2E8
Catalog Fields
Clone ID/Product Name: 2E8
Available to For-Profits: Yes
Alternate Antibody Name: Anti-Laminin gamma1,anti Laminin B2 Chain, LM gamma1
Gene Symbol: LAMC1
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Entrez Gene ID: 3915
Clonality: Monoclonal
Immunogen: human laminin from term placenta
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63.Ag8.653
Epitope Mapped: Yes
Antigen Name: laminin, gamma-1
Epitope Location or Sequence: Close to the junction between domains II and III
Alternate Antigen Name:
Deposit Date: 4/8/1987
Antigen Molecular Weight: Predicted: 174 kDa; Apparent: 200 kDa
Depositor: Engvall, E.S.
Antigen Sequence:
Depositor Institution: La Jolla Cancer Research Foundation
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Canine, Guinea Pig, Human, Rat
Additional Information: Better western blot results using non-reducing conditions. 2E8 doesn't cross-react with mouse antigen. 2E8 doesn't appear to affect laminin gamma-1 function.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2E8 was deposited to the DSHB by Engvall, E.S. (DSHB Hybridoma Product 2E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
28 References
Initial Publication
Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
Manthorpe M
The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2457-65.