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DSHB Data Sheet

Catalog Fields

Antigen: RME-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts. Stains recycling endosomes.
Antigen Molecular Weight: Predicted: multiple isoforms from 63 to 94 kDa (65 kDa for isoform d); Apparent: 70 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: rme-1
Immunogen: Recombinant His6-tagged RME-1 (C-terminal 243 aa of isoform d) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5G11
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal 243 aa of isoform d
Uniprot ID: G5EFP1 
Immunogen Sequence: Partial protein
Entrez Gene ID: 179010 
Additional Characterization:  
Antibody Registry ID: AB_10571460 
Additional Information: RME-1 is an evolutionarily conserved protein specifically associated with recycling endosomes. The Uniprot ID provided is for isoform d (Receptor-mediated endocytosis 1 isoform IV).
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RME1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product RME1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    WB References
    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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