P2C62C4

(0) No Reviews yet
$40.00
SKU: P2C62C4
View product citations for antibody P2C62C4 on CiteAb

In Stock

Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-6, avian
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Horwitz, A.F.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Avian
Depositors Notes: This antibody has been generated against denatured protein; it immunoblots well and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but doesn't precipitate the native receptor. Stains chicken embryo cryosections.
Antigen Molecular Weight: Predicted: 119 kDa; Apparent: 130 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGA6
Immunogen: Denatured integrin alpha subunits purified from adult chicken brain tissue.
Alternate Gene Names: VLA-6
Alternate Antibody Name: alpha6C6
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P26007 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396226 
Additional Characterization:  
Antibody Registry ID: AB_528301 
Additional Information: P2C62C4 recognizes both uncleaved (proform) and cleaved forms of alpha-6 integrin.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2C62C4 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product P2C62C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication

    Developmentally regulated expression of alpha 6 integrin in avian embryos.
    Horwitz AF
    Development (Cambridge, England) 115.1 (1992 May): 197-211.

    IF References

    Developmentally regulated expression of alpha 6 integrin in avian embryos.
    Horwitz AF
    Development (Cambridge, England) 115.1 (1992 May): 197-211.

    Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility.
    Condic ML
    The Journal of cell biology 167.3 (2004 Nov 8): 545-54.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    Cellular responses of embryonic hyaline cartilage to experimental wounding in vitro.
    Archer CW
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 18.1 (2000 Jan): 25-34.

    Retroviral transfer of antisense integrin alpha6 or alpha8 sequences results in laminar redistribution or clonal cell death in developing brain.
    Galileo DS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.17 (1998 Sep 1): 6928-38.

    Characterization of chicken epidermal dendritic cells.
    Magyar A
    Immunology 119.2 (2006 Oct): 278-88.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Progressive bundling of fibrillin microfibrils into oxytalan fibers in the chick presumptive dermis.
    Isokawa K
    Anatomical record (Hoboken, N.J. : 2007) 296.1 (2013 Jan): 71-8.

    Developmentally regulated expression of alpha 6 integrin in avian embryos.
    Horwitz AF
    Development (Cambridge, England) 115.1 (1992 May): 197-211.

    WB References

    Developmentally regulated expression of alpha 6 integrin in avian embryos.
    Horwitz AF
    Development (Cambridge, England) 115.1 (1992 May): 197-211.

    Insulin-like growth factor-I stimulates neurogenesis in chick retina by regulating expression of the alpha 6 integrin subunit.
    Rodríguez-Tébar A
    Development (Cambridge, England) 122.8 (1996 Aug): 2497-506.

    Role for alpha 6 integrin during lens development: Evidence for signaling through IGF-1R and ERK.
    Menko AS
    Developmental dynamics : an official publication of the American Association of Anatomists 223.2 (2002 Mar): 273-84.

    IHC References
    IP References

    Developmentally regulated expression of alpha 6 integrin in avian embryos.
    Horwitz AF
    Development (Cambridge, England) 115.1 (1992 May): 197-211.

    Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility.
    Condic ML
    The Journal of cell biology 167.3 (2004 Nov 8): 545-54.

    Role for alpha 6 integrin during lens development: Evidence for signaling through IGF-1R and ERK.
    Menko AS
    Developmental dynamics : an official publication of the American Association of Anatomists 223.2 (2002 Mar): 273-84.

    FACS References

    Retroviral transfer of antisense integrin alpha6 or alpha8 sequences results in laminar redistribution or clonal cell death in developing brain.
    Galileo DS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.17 (1998 Sep 1): 6928-38.

    All References

    Establishment of autologous embryonic stem cells derived from preantral follicle culture and oocyte parthenogenesis.
    Lim JM
    Fertility and sterility 90.5 (2008 Nov): 1910-20.

    Developmentally regulated expression of alpha 6 integrin in avian embryos.
    Horwitz AF
    Development (Cambridge, England) 115.1 (1992 May): 197-211.

    Cranial neural crest recycle surface integrins in a substratum-dependent manner to promote rapid motility.
    Condic ML
    The Journal of cell biology 167.3 (2004 Nov 8): 545-54.

    RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
    Sheng G
    Nature cell biology 10.7 (2008 Jul): 765-75.

    Cellular responses of embryonic hyaline cartilage to experimental wounding in vitro.
    Archer CW
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society 18.1 (2000 Jan): 25-34.

    Retroviral transfer of antisense integrin alpha6 or alpha8 sequences results in laminar redistribution or clonal cell death in developing brain.
    Galileo DS
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.17 (1998 Sep 1): 6928-38.

    Characterization of chicken epidermal dendritic cells.
    Magyar A
    Immunology 119.2 (2006 Oct): 278-88.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Progressive bundling of fibrillin microfibrils into oxytalan fibers in the chick presumptive dermis.
    Isokawa K
    Anatomical record (Hoboken, N.J. : 2007) 296.1 (2013 Jan): 71-8.

    Insulin-like growth factor-I stimulates neurogenesis in chick retina by regulating expression of the alpha 6 integrin subunit.
    Rodríguez-Tébar A
    Development (Cambridge, England) 122.8 (1996 Aug): 2497-506.

    Role for alpha 6 integrin during lens development: Evidence for signaling through IGF-1R and ERK.
    Menko AS
    Developmental dynamics : an official publication of the American Association of Anatomists 223.2 (2002 Mar): 273-84.

    Differential expression of two cell adhesion molecules, Ephrin-A5 and Integrin alpha6, during cranial neurulation in the chick embryo.
    Schoenwolf GC
    Developmental neuroscience 25.5 (2003 Sep-Oct): 357-65.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review