2E8
Catalog Fields
Antigen: laminin, gamma-1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Engvall, E.S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: La Jolla Cancer Research Foundation
Positive Tested Species Reactivity: Canine, Guinea Pig, Human, Rat
Depositors Notes:
Predicted Species Reactivity:
Gene: LAMC1
Immunogen: human laminin from term placenta
Alternate Gene Names: MGC87297 LAMB2
Alternate Antibody Name: Anti-Laminin ?1,anti Laminin B2 Chain, LM?1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Close to the junction between domains II and III
Uniprot ID: P11047
Immunogen Sequence: Full length protein
Entrez Gene ID: 3915
Antibody Registry ID: AB_2134060
Additional Information: Better western blot results using non-reducing conditions. 2E8 doesn't cross-react with mouse antigen. 2E8 doesn't appear to affect laminin gamma-1 function.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2E8 was deposited to the DSHB by Engvall, E.S. (DSHB Hybridoma Product 2E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
26 References
Initial Publication
Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
Manthorpe M
The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2457-65.