BIIG2
Catalog Fields
Antigen: integrin alpha-5
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Damsky, C.H.
Isotype: RIgG2b, kappa light chain
Depositors Institution: University of California
Host Species: rat
Depositors Notes: Stains aldehyde fixed cells and frozen sections of aldehyde fixed cells. Blocks cell attachment to Fibronectin only. Binds Protein G. Cell binding: stains cell surface of connective tissue cells, some embryonic epithelia. Doesn't appear to work for western blotting.
Positive Tested Species Reactivity: Bovine, Human, Rabbit
Gene: ITGA5
Antigen Molecular Weight: Predicted: 115 kDa; Apparent: 130 kDa non-reduced gel mobility, 120 kDa reduced gel mobility
Alternate Gene Names: FNRA, CD49e, VLA-5, VLA5A
Immunogen: EDTA-harvested human JAR choriocarcinoma cells
Clonality: Monoclonal
Alternate Antibody Name: anti-FnR
Epitope Mapped: No
Myeloma Strain: SP 2/0
Immunogen Sequence: Full length protein
Uniprot ID: P08648
Additional Information: Like most anti-integrin antibodies, AIIB2 does not blot well. Refer to the cited paper for protocol required for western blotting.
Entrez Gene ID: 3678
Antibody Registry ID: AB_528155
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BIIG2 was deposited to the DSHB by Damsky, C.H. (DSHB Hybridoma Product BIIG2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.
Damsky CH
The Journal of cell biology 110.6 (1990 Jun): 2175-84.