CSAT
Catalog Fields
Clone ID/Product Name: CSAT
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ITGB1
Ab Isotype: MIgG2b
Gene Name:
Entrez Gene ID: 374058
Clonality: Monoclonal
Immunogen: Membrane blebs from 52-hr cultured myoblasts
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP 2/0-Ag14
Epitope Mapped: Yes
Antigen Name: Integrin beta-1 (avian)
Epitope Location or Sequence: N-terminus a.a. 1-160
Alternate Antigen Name:
Deposit Date: 2/3/1995
Antigen Molecular Weight: 120 kDa and 160 kDa
Depositor: Horwitz, A.F.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Chick
Depositor Notes: The cell substrate attachment antibody (CSAT) blocks migrationof neural crest in vivo, prevents neurite outgrowth and blocks myoblast fusion and differentiation in vitro. The antigenic site recognized by CSAT is sensitive to denaturation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chick, Quail
Additional Information: In vivo injection of CSAT may be localized in frozen sections (PMID: 2944780). RRID:AB_528142
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CSAT was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product CSAT)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
28 References
Initial Publication
A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
Horwitz AF
The Journal of cell biology 95.2 Pt 1 (1982 Nov): 654-66.