D71E2
Catalog Fields
Antigen: integrin alpha-5, avian
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Horwitz, A.F.
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Virginia
Host Species: mouse
Depositors Notes: This antibody has been generated against denatured protein; it immunoblots well, immunostains fixed tissue sections best when the sections are first heat denatured (methanol fixation works well for tissue culture cells), and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but, doesn't appear to precipitate the native receptor.
Positive Tested Species Reactivity: Amphibian, Avian
Gene: Itga5
Antigen Molecular Weight: Predicted: 113kDa; Apparent: 150 kDa
Clonality: Monoclonal
Immunogen: 11 day chicken embryo denatured proteins electroeluted from the region of the chick embryo integrins.
Epitope Mapped: No
Alternate Antibody Name: alpha5D7
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Additional Information: This antibody gives the same staining than A21F7 (alpha5A2; an anti-integrin alpha 5 clone also in the DSHB collection). These two mAbs are sometimes used as a mix to obtain stronger signals.
Uniprot ID: X2C5I8
Entrez Gene ID: 378932
Antibody Registry ID: AB_528154
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D71E2 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product D71E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development.
Horwitz AF
Development (Cambridge, England) 113.1 (1991 Sep): 327-37.