nc82

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$40.00
SKU: nc82
View product citations for antibody nc82 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Bruchpilot
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E.
Isotype: MIgG1, kappa light chain
Depositors Institution: Universitaetsklinikim Wuerzburg
Host Species: mouse
Depositors Notes: This antibody specifically labels presynaptic active zones.
Positive Tested Species Reactivity: Drosophila, Mosquito
Gene: Brp
Antigen Molecular Weight: 201.6 kDa
Alternate Gene Names: CG42344
Immunogen: Head homogenate
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Uniprot ID: A1Z7V1 
Epitope Location or Sequence: C-terminal aa 1227-1740
Entrez Gene ID: 35977 
Antibody Registry ID: AB_2314866 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
nc82 was deposited to the DSHB by Buchner, E. (DSHB Hybridoma Product nc82)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

46 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Contribution of Orb2A stability in regulated amyloid-like oligomerization of Drosophila Orb2.
    Si K
    PLoS biology 12.2 (2014 Feb): e1001786.

    The Ly6 neurotoxin-like molecule target of wit regulates spontaneous neurotransmitter release at the developing neuromuscular junction in Drosophila.
    Marqués G
    Developmental neurobiology 72.12 (2012 Dec): 1541-58.

    Epigenetic regulation of olfactory receptor gene expression by the Myb-MuvB/dREAM complex.
    Ray A
    Genes & development 26.22 (2012 Nov 15): 2483-98.

    The nonsense-mediated decay pathway maintains synapse architecture and synaptic vesicle cycle efficacy.
    Broadie K
    Journal of cell science 123.Pt 19 (2010 Oct 1): 3303-15.

    Sialyltransferase regulates nervous system function in Drosophila.
    Panin VM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.18 (2010 May 5): 6466-76.

    Writing memories with light-addressable reinforcement circuitry.
    Miesenböck G
    Cell 139.2 (2009 Oct 16): 405-15.

    Genetic dissection of neural circuit anatomy underlying feeding behavior in Drosophila: distinct classes of hugin-expressing neurons.
    Pankratz MJ
    The Journal of comparative neurology 502.5 (2007 Jun 10): 848-56.

    Fragile X Mental Retardation Protein Requirements in Activity-Dependent Critical Period Neural Circuit Refinement.
    Broadie K
    Current biology : CB 27.15 (2017 Aug 7): 2318-2330.e3.

    Dissection of the <i>Drosophila</i> neuropeptide F circuit using a high-throughput two-choice assay.
    Heberlein U
    Proceedings of the National Academy of Sciences of the United States of America 114.38 (2017 Sep 19): E8091-E8099.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Identification of DmTTLL5 as a Major Tubulin Glutamylase in the Drosophila Nervous System.
    Soustelle L
    Scientific reports 7.1 (2017 Nov 24): 16254.

    Identification of DmTTLL5 as a Major Tubulin Glutamylase in the Drosophila Nervous System.
    Soustelle L
    Scientific reports 7.1 (2017 Nov 24): 16254.

    Digital in vivo 3D atlas of the antennal lobe of Drosophila melanogaster.
    Sachse S
    The Journal of comparative neurology 523.3 (2015 Feb 15): 530-44.

    Neuroarchitecture and neuroanatomy of the Drosophila central complex: A GAL4-based dissection of protocerebral bridge neurons and circuits.
    Rubin GM
    The Journal of comparative neurology 523.7 (2015 May 1): 997-1037.

    Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5754-71.

    How to escape from Haller's rule: Olfactory system complexity in small and large Trichogramma evanescens parasitic wasps.
    Smid HM
    The Journal of comparative neurology 524.9 (2016 Jun 15): 1876-91.

    A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the <i>Drosophila</i> motor system.
    Yao CK
    eLife 8. (2019 Jul 18): .

    Organization of projection neurons and local neurons of the primary auditory center in the fruit fly Drosophila melanogaster.
    Kamikouchi A
    The Journal of comparative neurology 524.6 (2016 Apr 15): 1099-164.

    Organization of projection neurons and local neurons of the primary auditory center in the fruit fly Drosophila melanogaster.
    Kamikouchi A
    The Journal of comparative neurology 524.6 (2016 Apr 15): 1099-164.

    Distinct homeostatic modulations stabilize reduced postsynaptic receptivity in response to presynaptic DLK signaling.
    Dickman D
    Nature communications 9.1 (2018 May 10): 1856.

    Fan-Shaped Body Neurons in the Drosophila Brain Regulate Both Innate and Conditioned Nociceptive Avoidance.
    Zhong Y
    Cell reports 24.6 (2018 Aug 7): 1573-1584.

    Levels of Par-1 kinase determine the localization of Bruchpilot at the Drosophila neuromuscular junction synapses.
    Wairkar YP
    Scientific reports 8.1 (2018 Oct 31): 16099.

    Dysfunction of <i>GRAP</i>, encoding the GRB2-related adaptor protein, is linked to sensorineural hearing loss.
    Tekin M
    Proceedings of the National Academy of Sciences of the United States of America 116.4 (2019 Jan 22): 1347-1352.

    Neural Evolution of Context-Dependent Fly Song.
    Stern DL
    Current biology : CB 29.7 (2019 Apr 1): 1089-1099.e7.

    Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila.
    Vanderzalm PJ
    Developmental neurobiology 79.4 (2019 Apr): 335-349.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

    Developmental expression of human tau in Drosophila melanogaster glial cells induces motor deficits and disrupts maintenance of PNS axonal integrity, without affecting synapse formation.
    Colodner KJ
    PloS one 14.12 (2019): e0226380.

    WB References

    Complexin controls spontaneous and evoked neurotransmitter release by regulating the timing and properties of synaptotagmin activity.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.50 (2012 Dec 12): 18234-45.

    A Drosophila DEG/ENaC subunit functions specifically in gustatory neurons required for male courtship behavior.
    Pikielny CW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.13 (2012 Mar 28): 4665-74.

    Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways.
    Soller M
    Genome biology 9.4 (2008 Apr 17): R73.

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    IHC References

    External and circadian inputs modulate synaptic protein expression in the visual system of Drosophila melanogaster.
    Pyza EM
    Frontiers in physiology 5. (2014): 102.

    Identification of novel vibration- and deflection-sensitive neuronal subgroups in Johnston's organ of the fruit fly.
    Kamikouchi A
    Frontiers in physiology 5. (2014): 179.

    Insulin-producing cells regulate the sexual receptivity through the painless TRP channel in Drosophila virgin females.
    Kitamoto T
    PloS one 9.2 (2014): e88175.

    Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.30 (2013 Jul 24): 12352-63.

    Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.30 (2013 Jul 24): 12352-63.

    Parallel pathways for cross-modal memory retrieval in Drosophila.
    Guo A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.20 (2013 May 15): 8784-93.

    Single dopaminergic neurons that modulate aggression in Drosophila.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 110.15 (2013 Apr 9): 6151-6.

    Lobula-specific visual projection neurons are involved in perception of motion-defined second-order motion in Drosophila.
    Guo A
    The Journal of experimental biology 216.Pt 3 (2013 Feb 1): 524-34.

    The kinesin-3, unc-104 regulates dendrite morphogenesis and synaptic development in Drosophila.
    Rasse TM
    Genetics 195.1 (2013 Sep): 59-72.

    Parallel Transformation of Tactile Signals in Central Circuits of Drosophila.
    Wilson RI
    Cell 164.5 (2016 Feb 25): 1046-59.

    RNA-Seq Transcriptome Analysis of Direction-Selective T4/T5 Neurons in Drosophila.
    Borst A
    PloS one 11.9 (2016): e0163986.

    Aging Drosophila melanogaster display altered pre- and postsynaptic ultrastructure at adult neuromuscular junctions.
    Neuser K
    The Journal of comparative neurology 523.16 (2015 Nov 1): 2457-75.

    Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster.
    Hansson BS
    The Journal of comparative neurology 524.9 (2016 Jun 15): 1920-56.

    Learning a Spatial Task by Trial and Error in Drosophila.
    Yang CH
    Current biology : CB 29.15 (2019 Aug 5): 2517-2525.e5.

    cindr, the Drosophila Homolog of the CD2AP Alzheimer's Disease Risk Gene, Is Required for Synaptic Transmission and Proteostasis.
    Shulman JM
    Cell reports 28.7 (2019 Aug 13): 1799-1813.e5.

    Epitope Map References

    Maturation of active zone assembly by Drosophila Bruchpilot.
    Sigrist SJ
    The Journal of cell biology 186.1 (2009 Jul 13): 129-45.

    All References

    External and circadian inputs modulate synaptic protein expression in the visual system of Drosophila melanogaster.
    Pyza EM
    Frontiers in physiology 5. (2014): 102.

    Identification of novel vibration- and deflection-sensitive neuronal subgroups in Johnston's organ of the fruit fly.
    Kamikouchi A
    Frontiers in physiology 5. (2014): 179.

    Insulin-producing cells regulate the sexual receptivity through the painless TRP channel in Drosophila virgin females.
    Kitamoto T
    PloS one 9.2 (2014): e88175.

    Brain tumor regulates neuromuscular synapse growth and endocytosis in Drosophila by suppressing mad expression.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.30 (2013 Jul 24): 12352-63.

    Parallel pathways for cross-modal memory retrieval in Drosophila.
    Guo A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.20 (2013 May 15): 8784-93.

    Single dopaminergic neurons that modulate aggression in Drosophila.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 110.15 (2013 Apr 9): 6151-6.

    Lobula-specific visual projection neurons are involved in perception of motion-defined second-order motion in Drosophila.
    Guo A
    The Journal of experimental biology 216.Pt 3 (2013 Feb 1): 524-34.

    The kinesin-3, unc-104 regulates dendrite morphogenesis and synaptic development in Drosophila.
    Rasse TM
    Genetics 195.1 (2013 Sep): 59-72.

    Parallel Transformation of Tactile Signals in Central Circuits of Drosophila.
    Wilson RI
    Cell 164.5 (2016 Feb 25): 1046-59.

    RNA-Seq Transcriptome Analysis of Direction-Selective T4/T5 Neurons in Drosophila.
    Borst A
    PloS one 11.9 (2016): e0163986.

    Aging Drosophila melanogaster display altered pre- and postsynaptic ultrastructure at adult neuromuscular junctions.
    Neuser K
    The Journal of comparative neurology 523.16 (2015 Nov 1): 2457-75.

    Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster.
    Hansson BS
    The Journal of comparative neurology 524.9 (2016 Jun 15): 1920-56.

    Learning a Spatial Task by Trial and Error in Drosophila.
    Yang CH
    Current biology : CB 29.15 (2019 Aug 5): 2517-2525.e5.

    cindr, the Drosophila Homolog of the CD2AP Alzheimer's Disease Risk Gene, Is Required for Synaptic Transmission and Proteostasis.
    Shulman JM
    Cell reports 28.7 (2019 Aug 13): 1799-1813.e5.

    Contribution of Orb2A stability in regulated amyloid-like oligomerization of Drosophila Orb2.
    Si K
    PLoS biology 12.2 (2014 Feb): e1001786.

    The Ly6 neurotoxin-like molecule target of wit regulates spontaneous neurotransmitter release at the developing neuromuscular junction in Drosophila.
    Marqués G
    Developmental neurobiology 72.12 (2012 Dec): 1541-58.

    Epigenetic regulation of olfactory receptor gene expression by the Myb-MuvB/dREAM complex.
    Ray A
    Genes & development 26.22 (2012 Nov 15): 2483-98.

    The nonsense-mediated decay pathway maintains synapse architecture and synaptic vesicle cycle efficacy.
    Broadie K
    Journal of cell science 123.Pt 19 (2010 Oct 1): 3303-15.

    Sialyltransferase regulates nervous system function in Drosophila.
    Panin VM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.18 (2010 May 5): 6466-76.

    Writing memories with light-addressable reinforcement circuitry.
    Miesenböck G
    Cell 139.2 (2009 Oct 16): 405-15.

    Genetic dissection of neural circuit anatomy underlying feeding behavior in Drosophila: distinct classes of hugin-expressing neurons.
    Pankratz MJ
    The Journal of comparative neurology 502.5 (2007 Jun 10): 848-56.

    Fragile X Mental Retardation Protein Requirements in Activity-Dependent Critical Period Neural Circuit Refinement.
    Broadie K
    Current biology : CB 27.15 (2017 Aug 7): 2318-2330.e3.

    Dissection of the <i>Drosophila</i> neuropeptide F circuit using a high-throughput two-choice assay.
    Heberlein U
    Proceedings of the National Academy of Sciences of the United States of America 114.38 (2017 Sep 19): E8091-E8099.

    Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments.
    Hartenstein V
    The Journal of comparative neurology 526.1 (2018 Jan 1): 33-58.

    Identification of DmTTLL5 as a Major Tubulin Glutamylase in the Drosophila Nervous System.
    Soustelle L
    Scientific reports 7.1 (2017 Nov 24): 16254.

    Digital in vivo 3D atlas of the antennal lobe of Drosophila melanogaster.
    Sachse S
    The Journal of comparative neurology 523.3 (2015 Feb 15): 530-44.

    Neuroarchitecture and neuroanatomy of the Drosophila central complex: A GAL4-based dissection of protocerebral bridge neurons and circuits.
    Rubin GM
    The Journal of comparative neurology 523.7 (2015 May 1): 997-1037.

    Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5754-71.

    How to escape from Haller's rule: Olfactory system complexity in small and large Trichogramma evanescens parasitic wasps.
    Smid HM
    The Journal of comparative neurology 524.9 (2016 Jun 15): 1876-91.

    A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the <i>Drosophila</i> motor system.
    Yao CK
    eLife 8. (2019 Jul 18): .

    Organization of projection neurons and local neurons of the primary auditory center in the fruit fly Drosophila melanogaster.
    Kamikouchi A
    The Journal of comparative neurology 524.6 (2016 Apr 15): 1099-164.

    Distinct homeostatic modulations stabilize reduced postsynaptic receptivity in response to presynaptic DLK signaling.
    Dickman D
    Nature communications 9.1 (2018 May 10): 1856.

    Fan-Shaped Body Neurons in the Drosophila Brain Regulate Both Innate and Conditioned Nociceptive Avoidance.
    Zhong Y
    Cell reports 24.6 (2018 Aug 7): 1573-1584.

    Levels of Par-1 kinase determine the localization of Bruchpilot at the Drosophila neuromuscular junction synapses.
    Wairkar YP
    Scientific reports 8.1 (2018 Oct 31): 16099.

    Dysfunction of <i>GRAP</i>, encoding the GRB2-related adaptor protein, is linked to sensorineural hearing loss.
    Tekin M
    Proceedings of the National Academy of Sciences of the United States of America 116.4 (2019 Jan 22): 1347-1352.

    Neural Evolution of Context-Dependent Fly Song.
    Stern DL
    Current biology : CB 29.7 (2019 Apr 1): 1089-1099.e7.

    Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila.
    Vanderzalm PJ
    Developmental neurobiology 79.4 (2019 Apr): 335-349.

    A small number of cholinergic neurons mediate hyperaggression in female <i>Drosophila</i>.
    Kravitz EA
    Proceedings of the National Academy of Sciences of the United States of America 116.34 (2019 Aug 20): 17029-17038.

    Developmental expression of human tau in Drosophila melanogaster glial cells induces motor deficits and disrupts maintenance of PNS axonal integrity, without affecting synapse formation.
    Colodner KJ
    PloS one 14.12 (2019): e0226380.

    Complexin controls spontaneous and evoked neurotransmitter release by regulating the timing and properties of synaptotagmin activity.
    Littleton JT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.50 (2012 Dec 12): 18234-45.

    A Drosophila DEG/ENaC subunit functions specifically in gustatory neurons required for male courtship behavior.
    Pikielny CW
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.13 (2012 Mar 28): 4665-74.

    Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways.
    Soller M
    Genome biology 9.4 (2008 Apr 17): R73.

    Using Drosophila as an integrated model to study mild repetitive traumatic brain injury.
    Ratliff EP
    Scientific reports 6. (2016 May 4): 25252.

    Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila.
    Buchner E
    Neuron 49.6 (2006 Mar 16): 833-44.

    Maturation of active zone assembly by Drosophila Bruchpilot.
    Sigrist SJ
    The Journal of cell biology 186.1 (2009 Jul 13): 129-45.

    Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.
    Katanaev VL
    Development (Cambridge, England) 141.17 (2014 Sep): 3399-409.

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