CePAS7

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SKU: CePAS7

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: CePAS7
Available to For-Profits: Yes
Alternate Antibody Name: PAS7, 1A1
Gene Symbol: pas-7
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_10571458 
Uniprot ID: Q09583 
RRID:  
Entrez Gene ID: 174571 
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged PAS-7 fusion protein
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: No
Antigen Name: PAS-7
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/16/2010
Antigen Molecular Weight: Predicted: 28 kDa; Apparent: 28 kDa
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Antigen Sequence:
Depositor Institution: Washington University Medical School
Antigen Species: C. elegans
Depositor Notes: Works on C. elegans whole mounts.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: The Proteasome subunit alpha type-3, PAS-7, is a ortholog of an evolutionarily conserved subunit of the 26S proteasome subunit which is involved in regulated protein degradation.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CePAS7 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CePAS7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
    Deuerling E
    Science (New York, N.Y.) 348.6231 (2015 Apr 10): 201-7.

    Aging and SKN-1-dependent Loss of 20S Proteasome Adaptation to Oxidative Stress in C. elegans.
    Davies KJ
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.2 (2017 Feb): 143-151.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
    Deuerling E
    Science (New York, N.Y.) 348.6231 (2015 Apr 10): 201-7.

    Aging and SKN-1-dependent Loss of 20S Proteasome Adaptation to Oxidative Stress in C. elegans.
    Davies KJ
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.2 (2017 Feb): 143-151.

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