6B6

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$40.00
SKU: 6B6
View product citations for antibody 6B6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin, C- (3)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Gumbiner, B.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Xenopus
Depositors Notes: This antibody blocks aggregation of embryonic blastomeres.
Antigen Molecular Weight: 120 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cdh3.L
Immunogen: EC2 extracellular domain - GST fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP-2/0
Epitope Location or Sequence:
Uniprot ID: P33148 
Immunogen Sequence: Extracellular domain
Entrez Gene ID: 594865 
Additional Characterization:  
Antibody Registry ID: AB_528113 
Additional Information: RRID: AB_528113
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B6 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 6B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FB References
  • All References
  • Initial Publication

    Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
    Gumbiner BM
    The Journal of cell biology 126.2 (1994 Jul): 519-27.

    IF References

    EphA7 modulates apical constriction of hindbrain neuroepithelium during neurulation in Xenopus.
    Mao B
    Biochemical and biophysical research communications 479.4 (2016 Oct 28): 759-765.

    Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
    Gumbiner BM
    The Journal of cell biology 126.2 (1994 Jul): 519-27.

    Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos.
    Tramier M
    Scientific reports 7. (2017 Mar 22): 45058.

    Spatial regulation of cell cohesion by Wnt5a during second heart field progenitor deployment.
    Wang J
    Developmental biology 412.1 (2016 Apr 1): 18-31.

    Par6b regulates the dynamics of apicobasal polarity during development of the stratified Xenopus epidermis.
    Wylie C
    PloS one 8.10 (2013): e76854.

    Cell-cycle dependent localization of MELK and its new partner RACK1 in epithelial versus mesenchyme-like cells in Xenopus embryo.
    Tassan JP
    Biology open 2.10 (2013): 1037-48.

    Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin.
    Wedlich D
    The Journal of cell biology 198.4 (2012 Aug 20): 695-709.

    Regulation of classical cadherin membrane expression and F-actin assembly by alpha-catenins, during Xenopus embryogenesis.
    Wylie C
    PloS one 7.6 (2012): e38756.

    Roles for Xenopus aquaporin-3b (aqp3.L) during gastrulation: Fibrillar fibronectin and tissue boundary establishment in the dorsal margin.
    Merzdorf CS
    Developmental biology 433.1 (2018 Jan 1): 3-16.

    E-cadherin is required for cranial neural crest migration in Xenopus laevis.
    Kashef J
    Developmental biology 411.2 (2016 Mar 15): 159-171.

    WB References
    IHC References

    Folate receptor 1 is necessary for neural plate cell apical constriction during <i>Xenopus</i> neural tube formation.
    Borodinsky LN
    Development (Cambridge, England) 144.8 (2017 Apr 15): 1518-1530.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    Molecular model for force production and transmission during vertebrate gastrulation.
    Skoglund P
    Development (Cambridge, England) 143.4 (2016 Feb 15): 715-27.

    Different thresholds of Wnt-Frizzled 7 signaling coordinate proliferation, morphogenesis and fate of endoderm progenitor cells.
    Zorn AM
    Developmental biology 378.1 (2013 Jun 1): 1-12.

    Cadherin-dependent differential cell adhesion in Xenopus causes cell sorting in vitro but not in the embryo.
    Winklbauer R
    Journal of cell science 125.Pt 8 (2012 Apr 15): 1877-83.

    Large-scale mechanical properties of Xenopus embryonic epithelium.
    Winklbauer R
    Proceedings of the National Academy of Sciences of the United States of America 108.10 (2011 Mar 8): 4000-5.

    MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.
    Ueno N
    Development (Cambridge, England) 137.14 (2010 Jul): 2329-39.

    Sfrp5 coordinates foregut specification and morphogenesis by antagonizing both canonical and noncanonical Wnt11 signaling.
    Zorn AM
    Genes & development 22.21 (2008 Nov 1): 3050-63.

    IP References

    Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
    Gumbiner BM
    The Journal of cell biology 126.2 (1994 Jul): 519-27.

    FFPE References

    EphA7 modulates apical constriction of hindbrain neuroepithelium during neurulation in Xenopus.
    Mao B
    Biochemical and biophysical research communications 479.4 (2016 Oct 28): 759-765.

    Folate receptor 1 is necessary for neural plate cell apical constriction during <i>Xenopus</i> neural tube formation.
    Borodinsky LN
    Development (Cambridge, England) 144.8 (2017 Apr 15): 1518-1530.

    FB References

    Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
    Gumbiner BM
    The Journal of cell biology 126.2 (1994 Jul): 519-27.

    All References

    Folate receptor 1 is necessary for neural plate cell apical constriction during <i>Xenopus</i> neural tube formation.
    Borodinsky LN
    Development (Cambridge, England) 144.8 (2017 Apr 15): 1518-1530.

    Syndecan4 coordinates Wnt/JNK and BMP signaling to regulate foregut progenitor development.
    Zorn AM
    Developmental biology 416.1 (2016 Aug 1): 187-199.

    Molecular model for force production and transmission during vertebrate gastrulation.
    Skoglund P
    Development (Cambridge, England) 143.4 (2016 Feb 15): 715-27.

    Different thresholds of Wnt-Frizzled 7 signaling coordinate proliferation, morphogenesis and fate of endoderm progenitor cells.
    Zorn AM
    Developmental biology 378.1 (2013 Jun 1): 1-12.

    Cadherin-dependent differential cell adhesion in Xenopus causes cell sorting in vitro but not in the embryo.
    Winklbauer R
    Journal of cell science 125.Pt 8 (2012 Apr 15): 1877-83.

    Large-scale mechanical properties of Xenopus embryonic epithelium.
    Winklbauer R
    Proceedings of the National Academy of Sciences of the United States of America 108.10 (2011 Mar 8): 4000-5.

    MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.
    Ueno N
    Development (Cambridge, England) 137.14 (2010 Jul): 2329-39.

    Sfrp5 coordinates foregut specification and morphogenesis by antagonizing both canonical and noncanonical Wnt11 signaling.
    Zorn AM
    Genes & development 22.21 (2008 Nov 1): 3050-63.

    EphA7 modulates apical constriction of hindbrain neuroepithelium during neurulation in Xenopus.
    Mao B
    Biochemical and biophysical research communications 479.4 (2016 Oct 28): 759-765.

    Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
    Gumbiner BM
    The Journal of cell biology 126.2 (1994 Jul): 519-27.

    Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos.
    Tramier M
    Scientific reports 7. (2017 Mar 22): 45058.

    Spatial regulation of cell cohesion by Wnt5a during second heart field progenitor deployment.
    Wang J
    Developmental biology 412.1 (2016 Apr 1): 18-31.

    Par6b regulates the dynamics of apicobasal polarity during development of the stratified Xenopus epidermis.
    Wylie C
    PloS one 8.10 (2013): e76854.

    Cell-cycle dependent localization of MELK and its new partner RACK1 in epithelial versus mesenchyme-like cells in Xenopus embryo.
    Tassan JP
    Biology open 2.10 (2013): 1037-48.

    Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin.
    Wedlich D
    The Journal of cell biology 198.4 (2012 Aug 20): 695-709.

    Regulation of classical cadherin membrane expression and F-actin assembly by alpha-catenins, during Xenopus embryogenesis.
    Wylie C
    PloS one 7.6 (2012): e38756.

    Roles for Xenopus aquaporin-3b (aqp3.L) during gastrulation: Fibrillar fibronectin and tissue boundary establishment in the dorsal margin.
    Merzdorf CS
    Developmental biology 433.1 (2018 Jan 1): 3-16.

    E-cadherin is required for cranial neural crest migration in Xenopus laevis.
    Kashef J
    Developmental biology 411.2 (2016 Mar 15): 159-171.

    G protein-coupled receptors Flop1 and Flop2 inhibit Wnt/β-catenin signaling and are essential for head formation in Xenopus.
    Ueno N
    Developmental biology 407.1 (2015 Nov 1): 131-44.

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