Antigen: cadherin, C- (3)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Gumbiner, B.M.
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Xenopus
Depositors Notes: This antibody blocks aggregation of embryonic blastomeres.
Antigen Molecular Weight: 120 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: EC2 extracellular domain - GST fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: SP-2/0
Epitope Location or Sequence:
Uniprot ID: P33148
Immunogen Sequence: Extracellular domain
Entrez Gene ID: 594865
Antibody Registry ID: AB_528113
Additional Information: RRID: AB_528113
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B6 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 6B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).