wolfe JH
    Stem cells translational medicine 2.6 (2013 Jun): 444-54.

    Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers.
    Leonhardt H
    Nucleic acids research 40.12 (2012 Jul): 5368-77.

    All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neurons.
    Martinez-Ceballos E
    Differentiation; research in biological diversity 83.5 (2012 Jun): 233-41.

    Multiple phenotypes in Huntington disease mouse neural stem cells.
    Kegel KB
    Molecular and cellular neurosciences 50.1 (2012 May): 70-81.

    Efficient differentiation of embryonic stem cells into mesodermal precursors by BMP, retinoic acid and Notch signalling.
    Watt FM
    PloS one 7.4 (2012): e36405.

    Uni-directional differentiation of mouse embryonic stem cells into neurons by the neural stem sphere method.
    Inoue N
    Neuroscience research 69.4 (2011 Apr): 314-21.

    The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature.
    Okano H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 58.8 (2010 Aug): 721-30.

    The nuclear periphery of embryonic stem cells is a transcriptionally permissive and repressive compartment.
    Shopland LS
    Journal of cell science 122.Pt 20 (2009 Oct 15): 3729-37.

    Identification of neural crest-derived stem cell-like cells from the corneal limbus of juvenile mice.
    Morsczeck C
    Experimental eye research 89.2 (2009 Aug): 209-17.

    Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Brüstle O
    Stem cells (Dayton, Ohio) 25.4 (2007 Apr): 939-49.

    Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
    Doetsch F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.11 (2015 Mar 18): 4528-39.

    Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status.
    Mather ML
    Scientific reports 8.1 (2018 Jan 15): 697.

    Aligned contiguous microfiber platform enhances neural differentiation of embryonic stem cells.
    Hu Z
    Scientific reports 8.1 (2018 Apr 17): 6087.

    Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive Pluripotency.
    Huber W
    Cell systems 7.5 (2018 Nov 28): 482-495.e10.

    Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis.
    Lopez-Toledano MA
    International journal of Alzheimer's disease 2011. (2011): 929042.

    Normal distribution and medullary-to-cortical shift of Nestin-expressing cells in acute renal ischemia.
    Goligorsky MS
    Kidney international 71.8 (2007 Apr): 744-54.

    EphA receptors direct the differentiation of mammalian neural precursor cells through a mitogen-activated protein kinase-dependent pathway.
    Tohyama M
    The Journal of biological chemistry 279.31 (2004 Jul 30): 32643-50.

    Notch exhibits ligand bias and maneuvers stage-specific steering of neural differentiation in embryonic stem cells.
    Lenka N
    Molecular and cellular biology 30.8 (2010 Apr): 1946-57.

    Identification of major cell classes in the developing mammalian nervous system.
    McKay RD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 5.12 (1985 Dec): 3310-28.

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