DYN1

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$40.00
SKU: DYN1
View product citations for antibody DYN1 on CiteAb

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Available: 19

DSHB Data Sheet

Catalog Fields

Antigen: Dynamin
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts. Stains sites of clathrin-mediated endocytosis.
Antigen Molecular Weight: Predicted: 93 and 94 kDa; Apparent: 100 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: dyn-1
Immunogen: Recombinant His6-tagged DYN-1 fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5B1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P39055 
Immunogen Sequence: full length protein
Entrez Gene ID: 181644 
Additional Characterization:
Antibody Registry ID: AB_10572297 
Additional Information: DYN-1 is a homolog of dynamin 1 which regulates clathrin mediated endocytosis.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DYN1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product DYN1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    Germline stem cell arrest inhibits the collapse of somatic proteostasis early in Caenorhabditis elegans adulthood.
    Ben-Zvi A
    Aging cell 12.5 (2013 Oct): 814-22.

    GABAergic synaptic plasticity during a developmentally regulated sleep-like state in C. elegans.
    Raizen DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.44 (2011 Nov 2): 15932-43.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia.
    Soto MC
    Developmental biology 377.2 (2013 May 15): 319-32.

    Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets.
    Liu P
    Molecular and cellular proteomics : MCP 11.8 (2012 Aug): 317-28.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    Germline stem cell arrest inhibits the collapse of somatic proteostasis early in Caenorhabditis elegans adulthood.
    Ben-Zvi A
    Aging cell 12.5 (2013 Oct): 814-22.

    GABAergic synaptic plasticity during a developmentally regulated sleep-like state in C. elegans.
    Raizen DM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.44 (2011 Nov 2): 15932-43.

    Conservation of mRNA and protein expression during development of C. elegans.
    Rajewsky N
    Cell reports 6.3 (2014 Feb 13): 565-77.

    WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia.
    Soto MC
    Developmental biology 377.2 (2013 May 15): 319-32.

    Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets.
    Liu P
    Molecular and cellular proteomics : MCP 11.8 (2012 Aug): 317-28.

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