Antibody Name: D71E2
Antigen:integrin alpha-5, avian
Catalog Fields
Identity
Clone ID / Antibody Name: D71E2
Alternate Antibody Name: alpha5D7
Antigen Name: integrin alpha-5, avian
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: Itga5
Entrez Gene ID: 378932
Antigen Sequence: —
Antigen Species: Chicken
Antigen Mol. Weight: Predicted: 113kDa; Apparent: 150 kDa
Uniprot ID: X2C5I8
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1, kappa light chain
Myeloma Strain: SP2/0
Antibody Registry / RRID: AB_528154
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: 11 day chicken embryo denatured proteins electroeluted from the region of the chick embryo integrins.
Immunogen Sequence: Full length protein
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: This antibody gives the same staining than A21F7 (alpha5A2; an anti-integrin alpha 5 clone also in the DSHB collection). These two mAbs are sometimes used as a mix to obtain stronger signals.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Amphibian, Avian
Predicted Reactivity: —
Depositor
Depositor Name: A.F. Horwitz
Institution: University of Virginia
Deposit Date: April 20, 1993
Depositor Notes: This antibody has been generated against denatured protein; it immunoblots well, immunostains fixed tissue sections best when the sections are first heat denatured (methanol fixation works well for tissue culture cells), and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but, doesn't appear to precipitate the native receptor.
Recommended Applications
IF
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
▼
Could not load UniProt data for X2C5I8