P5D2

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$40.00
SKU: P5D2
View product citations for antibody P5D2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 (CD29), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1
Depositors Institution: Fred Hutchison Cancer Research Center
Host Species: mouse
Depositors Notes: Inhibits binding of beta1 integrins. This antibody doesn't appear to work for western blotting.
Positive Tested Species Reactivity: Human
Gene: ITGB1
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Alternate Gene Names: CD29, GPIIA, FNRB, MDF2, MSK12, VLA-4beta
Immunogen: Human epidermal keratinocytes
Clonality: Monoclonal
Alternate Antibody Name: CD29
Epitope Mapped: Yes
Myeloma Strain: Fox NY
Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218)
Uniprot ID: P05556 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3688 
Antibody Registry ID: AB_528308 
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5D2 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References
    FB References

    Chlamydia trachomatis does not bind to alpha beta 1 integrins to colonize a human endometrial epithelial cell line cultured in vitro.
    Wayner EA
    Microbial pathogenesis 17.3 (1994 Sep): 159-66.

    Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1).
    LeBien TW
    Blood 81.9 (1993 May 1): 2272-82.

    Activation of 72-kDa type IV collagenase/gelatinase by normal fibroblasts in collagen lattices is mediated by integrin receptors but is not related to lattice contraction.
    Eisen AZ
    Experimental cell research 213.2 (1994 Aug): 365-74.

    Interactions of type IV collagen and its domains with human mesangial cells.
    Tsilibary EC
    The Journal of biological chemistry 273.20 (1998 May 15): 12244-9.

    Interferon-alpha restores normal adhesion of chronic myelogenous leukemia hematopoietic progenitors to bone marrow stroma by correcting impaired beta 1 integrin receptor function.
    Verfaillie CM
    The Journal of clinical investigation 94.1 (1994 Jul): 384-91.

    The decreased adhesion of Y79 retinoblastoma cells to extracellular matrix proteins is due to a deficit of integrin receptors.
    Cameron JD
    Investigative ophthalmology & visual science 35.6 (1994 May): 2820-33.

    CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells.
    Hauck CR
    The Journal of cell biology 170.5 (2005 Aug 29): 825-36.

    Delineating breast cancer cell interactions with engineered bone microenvironments.
    Hutmacher DW
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 28.6 (2013 Jun): 1399-411.

    An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation.
    Gu J
    The Journal of biological chemistry 289.30 (2014 Jul 25): 20694-705.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    FACS References
    Epitope Map References
    All References

    Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.
    Wilke MS
    The American journal of pathology 148.5 (1996 May): 1445-61.

    Localization of candidate stem and progenitor cell markers within the human cornea, limbus, and bulbar conjunctiva in vivo and in cell culture.
    Griffith M
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 288.8 (2006 Aug): 921-31.

    A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin.
    Ivaska J
    PloS one 8.8 (2013): e70696.

    Integrin alpha 4 beta 1 and glycoprotein IV (CD36) are expressed on circulating reticulocytes in sickle cell anemia.
    Parise LV
    Blood 82.12 (1993 Dec 15): 3548-55.

    Interactions of type IV collagen and its domains with human mesangial cells.
    Tsilibary EC
    The Journal of biological chemistry 273.20 (1998 May 15): 12244-9.

    Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65.
    Wang Y
    Nature communications 4. (2013): 1659.

    Transforming growth factor beta receptor 2 (TGFBR2) changes sialylation in the microsatellite unstable (MSI) Colorectal cancer cell line HCT116.
    Gebert J
    PloS one 8.2 (2013): e57074.

    Chlamydia trachomatis does not bind to alpha beta 1 integrins to colonize a human endometrial epithelial cell line cultured in vitro.
    Wayner EA
    Microbial pathogenesis 17.3 (1994 Sep): 159-66.

    Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1).
    LeBien TW
    Blood 81.9 (1993 May 1): 2272-82.

    Activation of 72-kDa type IV collagenase/gelatinase by normal fibroblasts in collagen lattices is mediated by integrin receptors but is not related to lattice contraction.
    Eisen AZ
    Experimental cell research 213.2 (1994 Aug): 365-74.

    Interferon-alpha restores normal adhesion of chronic myelogenous leukemia hematopoietic progenitors to bone marrow stroma by correcting impaired beta 1 integrin receptor function.
    Verfaillie CM
    The Journal of clinical investigation 94.1 (1994 Jul): 384-91.

    The decreased adhesion of Y79 retinoblastoma cells to extracellular matrix proteins is due to a deficit of integrin receptors.
    Cameron JD
    Investigative ophthalmology & visual science 35.6 (1994 May): 2820-33.

    CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells.
    Hauck CR
    The Journal of cell biology 170.5 (2005 Aug 29): 825-36.

    Delineating breast cancer cell interactions with engineered bone microenvironments.
    Hutmacher DW
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 28.6 (2013 Jun): 1399-411.

    An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation.
    Gu J
    The Journal of biological chemistry 289.30 (2014 Jul 25): 20694-705.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    A novel monoclonal antibody recognizing a cation-dependent epitope within the regulatory loop of human beta(1) integrin (CD29).
    Simmons PJ
    Hybridoma and hybridomics 21.4 (2002 Aug): 253-60.

    Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin.
    Clegg DO
    Stem cells and development 19.8 (2010 Aug): 1231-40.

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