2E8

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$40.00
SKU: 2E8
View product citations for antibody 2E8 on CiteAb

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Available: 50

DSHB Data Sheet

Catalog Fields

Antigen: laminin, gamma-1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Engvall, E.S.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: La Jolla Cancer Research Foundation
Positive Tested Species Reactivity: Canine, Guinea Pig, Human, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 174 kDa; Apparent: 200 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LAMC1
Immunogen: human laminin from term placenta
Alternate Gene Names: MGC87297 LAMB2
Alternate Antibody Name: Anti-Laminin ?1,anti Laminin B2 Chain, LM?1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63.Ag8.653
Epitope Location or Sequence: Close to the junction between domains II and III
Uniprot ID: P11047 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3915 
Additional Characterization:
Antibody Registry ID: AB_2134060 
Additional Information: Better western blot results using non-reducing conditions. 2E8 doesn't cross-react with mouse antigen. 2E8 doesn't appear to affect laminin gamma-1 function.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2E8 was deposited to the DSHB by Engvall, E.S. (DSHB Hybridoma Product 2E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
    Manthorpe M
    The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2457-65.

    Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms.
    Kenney MC
    Laboratory investigation; a journal of technical methods and pathology 72.4 (1995 Apr): 461-73.

    Immunophenotyping of congenital myopathies: disorganization of sarcomeric, cytoskeletal and extracellular matrix proteins.
    Ramaekers FC
    Journal of the neurological sciences 129.2 (1995 Apr): 199-213.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
    Sanes JR
    Cell regulation 1.10 (1990 Sep): 731-40.

    BeWo choriocarcinoma cells produce laminin 10.
    Aplin JD
    The Biochemical journal 332 ( Pt 2). (1998 Jun 1): 491-8.

    Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.
    Wagner KR
    Skeletal muscle 6. (2016): 14.

    Novel Function of Serine Protease HTRA1 in Inhibiting Adipogenic Differentiation of Human Mesenchymal Stem Cells via MAP Kinase-Mediated MMP Upregulation.
    Richards PJ
    Stem cells (Dayton, Ohio) 34.6 (2016 Jun): 1601-14.

    Identification and localization of lamina cribrosa cells in the human optic nerve head.
    Clark AF
    Experimental eye research 147. (2016 Jun): 94-97.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    WB References

    The presynaptic calcium channel is part of a transmembrane complex linking a synaptic laminin (alpha4beta2gamma1) with non-erythroid spectrin.
    Carlson SS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.3 (2000 Feb 1): 1009-19.

    Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
    Manthorpe M
    The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2457-65.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Subunit structure of a laminin-binding integrin and localization of its binding site on laminin.
    Ruoslahti E
    The Journal of biological chemistry 264.32 (1989 Nov 15): 19034-8.

    Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
    Sanes JR
    Cell regulation 1.10 (1990 Sep): 731-40.

    Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions.
    Marinkovich MP
    The Journal of investigative dermatology 126.2 (2006 Feb): 432-40.

    Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains.
    Patarroyo M
    Matrix biology : journal of the International Society for Matrix Biology 19.2 (2000 May): 163-7.

    IHC References

    Muscle contraction and force: the importance of an ancillary network, nutrient supply and waste removal.
    Harrison AP
    International journal of molecular sciences 9.8 (2008 Aug): 1472-88.

    Biochemical and mechanical environment cooperatively regulate skeletal muscle regeneration.
    Simon HG
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.6 (2012 Jun): 2538-45.

    Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream.
    Horwitz AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.9 (2002 May 1): 3568-79.

    Resistance exercise reduces skeletal muscle cachexia and improves muscle function in rheumatoid arthritis.
    Alway SE
    Case reports in medicine 2011. (2011): 205691.

    The presynaptic calcium channel is part of a transmembrane complex linking a synaptic laminin (alpha4beta2gamma1) with non-erythroid spectrin.
    Carlson SS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.3 (2000 Feb 1): 1009-19.

    The neurite-promoting domain of human laminin promotes attachment and induces characteristic morphology in non-neuronal cells.
    Engvall E
    Experimental cell research 177.1 (1988 Jul): 186-98.

    Distribution of extracellular matrix components during early embryonic development in the macaque.
    Hendrickx AG
    Acta anatomica 146.1 (1993): 3-13.

    Characterization of the extracellular matrix during somitogenesis in the long-tailed monkey (Macaca fascicularis).
    Hendrickx AG
    Acta anatomica 146.4 (1993): 223-33.

    Cellular and morphological changes with EAA supplementation before and after total knee arthroplasty.
    Dreyer HC
    Journal of applied physiology (Bethesda, Md. : 1985) 127.2 (2019 Aug 1): 531-545.

    IP References
    ELISA References
    Epitope Map References
    All References

    Muscle contraction and force: the importance of an ancillary network, nutrient supply and waste removal.
    Harrison AP
    International journal of molecular sciences 9.8 (2008 Aug): 1472-88.

    Biochemical and mechanical environment cooperatively regulate skeletal muscle regeneration.
    Simon HG
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.6 (2012 Jun): 2538-45.

    Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream.
    Horwitz AF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 22.9 (2002 May 1): 3568-79.

    Resistance exercise reduces skeletal muscle cachexia and improves muscle function in rheumatoid arthritis.
    Alway SE
    Case reports in medicine 2011. (2011): 205691.

    The presynaptic calcium channel is part of a transmembrane complex linking a synaptic laminin (alpha4beta2gamma1) with non-erythroid spectrin.
    Carlson SS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 20.3 (2000 Feb 1): 1009-19.

    The neurite-promoting domain of human laminin promotes attachment and induces characteristic morphology in non-neuronal cells.
    Engvall E
    Experimental cell research 177.1 (1988 Jul): 186-98.

    Distribution of extracellular matrix components during early embryonic development in the macaque.
    Hendrickx AG
    Acta anatomica 146.1 (1993): 3-13.

    Characterization of the extracellular matrix during somitogenesis in the long-tailed monkey (Macaca fascicularis).
    Hendrickx AG
    Acta anatomica 146.4 (1993): 223-33.

    Cellular and morphological changes with EAA supplementation before and after total knee arthroplasty.
    Dreyer HC
    Journal of applied physiology (Bethesda, Md. : 1985) 127.2 (2019 Aug 1): 531-545.

    Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site.
    Manthorpe M
    The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2457-65.

    Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms.
    Kenney MC
    Laboratory investigation; a journal of technical methods and pathology 72.4 (1995 Apr): 461-73.

    Immunophenotyping of congenital myopathies: disorganization of sarcomeric, cytoskeletal and extracellular matrix proteins.
    Ramaekers FC
    Journal of the neurological sciences 129.2 (1995 Apr): 199-213.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere.
    Hunter DD
    The Journal of cell biology 111.4 (1990 Oct): 1685-99.

    Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits.
    Sanes JR
    Cell regulation 1.10 (1990 Sep): 731-40.

    BeWo choriocarcinoma cells produce laminin 10.
    Aplin JD
    The Biochemical journal 332 ( Pt 2). (1998 Jun 1): 491-8.

    Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.
    Wagner KR
    Skeletal muscle 6. (2016): 14.

    Novel Function of Serine Protease HTRA1 in Inhibiting Adipogenic Differentiation of Human Mesenchymal Stem Cells via MAP Kinase-Mediated MMP Upregulation.
    Richards PJ
    Stem cells (Dayton, Ohio) 34.6 (2016 Jun): 1601-14.

    Identification and localization of lamina cribrosa cells in the human optic nerve head.
    Clark AF
    Experimental eye research 147. (2016 Jun): 94-97.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    Subunit structure of a laminin-binding integrin and localization of its binding site on laminin.
    Ruoslahti E
    The Journal of biological chemistry 264.32 (1989 Nov 15): 19034-8.

    Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions.
    Marinkovich MP
    The Journal of investigative dermatology 126.2 (2006 Feb): 432-40.

    Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains.
    Patarroyo M
    Matrix biology : journal of the International Society for Matrix Biology 19.2 (2000 May): 163-7.

    Monoclonal antibodies to human laminin α4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of α6β1 integrin and MCAM to α4-laminins.
    Patarroyo M
    Matrix biology : journal of the International Society for Matrix Biology 36. (2014 Jun): 5-14.

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