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SKU: 8C2

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 8C2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: cdh1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528117 
Uniprot ID: P30944 
Entrez Gene ID: 100337618 
Clonality: Monoclonal
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Immunogen Sequence: partial protein
Myeloma Strain: sp-2/0
Epitope Mapped: Yes
Antigen Name: cadherin, E-
Epitope Location or Sequence: Extracellular domain
Alternate Antigen Name:
Deposit Date: 5/7/1993
Antigen Molecular Weight: Predicted: 79 kDa (mature E-cadherin); Apparent: 140 kDa
Depositor: Gumbiner, B.M.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Xenopus
Depositor Notes: 8C2 only binds the -(Ca++) conformation of E-cadherin. Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian, Xenopus
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8C2 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 8C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FB References
    Epitope Map References
    All References

    A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.
    Bronner ME
    Mechanisms of development 152. (2018 Aug): 44-56.

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    The distribution of E-cadherin during Xenopus laevis development.
    Thiery JP
    Development (Cambridge, England) 111.1 (1991 Jan): 159-69.

    Pancreatic alpha-cell differentiation by mesenchyme-to-epithelial transition: implications for cell-based therapies in children.
    Johnson PR
    Journal of pediatric surgery 42.1 (2007 Jan): 153-9.

    Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model.
    Johnson PR
    Pediatric surgery international 21.3 (2005 Mar): 138-42.

    Foregut Mesenchyme Contributes Cells to Islets during Pancreatic Development in a 3-Dimensional Avian Model.
    Johnson PR
    Organogenesis 1.2 (2004 Oct): 45-51.

    The competency of foregut mesenchyme in islet mesenchyme-to-epithelial transition during embryonic development.
    Johnson PR
    Journal of pediatric surgery 41.2 (2006 Feb): 347-51.

    Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.
    Asashima M
    Advances in space research : the official journal of the Committee on Space Research (COSPAR) 35.9 (2005): 1654-61.

    Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
    Gumbiner BM
    Development (Cambridge, England) 120.4 (1994 Apr): 901-9.

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