P4C10
Catalog Fields
Antigen: integrin beta-1 (CD29), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Predicted Species Reactivity:
Gene: ITGB1
Immunogen: Human fibrosarcoma HT-1080 cells
Alternate Gene Names: CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA
Alternate Antibody Name: CD29
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218)
Uniprot ID: P05556
Immunogen Sequence: Full length protein
Entrez Gene ID: 3688
Antibody Registry ID: AB_2619600
Additional Information: While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4C10 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
18 References
Initial Publication
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Carter WG
The Journal of cell biology 105.4 (1987 Oct): 1873-84.