D18

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$40.00
SKU: D18
View product citations for antibody D18 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: laminin, gamma-1
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Sanes, J.R.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard University
Positive Tested Species Reactivity: Guinea Pig, Human, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 174 kDa; Apparent: 200 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Lamc1
Immunogen: Adult rat glomerular basement membrane.
Alternate Gene Names: B2e
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: fox NY
Epitope Location or Sequence:
Uniprot ID: F1MAA7 
Immunogen Sequence: full length protein
Entrez Gene ID: 117036 
Additional Characterization:  
Antibody Registry ID: AB_2281095 
Additional Information: The former name of laminin gamma-1 was laminin B2.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D18 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product D18)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Survival of Schwann cells in chronically denervated skeletal muscles.
    Carlson BM
    Acta neuropathologica 103.6 (2002 Jun): 565-74.

    Aging of skeletal muscle does not affect the response of satellite cells to denervation.
    Carlson BM
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.7 (2003 Jul): 853-63.

    Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane.
    Shimizu H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.1 (2006 Jan): 109-18.

    Mesenchymal Stromal Cells for Sphincter Regeneration: Role of Laminin Isoforms upon Myogenic Differentiation.
    Klein G
    PloS one 10.9 (2015): e0137419.

    BeWo choriocarcinoma cells produce laminin 10.
    Aplin JD
    The Biochemical journal 332 ( Pt 2). (1998 Jun 1): 491-8.

    Collagen IV alpha 3, alpha 4, and alpha 5 chains in rodent basal laminae: sequence, distribution, association with laminins, and developmental switches.
    Sanes JR
    The Journal of cell biology 127.3 (1994 Nov): 879-91.

    Developmentally regulated interactions of human thymocytes with different laminin isoforms.
    Klein G
    Immunology 105.4 (2002 Apr): 407-18.

    Deposition of the NG2 proteoglycan at nodes of Ranvier in the peripheral nervous system.
    Levine JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.20 (2001 Oct 15): 8119-28.

    Functional integrin subunits regulating cell-matrix interactions in the intervertebral disc.
    Setton LA
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 25.6 (2007 Jun): 829-40.

    Differential laminin isoform expression in the developing rat olfactory system.
    Schwarting GA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 187-96.

    WB References
    IHC References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    IP References

    Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Sanes JR
    The Journal of biological chemistry 267.3 (1992 Jan 25): 2014-22.

    All References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Survival of Schwann cells in chronically denervated skeletal muscles.
    Carlson BM
    Acta neuropathologica 103.6 (2002 Jun): 565-74.

    Aging of skeletal muscle does not affect the response of satellite cells to denervation.
    Carlson BM
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.7 (2003 Jul): 853-63.

    Colocalization of multiple laminin isoforms predominantly beneath hemidesmosomes in the upper lamina densa of the epidermal basement membrane.
    Shimizu H
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.1 (2006 Jan): 109-18.

    Mesenchymal Stromal Cells for Sphincter Regeneration: Role of Laminin Isoforms upon Myogenic Differentiation.
    Klein G
    PloS one 10.9 (2015): e0137419.

    BeWo choriocarcinoma cells produce laminin 10.
    Aplin JD
    The Biochemical journal 332 ( Pt 2). (1998 Jun 1): 491-8.

    Collagen IV alpha 3, alpha 4, and alpha 5 chains in rodent basal laminae: sequence, distribution, association with laminins, and developmental switches.
    Sanes JR
    The Journal of cell biology 127.3 (1994 Nov): 879-91.

    Developmentally regulated interactions of human thymocytes with different laminin isoforms.
    Klein G
    Immunology 105.4 (2002 Apr): 407-18.

    Deposition of the NG2 proteoglycan at nodes of Ranvier in the peripheral nervous system.
    Levine JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.20 (2001 Oct 15): 8119-28.

    Functional integrin subunits regulating cell-matrix interactions in the intervertebral disc.
    Setton LA
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 25.6 (2007 Jun): 829-40.

    Differential laminin isoform expression in the developing rat olfactory system.
    Schwarting GA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 187-96.

    Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
    Sanes JR
    The Journal of biological chemistry 267.3 (1992 Jan 25): 2014-22.

    Reparative myogenesis in long-term denervated skeletal muscles of adult rats results in a reduction of the satellite cell population.
    Carlson BM
    The Anatomical record 263.2 (2001 Jun 1): 139-54.

    MyoD and myogenin protein expression in skeletal muscles of senile rats.
    Carlson BM
    Cell and tissue research 311.3 (2003 Mar): 401-16.

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