BIIG2

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$40.00
SKU: BIIG2
View product citations for antibody BIIG2 on CiteAb

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Available: 174

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-5
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Damsky, C.H.
Isotype: RIgG2b, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: University of California
Positive Tested Species Reactivity: Bovine, Human, Rabbit
Depositors Notes: Stains aldehyde fixed cells and frozen sections of aldehyde fixed cells. Blocks cell attachment to Fibronectin only. Binds Protein G. Cell binding: stains cell surface of connective tissue cells, some embryonic epithelia. Doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 115 kDa; Apparent: 130 kDa non-reduced gel mobility, 120 kDa reduced gel mobility
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGA5
Immunogen: EDTA-harvested human JAR choriocarcinoma cells
Alternate Gene Names: FNRA, CD49e, VLA-5, VLA5A
Alternate Antibody Name: anti-FnR
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID: P08648 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3678 
Additional Characterization:
Antibody Registry ID: AB_528155 
Additional Information: Like most anti-integrin antibodies, AIIB2 does not blot well. Refer to the cited paper for protocol required for western blotting.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BIIG2 was deposited to the DSHB by Damsky, C.H. (DSHB Hybridoma Product BIIG2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References

    Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
    Damsky CH
    The Journal of cell biology 109.2 (1989 Aug): 877-89.

    The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.
    Damsky CH
    The Journal of cell biology 110.6 (1990 Jun): 2175-84.

    Different integrins mediate cell spreading, haptotaxis and lateral migration of HaCaT keratinocytes on fibronectin.
    Larjava H
    Cell adhesion and communication 7.3 (1999): 245-57.

    Integrins alpha5beta1, alphavbeta1, and alphavbeta6 collaborate in squamous carcinoma cell spreading and migration on fibronectin.
    Larjava H
    Experimental cell research 255.1 (2000 Feb 25): 10-7.

    Helicobacter pylori exploits cholesterol-rich microdomains for induction of NF-kappaB-dependent responses and peptidoglycan delivery in epithelial cells.
    Ferrero RL
    Infection and immunity 78.11 (2010 Nov): 4523-31.

    Simple coating with fibronectin fragment enhances stainless steel screw osseointegration in healthy and osteoporotic rats.
    García AJ
    Biomaterials 63. (2015 Sep): 137-45.

    Traction forces mediated by integrin signaling are necessary for definitive endoderm specification.
    Engler AJ
    Journal of cell science 128.10 (2015 May 15): 1961-8.

    A novel NOD1- and CagA-independent pathway of interleukin-8 induction mediated by the Helicobacter pylori type IV secretion system.
    Kwok T
    Cellular microbiology 15.4 (2013 Apr): 554-70.

    The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins.
    Williams JV
    Journal of virology 86.22 (2012 Nov): 12148-60.

    The role of integrin α2 in cell and matrix therapy that improves perfusion, viability and function of infarcted myocardium.
    Suuronen EJ
    Biomaterials 35.17 (2014 Jun): 4749-58.

    Recombinant laminin-8 (alpha(4)beta(1)gamma(1)). Production, purification,and interactions with integrins.
    Tryggvason K
    The Journal of biological chemistry 275.20 (2000 May 19): 14853-9.

    FACS References
    All References

    Distribution patterns of extracellular matrix components and adhesion receptors are intricately modulated during first trimester cytotrophoblast differentiation along the invasive pathway, in vivo.
    Fisher SJ
    The Journal of clinical investigation 89.1 (1992 Jan): 210-22.

    Integrin switching regulates normal trophoblast invasion.
    Fisher SJ
    Development (Cambridge, England) 120.12 (1994 Dec): 3657-66.

    Helicobacter pylori exploits cholesterol-rich microdomains for induction of NF-kappaB-dependent responses and peptidoglycan delivery in epithelial cells.
    Ferrero RL
    Infection and immunity 78.11 (2010 Nov): 4523-31.

    The role of integrin α2 in cell and matrix therapy that improves perfusion, viability and function of infarcted myocardium.
    Suuronen EJ
    Biomaterials 35.17 (2014 Jun): 4749-58.

    Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
    Damsky CH
    The Journal of cell biology 109.2 (1989 Aug): 877-89.

    Cooperative signaling by alpha 5 beta 1 and alpha 4 beta 1 integrins regulates metalloproteinase gene expression in fibroblasts adhering to fibronectin.
    Damsky CH
    The Journal of cell biology 129.3 (1995 May): 867-79.

    Functional roles of the bisecting GlcNAc in integrin-mediated cell adhesion.
    Gu J
    Methods in enzymology 480. (2010): 445-59.

    The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin.
    Damsky CH
    The Journal of cell biology 110.6 (1990 Jun): 2175-84.

    Different integrins mediate cell spreading, haptotaxis and lateral migration of HaCaT keratinocytes on fibronectin.
    Larjava H
    Cell adhesion and communication 7.3 (1999): 245-57.

    Integrins alpha5beta1, alphavbeta1, and alphavbeta6 collaborate in squamous carcinoma cell spreading and migration on fibronectin.
    Larjava H
    Experimental cell research 255.1 (2000 Feb 25): 10-7.

    Simple coating with fibronectin fragment enhances stainless steel screw osseointegration in healthy and osteoporotic rats.
    García AJ
    Biomaterials 63. (2015 Sep): 137-45.

    Traction forces mediated by integrin signaling are necessary for definitive endoderm specification.
    Engler AJ
    Journal of cell science 128.10 (2015 May 15): 1961-8.

    A novel NOD1- and CagA-independent pathway of interleukin-8 induction mediated by the Helicobacter pylori type IV secretion system.
    Kwok T
    Cellular microbiology 15.4 (2013 Apr): 554-70.

    The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins.
    Williams JV
    Journal of virology 86.22 (2012 Nov): 12148-60.

    Recombinant laminin-8 (alpha(4)beta(1)gamma(1)). Production, purification,and interactions with integrins.
    Tryggvason K
    The Journal of biological chemistry 275.20 (2000 May 19): 14853-9.

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