V2E9

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$40.00
SKU: V2E9
View product citations for antibody V2E9 on CiteAb

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Available: 46

DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 (avian)/CD29
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: Horwitz, A.F.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Avian, Chick
Depositors Notes: For immunocytochemistry, use ethanol fixation. For western blot, proteinase inhibitors are recommended.
Antigen Molecular Weight: 88.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGB1
Immunogen: Immunoaffinity-purified integrin B1 from E11 chick embryos
Alternate Gene Names: FNRB, MDF2, MSK12
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0AG14
Epitope Location or Sequence:
Uniprot ID: P05556 
Immunogen Sequence:
Entrez Gene ID: 374058 
Additional Characterization:  
Antibody Registry ID: AB_2128055 
Additional Information: RRID:AB_2128055
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
V2E9 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product V2E9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References

    FARP2 triggers signals for Sema3A-mediated axonal repulsion.
    Kikutani H
    Nature neuroscience 8.12 (2005 Dec): 1712-9.

    All References

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes & development 21.5 (2007 Mar 1): 531-6.

    Expression and function of chicken integrin beta 1 subunit and its cytoplasmic domain mutants in mouse NIH 3T3 cells.
    Horwitz A
    The Journal of cell biology 110.1 (1990 Jan): 175-84.

    Integrins alpha(6A)beta 1 and alpha(6B)beta 1 promote different stages of chondrogenic cell differentiation.
    Tacchetti C
    The Journal of biological chemistry 277.35 (2002 Aug 30): 31612-22.

    FARP2 triggers signals for Sema3A-mediated axonal repulsion.
    Kikutani H
    Nature neuroscience 8.12 (2005 Dec): 1712-9.

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