CePAS7

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$40.00
SKU: CePAS7
View product citations for antibody CePAS7 on CiteAb

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Available: 78

DSHB Data Sheet

Catalog Fields

Antigen: PAS-7
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works on C. elegans whole mounts.
Antigen Molecular Weight: Predicted: 28 kDa; Apparent: 28 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: pas-7
Immunogen: Recombinant His6-tagged PAS-7 fusion protein
Alternate Gene Names:
Alternate Antibody Name: PAS7, 1A1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q09583 
Immunogen Sequence: full length protein
Entrez Gene ID: 174571 
Additional Characterization:
Antibody Registry ID: AB_10571458 
Additional Information: The Proteasome subunit alpha type-3, PAS-7, is a ortholog of an evolutionarily conserved subunit of the 26S proteasome subunit which is involved in regulated protein degradation.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CePAS7 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CePAS7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
    Deuerling E
    Science (New York, N.Y.) 348.6231 (2015 Apr 10): 201-7.

    Aging and SKN-1-dependent Loss of 20S Proteasome Adaptation to Oxidative Stress in C. elegans.
    Davies KJ
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.2 (2017 Feb): 143-151.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.
    Podbilewicz B
    Cell reports 14.6 (2016 Feb 16): 1517-1527.

    The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum.
    Deuerling E
    Science (New York, N.Y.) 348.6231 (2015 Apr 10): 201-7.

    Aging and SKN-1-dependent Loss of 20S Proteasome Adaptation to Oxidative Stress in C. elegans.
    Davies KJ
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.2 (2017 Feb): 143-151.

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