D18
Catalog Fields
Clone ID/Product Name: D18
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Lamc1
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_2281095
Uniprot ID: F1MAA7
Entrez Gene ID: 117036
Clonality: Monoclonal
Immunogen: Adult rat glomerular basement membrane.
Clone:
Immunogen Sequence: full length protein
Myeloma Strain: fox NY
Epitope Mapped: No
Antigen Name: laminin, gamma-1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/22/1992
Antigen Molecular Weight: Predicted: 174 kDa; Apparent: 200 kDa
Depositor: Sanes, J.R.
Antigen Sequence:
Depositor Institution: Harvard University
Antigen Species: Rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Guinea Pig, Human, Rat
Additional Information: The former name of laminin gamma-1 was laminin B2.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D18 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product D18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
Hunter DD
The Journal of cell biology 111.4 (1990 Oct): 1685-99.