V2E9

(0) No Reviews yet
$40.00
SKU: V2E9
View product citations for antibody V2E9 on CiteAb

In Stock

Available: 47

DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 (avian)/CD29
Hybridoma Cells Available: Yes
Antigen Species: Chick
Depositor: Horwitz, A.F.
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Virginia
Host Species: mouse
Depositors Notes: For immunocytochemistry, use ethanol fixation. For western blot, proteinase inhibitors are recommended.
Positive Tested Species Reactivity: Avian, Chick
Gene: ITGB1
Antigen Molecular Weight: 88.5 kDa
Alternate Gene Names: FNRB, MDF2, MSK12
Immunogen: Immunoaffinity-purified integrin B1 from E11 chick embryos
Clonality: Monoclonal
Myeloma Strain: SP2/0AG14
Epitope Mapped: No
Uniprot ID: P05556 
Additional Information: RRID:AB_2128055
Entrez Gene ID: 374058 
Antibody Registry ID: AB_2128055 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
V2E9 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product V2E9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References

    FARP2 triggers signals for Sema3A-mediated axonal repulsion.
    Kikutani H
    Nature neuroscience 8.12 (2005 Dec): 1712-9.

    All References

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes & development 21.5 (2007 Mar 1): 531-6.

    Expression and function of chicken integrin beta 1 subunit and its cytoplasmic domain mutants in mouse NIH 3T3 cells.
    Horwitz A
    The Journal of cell biology 110.1 (1990 Jan): 175-84.

    Integrins alpha(6A)beta 1 and alpha(6B)beta 1 promote different stages of chondrogenic cell differentiation.
    Tacchetti C
    The Journal of biological chemistry 277.35 (2002 Aug 30): 31612-22.

    FARP2 triggers signals for Sema3A-mediated axonal repulsion.
    Kikutani H
    Nature neuroscience 8.12 (2005 Dec): 1712-9.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review