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View product citations for antibody CAV1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Caveolin
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works well on C. elegans whole mounts. Stains caveolae.
Antigen Molecular Weight: Predicted: 26 kDa; Apparent: 29 and 32 kDa.
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: cav-1
Immunogen: Recombinant His6-tagged CAV-1 fusion protein
Alternate Gene Names:
Alternate Antibody Name: 1F6
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q94051 
Immunogen Sequence: full length protein
Entrez Gene ID: 177815 
Additional Characterization:
Antibody Registry ID: AB_2244194 
Additional Information: CAV-1 is one of two caveolin homologs in C. elegans. Vertebrate caveolins are the major proteins required for the formation of plasma membrane invaginations called caveolae.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CAV1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CAV1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    PloS one
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    PloS one
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    PloS one
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    PloS one
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

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