D71E2
Catalog Fields
Clone ID/Product Name: D71E2
Available to For-Profits: Yes
Alternate Antibody Name: alpha5D7
Gene Symbol: Itga5
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 378932
Clonality: Monoclonal
Immunogen: 11 day chicken embryo denatured proteins electroeluted from the region of the chick embryo integrins.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: integrin alpha-5, avian
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/20/1993
Antigen Molecular Weight: Predicted: 113kDa; Apparent: 150 kDa
Depositor: Horwitz, A.F.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Chicken
Depositor Notes: This antibody has been generated against denatured protein; it immunoblots well, immunostains fixed tissue sections best when the sections are first heat denatured (methanol fixation works well for tissue culture cells), and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but, doesn't appear to precipitate the native receptor.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Amphibian, Avian
Additional Information: This antibody gives the same staining than A21F7 (alpha5A2; an anti-integrin alpha 5 clone also in the DSHB collection). These two mAbs are sometimes used as a mix to obtain stronger signals.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot