CAV1

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SKU: CAV1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: CAV1
Available to For-Profits: Yes
Alternate Antibody Name: 1F6
Gene Name: cav-1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2244194 
Uniprot ID: Q94051 
RRID:  
Entrez Gene ID: 177815 
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged CAV-1 fusion protein
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: No
Antigen Name: Caveolin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/16/2010
Antigen Molecular Weight: Predicted: 26 kDa; Apparent: 29 and 32 kDa.
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Antigen Sequence:
Depositor Institution: Washington University Medical School
Antigen Species: C. elegans
Depositor Notes: Works well on C. elegans whole mounts. Stains caveolae.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: CAV-1 is one of two caveolin homologs in C. elegans. Vertebrate caveolins are the major proteins required for the formation of plasma membrane invaginations called caveolae.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CAV1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CAV1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

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