Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Positive Tested Species Reactivity: C. elegans
Depositors Institution: Washington University Medical School
Antigen Molecular Weight: Predicted: 104 kDa; Apparent: 102 and 250 kDa
Depositors Notes: Works on C. elegans whole mounts. Works poorly on immunoblots.
Immunogen: Recombinant His6-tagged APA-2 (C-terminal 308 aa) fusion protein
Alternate Antibody Name: 11B11
Alternate Gene Names:
Alternate Antigen Name:
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Uniprot ID: Q22601
Epitope Location or Sequence: C-terminal 308 aa
Entrez Gene ID: 181163
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_10567130
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: The AdaPtin, Alpha chain (Clathrin associated complex), APA-2, is an adaptin for the alpha subunit of the adaptor protein complex 2 (AP2) that is involved in endocytosis.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
APA2 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product APA2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Epitope Map References