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SKU: 8C2
View product citations for antibody 8C2 on CiteAb

In Stock

Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: cadherin, E-
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Gumbiner, B.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cell Biology, School of Medicine, University of Virginia
Positive Tested Species Reactivity: Avian, Xenopus
Depositors Notes: 8C2 only binds the -(Ca++) conformation of E-cadherin. Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Antigen Molecular Weight: Predicted: 79 kDa (mature E-cadherin); Apparent: 140 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: cdh1
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Alternate Gene Names: uvo, cdh1, cdhe, ecad, lcam, arc-1, cd324
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: sp-2/0
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P30944 
Immunogen Sequence: partial protein
Entrez Gene ID: 100337618 
Additional Characterization:
Antibody Registry ID: AB_528117 
Additional Information:
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8C2 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 8C2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References
    Epitope Map References
    All References

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