B3-1

(0) No Reviews yet
$40.00
SKU: B3-1-s
View product citations for antibody B3-1 on CiteAb

In Stock

Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: collagen type IX
Hybridoma Cells Available: No
Antigen Species: bovine
Depositor: Ye, X.-J. / Terato, K.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Tennessee, Memphis
Positive Tested Species Reactivity: Bovine, Human, Rat
Depositors Notes: Antigen chemical properties: protein. IHC note: recognizes type IX collagen in 95% ethanol-fixed paraffin embedded sections of human and rat cartilage.
Antigen Molecular Weight: 300 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B3-1 was deposited to the DSHB by Ye, X.-J. / Terato, K. (DSHB Hybridoma Product B3-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibodies against bovine type IX collagen (LMW fragment): production, characterization, and use for immunohistochemical localization studies.
    Yoo TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.3 (1991 Mar): 265-71.

    All References

    Monoclonal antibodies against bovine type IX collagen (LMW fragment): production, characterization, and use for immunohistochemical localization studies.
    Yoo TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.3 (1991 Mar): 265-71.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review