Catalog Fields
Antigen: RME-1
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans
Depositors Institution: Washington University Medical School
Antigen Molecular Weight: Predicted: multiple isoforms from 63 to 94 kDa (65 kDa for isoform d); Apparent: 70 kDa
Depositors Notes: Works on C. elegans whole mounts. Stains recycling endosomes.
Immunogen: Recombinant His6-tagged RME-1 (C-terminal 243 aa of isoform d) fusion protein
Gene: rme-1
Alternate Antibody Name: 5G11
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Uniprot ID: G5EFP1
Epitope Location or Sequence: C-terminal 243 aa of isoform d
Entrez Gene ID: 179010
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_10571460
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: RME-1 is an evolutionarily conserved protein specifically associated with recycling endosomes. The Uniprot ID provided is for isoform d (Receptor-mediated endocytosis 1 isoform IV).
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RME1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product RME1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
5 References
Initial Publication
A monoclonal antibody toolkit for C. elegans.
Nonet ML
PloS one 5.4 (2010 Apr 13): e10161.