APA2

(0)No Reviews yet
$50.00 to $600.00
SKU: APA2

In Stock

Available: 15

DSHB Data Sheet

Catalog Fields

Product Name/ID: APA2
Available to For-Profits: Yes
Alternate Antibody Name: 11B11
Gene Name: apa-2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_10567130 
Uniprot ID: Q22601 
RRID:  
Entrez Gene ID: 181163 
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged APA-2 (C-terminal 308 aa) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: Adaptin
Epitope Location or Sequence: C-terminal 308 aa
Alternate Antigen Name:
Deposit Date: 2/16/2010
Antigen Molecular Weight: Predicted: 104 kDa; Apparent: 102 and 250 kDa
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Antigen Sequence:
Depositor Institution: Washington University Medical School
Antigen Species: C. elegans
Depositor Notes: Works on C. elegans whole mounts. Works poorly on immunoblots.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: The AdaPtin, Alpha chain (Clathrin associated complex), APA-2, is an adaptin for the alpha subunit of the adaptor protein complex 2 (AP2) that is involved in endocytosis.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
APA2 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product APA2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review