D71E2

(0) No Reviews yet
$40.00
SKU: D71E2
View product citations for antibody D71E2 on CiteAb

In Stock

Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-5, avian
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Horwitz, A.F.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Amphibian, Avian
Depositors Notes: This antibody has been generated against denatured protein; it immunoblots well, immunostains fixed tissue sections best when the sections are first heat denatured (methanol fixation works well for tissue culture cells), and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but, doesn't appear to precipitate the native receptor.
Antigen Molecular Weight: Predicted: 113kDa; Apparent: 150 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Itga5
Immunogen: 11 day chicken embryo denatured proteins electroeluted from the region of the chick embryo integrins.
Alternate Gene Names:
Alternate Antibody Name: alpha5D7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: X2C5I8 
Immunogen Sequence: Full length protein
Entrez Gene ID: 378932 
Additional Characterization:
Antibody Registry ID: AB_528154 
Additional Information: This antibody gives the same staining than A21F7 (alpha5A2; an anti-integrin alpha 5 clone also in the DSHB collection). These two mAbs are sometimes used as a mix to obtain stronger signals.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D71E2 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product D71E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development.
    Horwitz AF
    Development (Cambridge, England) 113.1 (1991 Sep): 327-37.

    IF References

    Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development.
    Horwitz AF
    Development (Cambridge, England) 113.1 (1991 Sep): 327-37.

    The alpha 5 beta 1 integrin associates with a dystrophin-containing lattice during muscle development.
    Horwitz AF
    Developmental biology 152.2 (1992 Aug): 209-20.

    Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.
    Kim J
    Cell motility and the cytoskeleton 45.1 (2000 Jan): 27-41.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Characterization of chicken epidermal dendritic cells.
    Magyar A
    Immunology 119.2 (2006 Oct): 278-88.

    WB References
    IP References

    Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development.
    Horwitz AF
    Development (Cambridge, England) 113.1 (1991 Sep): 327-37.

    All References

    Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development.
    Horwitz AF
    Development (Cambridge, England) 113.1 (1991 Sep): 327-37.

    The alpha 5 beta 1 integrin associates with a dystrophin-containing lattice during muscle development.
    Horwitz AF
    Developmental biology 152.2 (1992 Aug): 209-20.

    Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.
    Kim J
    Cell motility and the cytoskeleton 45.1 (2000 Jan): 27-41.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Characterization of chicken epidermal dendritic cells.
    Magyar A
    Immunology 119.2 (2006 Oct): 278-88.

    Transformation of chicken embryo fibroblasts by v-src uncouples beta1 integrin-mediated outside-in but not inside-out signaling.
    Boettiger DE
    Molecular and cellular biology 21.21 (2001 Nov): 7295-306.

    The integrin-binding motif RGDS induces protein tyrosine phosphorylation without activation in Bufo arenarum (Amphibia) oocytes.
    Coux G
    Reproduction (Cambridge, England) 141.5 (2011 May): 581-93.

    Altered expression of integrins in RSV-transformed chick epiphyseal chondrocytes.
    Gionti E
    Biochimie 85.5 (2003 May): 483-92.

    Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility.
    Condic ML
    The Journal of cell biology 167.3 (2004 Nov 8): 545-54.

    Neural crest motility and integrin regulation are distinct in cranial and trunk populations.
    Condic ML
    Developmental biology 259.2 (2003 Jul 15): 288-302.

    Sensory neuron subtypes have unique substratum preference and receptor expression before target innervation.
    Condic ML
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.5 (2003 Mar 1): 1781-91.

    Fibronectin assembly during early embryo development: A versatile communication system between cells and tissues.
    Thorsteinsdóttir S
    Developmental dynamics : an official publication of the American Association of Anatomists 245.4 (2016 Apr): 520-35.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review