P2C62C4
Catalog Fields
Antigen: integrin alpha-6, avian
Available to For-Profits: Yes
Antigen Species: Chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Horwitz, A.F.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian
Depositors Institution: University of Virginia
Antigen Molecular Weight: Predicted: 119 kDa; Apparent: 130 kDa
Depositors Notes: This antibody has been generated against denatured protein; it immunoblots well and immunoprecipitates the monomeric alpha subunit after the dimeric receptor has first been denatured, but doesn't precipitate the native receptor. Stains chicken embryo cryosections.
Immunogen: Denatured integrin alpha subunits purified from adult chicken brain tissue.
Gene: ITGA6
Alternate Antibody Name: alpha6C6
Alternate Gene Names: VLA-6
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: P26007
Epitope Location or Sequence:
Entrez Gene ID: 396226
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528301
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: P2C62C4 recognizes both uncleaved (proform) and cleaved forms of alpha-6 integrin.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2C62C4 was deposited to the DSHB by Horwitz, A.F. (DSHB Hybridoma Product P2C62C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Developmentally regulated expression of alpha 6 integrin in avian embryos.
Horwitz AF
Development (Cambridge, England) 115.1 (1992 May): 197-211.