2E8
Catalog Fields
Antigen: laminin, gamma-1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Engvall, E.S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: La Jolla Cancer Research Foundation
Positive Tested Species Reactivity: Canine, Guinea Pig, Human, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 174 kDa; Apparent: 200 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: LAMC1
Immunogen: human laminin from term placenta
Alternate Gene Names: MGC87297 LAMB2
Alternate Antibody Name: Anti-Laminin ?1,anti Laminin B2 Chain, LM?1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Close to the junction between domains II and III
Uniprot ID: P11047
Immunogen Sequence: Full length protein
Entrez Gene ID: 3915
Additional Characterization:
Antibody Registry ID: AB_2134060
Additional Information: Better western blot results using non-reducing conditions. 2E8 doesn't cross-react with mouse antigen. 2E8 doesn't appear to affect laminin gamma-1 function.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2E8 was deposited to the DSHB by Engvall, E.S. (DSHB Hybridoma Product 2E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
24 References
Initial Publication
Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
Manthorpe M
The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2457-65.