BIIG2
Catalog Fields
Antigen: integrin alpha-5
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Damsky, C.H.
Isotype: RIgG2b, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: University of California, San Franscisco
Positive Tested Species Reactivity: Bovine, Human, Rabbit
Depositors Notes: Stains aldehyde fixed cells and frozen sections of aldehyde fixed cells. Blocks cell attachment to Fibronectin only. Binds Protein G. Cell binding: stains cell surface of connective tissue cells, some embryonic epithelia. Doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 115 kDa; Apparent: 130 kDa non-reduced gel mobility, 120 kDa reduced gel mobility
Predicted Species Reactivity:
Gene: ITGA5
Immunogen: EDTA-harvested human JAR choriocarcinoma cells
Alternate Gene Names: FNRA, CD49e, VLA-5, VLA5A
Alternate Antibody Name: anti-FnR
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: P08648
Immunogen Sequence: Full length protein
Entrez Gene ID: 3678
Antibody Registry ID: AB_528155
Additional Information: Like most anti-integrin antibodies, AIIB2 does not blot well. Refer to the cited paper for protocol required for western blotting.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BIIG2 was deposited to the DSHB by Damsky, C.H. (DSHB Hybridoma Product BIIG2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.
Damsky CH
The Journal of cell biology 110.6 (1990 Jun): 2175-84.