Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Universitaetsklinikim Wuerzburg
Positive Tested Species Reactivity: Drosophila, Mosquito
Depositors Notes: This antibody specifically labels presynaptic active zones.
Antigen Molecular Weight: 201.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Head homogenate
Alternate Gene Names: CG42344
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: C-terminal aa 1227-1740
Uniprot ID: A1Z7V1
Entrez Gene ID: 35977
Antibody Registry ID: AB_2314866
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
nc82 was deposited to the DSHB by Buchner, E. (DSHB Hybridoma Product nc82)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References