CSAT

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$40.00
SKU: CSAT
View product citations for antibody CSAT on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Integrin beta-1 (avian)
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: Horwitz, A.F.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Chick, Quail
Depositors Notes: The cell substrate attachment antibody (CSAT) blocks migrationof neural crest in vivo, prevents neurite outgrowth and blocks myoblast fusion and differentiation in vitro. The antigenic site recognized by CSAT is sensitive to denaturation.
Antigen Molecular Weight: 120 kDa and 160 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGB1
Immunogen: Membrane blebs from 52-hr cultured myoblasts
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP 2/0-Ag14
Epitope Location or Sequence: N-terminus a.a. 1-160
Uniprot ID: P07228 
Immunogen Sequence: Full length protein
Entrez Gene ID: 374058 
Additional Characterization:
Antibody Registry ID: AB_528142 
Additional Information: In vivo injection of CSAT may be localized in frozen sections (PMID: 2944780). RRID:AB_528142
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CSAT was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product CSAT)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

27 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
    Horwitz AF
    The Journal of cell biology 95.2 Pt 1 (1982 Nov): 654-66.

    IF References

    A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
    Horwitz AF
    The Journal of cell biology 95.2 Pt 1 (1982 Nov): 654-66.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
    Weijer CJ
    Development (Cambridge, England) 135.21 (2008 Nov): 3521-30.

    Rho kinase inhibitor Y27632 affects initial heart myofibrillogenesis in cultured chick blastoderm.
    Nakajima Y
    Developmental dynamics : an official publication of the American Association of Anatomists 236.2 (2007 Feb): 461-72.

    Perturbation of extracellular matrix prevents association of the otic primordium with the posterior rhombencephalon and inhibits subsequent invagination.
    Hilfer SR
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 48-58.

    The B cell antigen receptor controls integrin activity through Btk and PLCgamma2.
    Pals ST
    The Journal of experimental medicine 198.10 (2003 Nov 17): 1539-50.

    Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.
    Kim J
    Cell motility and the cytoskeleton 45.1 (2000 Jan): 27-41.

    Mechanism of skin morphogenesis. I. Analyses with antibodies to adhesion molecules tenascin, N-CAM, and integrin.
    Chuong CM
    Developmental biology 150.1 (1992 Mar): 82-98.

    Beta 1 integrins mediate chondrocyte interaction with type I collagen, type II collagen, and fibronectin.
    Boettiger D
    Experimental cell research 205.2 (1993 Apr): 276-85.

    Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.
    Horwitz AF
    The Journal of cell biology 100.5 (1985 May): 1528-39.

    Perturbation of cranial neural crest migration by the HNK-1 antibody.
    Bronner-Fraser M
    Developmental biology 123.2 (1987 Oct): 321-31.

    Mapping in vivo associations of cytoplasmic proteins with integrin beta 1 cytoplasmic domain mutants.
    Schwartz MA
    Molecular biology of the cell 6.2 (1995 Feb): 151-60.

    WB References
    IHC References
    IP References
    ELISA References

    A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
    Horwitz AF
    The Journal of cell biology 95.2 Pt 1 (1982 Nov): 654-66.

    FB References

    A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
    Horwitz AF
    The Journal of cell biology 95.2 Pt 1 (1982 Nov): 654-66.

    Integrin αVβ5-mediated Removal of Apoptotic Cell Debris by the Eye Lens and Its Inhibition by UV Light Exposure.
    Kantorow M
    The Journal of biological chemistry 290.51 (2015 Dec 18): 30253-66.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Bruton's tyrosine kinase and phospholipase Cgamma2 mediate chemokine-controlled B cell migration and homing.
    Spaargaren M
    Immunity 26.1 (2007 Jan): 93-104.

    The B cell antigen receptor controls integrin activity through Btk and PLCgamma2.
    Pals ST
    The Journal of experimental medicine 198.10 (2003 Nov 17): 1539-50.

    Perturbation of extracellular matrix prevents association of the otic primordium with the posterior rhombencephalon and inhibits subsequent invagination.
    Hilfer SR
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 48-58.

    The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
    Wewer UM
    The Journal of cell biology 149.5 (2000 May 29): 1143-56.

    Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.
    Kim J
    Cell motility and the cytoskeleton 45.1 (2000 Jan): 27-41.

    Embryonic neural retinal cell response to extracellular matrix proteins: developmental changes and effects of the cell substratum attachment antibody (CSAT).
    Reichardt LF
    The Journal of cell biology 104.3 (1987 Mar): 623-34.

    Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation.
    Boettiger D
    Cell 51.1 (1987 Oct 9): 51-7.

    Beta 1 integrins mediate chondrocyte interaction with type I collagen, type II collagen, and fibronectin.
    Boettiger D
    Experimental cell research 205.2 (1993 Apr): 276-85.

    Functional inhibition of retinal pigment epithelial cell-substrate adhesion with a monoclonal antibody against the beta 1 subunit of integrin.
    Grunwald GB
    Investigative ophthalmology and visual science 32.6 (1991 May): 1763-9.

    Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120-kDa protein.
    Hynes RO
    Cell regulation 2.11 (1991 Nov): 951-64.

    Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.
    Horwitz AF
    The Journal of cell biology 100.5 (1985 May): 1528-39.

    Perturbation of cranial neural crest migration by the HNK-1 antibody.
    Bronner-Fraser M
    Developmental biology 123.2 (1987 Oct): 321-31.

    Cell-surface regulation of beta 1-integrin activity on developing retinal neurons.
    Reichardt LF
    Nature 350.6313 (1991 Mar 7): 68-71.

    A role for fibronectin in the migration of avian precardiac cells. I. Dose-dependent effects of fibronectin antibody.
    Lash JW
    Developmental biology 129.2 (1988 Oct): 315-23.

    Antibodies to beta 1-integrins cause alterations of aortic vasculogenesis, in vivo.
    Little CD
    Developmental dynamics : an official publication of the American Association of Anatomists 193.1 (1992 Jan): 83-91.

    Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus.
    Wallace BG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.4 (1989 Apr): 1294-302.

    FACS References

    The B cell antigen receptor controls integrin activity through Btk and PLCgamma2.
    Pals ST
    The Journal of experimental medicine 198.10 (2003 Nov 17): 1539-50.

    Epitope Map References

    Structure/function analysis of the integrin beta 1 subunit by epitope mapping.
    Buck CA
    The Journal of cell biology 122.6 (1993 Sep): 1361-71.

    All References

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
    Horwitz AF
    The Journal of cell biology 95.2 Pt 1 (1982 Nov): 654-66.

    PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
    Weijer CJ
    Development (Cambridge, England) 135.21 (2008 Nov): 3521-30.

    Rho kinase inhibitor Y27632 affects initial heart myofibrillogenesis in cultured chick blastoderm.
    Nakajima Y
    Developmental dynamics : an official publication of the American Association of Anatomists 236.2 (2007 Feb): 461-72.

    Perturbation of extracellular matrix prevents association of the otic primordium with the posterior rhombencephalon and inhibits subsequent invagination.
    Hilfer SR
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 48-58.

    The B cell antigen receptor controls integrin activity through Btk and PLCgamma2.
    Pals ST
    The Journal of experimental medicine 198.10 (2003 Nov 17): 1539-50.

    Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.
    Kim J
    Cell motility and the cytoskeleton 45.1 (2000 Jan): 27-41.

    Mechanism of skin morphogenesis. I. Analyses with antibodies to adhesion molecules tenascin, N-CAM, and integrin.
    Chuong CM
    Developmental biology 150.1 (1992 Mar): 82-98.

    Beta 1 integrins mediate chondrocyte interaction with type I collagen, type II collagen, and fibronectin.
    Boettiger D
    Experimental cell research 205.2 (1993 Apr): 276-85.

    Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.
    Horwitz AF
    The Journal of cell biology 100.5 (1985 May): 1528-39.

    Perturbation of cranial neural crest migration by the HNK-1 antibody.
    Bronner-Fraser M
    Developmental biology 123.2 (1987 Oct): 321-31.

    Mapping in vivo associations of cytoplasmic proteins with integrin beta 1 cytoplasmic domain mutants.
    Schwartz MA
    Molecular biology of the cell 6.2 (1995 Feb): 151-60.

    Antibodies to the conserved cytoplasmic domain of the integrin beta 1 subunit react with proteins in vertebrates, invertebrates, and fungi.
    Hynes RO
    The Journal of cell biology 106.5 (1988 May): 1765-72.

    Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins.
    Languino LR
    The Journal of cell biology 166.3 (2004 Aug 2): 407-18.

    Structure/function analysis of the integrin beta 1 subunit by epitope mapping.
    Buck CA
    The Journal of cell biology 122.6 (1993 Sep): 1361-71.

    Embryonic neural retinal cell response to extracellular matrix proteins: developmental changes and effects of the cell substratum attachment antibody (CSAT).
    Reichardt LF
    The Journal of cell biology 104.3 (1987 Mar): 623-34.

    Cell-surface regulation of beta 1-integrin activity on developing retinal neurons.
    Reichardt LF
    Nature 350.6313 (1991 Mar 7): 68-71.

    Integrin (the CSAT antigen): functionality requires oligomeric integrity.
    Horwitz AF
    The Journal of cell biology 103.6 Pt 1 (1986 Dec): 2421-8.

    Integrin αVβ5-mediated Removal of Apoptotic Cell Debris by the Eye Lens and Its Inhibition by UV Light Exposure.
    Kantorow M
    The Journal of biological chemistry 290.51 (2015 Dec 18): 30253-66.

    Bruton's tyrosine kinase and phospholipase Cgamma2 mediate chemokine-controlled B cell migration and homing.
    Spaargaren M
    Immunity 26.1 (2007 Jan): 93-104.

    The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
    Wewer UM
    The Journal of cell biology 149.5 (2000 May 29): 1143-56.

    Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation.
    Boettiger D
    Cell 51.1 (1987 Oct 9): 51-7.

    Functional inhibition of retinal pigment epithelial cell-substrate adhesion with a monoclonal antibody against the beta 1 subunit of integrin.
    Grunwald GB
    Investigative ophthalmology and visual science 32.6 (1991 May): 1763-9.

    Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120-kDa protein.
    Hynes RO
    Cell regulation 2.11 (1991 Nov): 951-64.

    A role for fibronectin in the migration of avian precardiac cells. I. Dose-dependent effects of fibronectin antibody.
    Lash JW
    Developmental biology 129.2 (1988 Oct): 315-23.

    Antibodies to beta 1-integrins cause alterations of aortic vasculogenesis, in vivo.
    Little CD
    Developmental dynamics : an official publication of the American Association of Anatomists 193.1 (1992 Jan): 83-91.

    Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus.
    Wallace BG
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.4 (1989 Apr): 1294-302.

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