D71E2
Catalog Fields
Antigen: integrin alpha-5, avian
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Horwitz, A.F.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Amphibian, Avian
Depositors Notes: This antibody has been generated against denatured protein; it immunoblots well, immunostains fixed tissue sections best when the sections are first heat denatured (methanol fixation works well for tissue culture cells), and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but, doesn't appear to precipitate the native receptor.
Predicted Species Reactivity:
Gene: Itga5
Immunogen: 11 day chicken embryo denatured proteins electroeluted from the region of the chick embryo integrins.
Alternate Gene Names:
Alternate Antibody Name: alpha5D7
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: X2C5I8
Immunogen Sequence: Full length protein
Entrez Gene ID: 378932
Antibody Registry ID: AB_528154
Additional Information: This antibody gives the same staining than A21F7 (alpha5A2; an anti-integrin alpha 5 clone also in the DSHB collection). These two mAbs are sometimes used as a mix to obtain stronger signals.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D71E2 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product D71E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Down-regulation of the chicken alpha 5 beta 1 integrin fibronectin receptor during development.
Horwitz AF
Development (Cambridge, England) 113.1 (1991 Sep): 327-37.