P4C10

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$40.00
SKU: P4C10
View product citations for antibody P4C10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 (CD29), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGB1
Immunogen: Human fibrosarcoma HT-1080 cells
Alternate Gene Names: CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA
Alternate Antibody Name: CD29
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218)
Uniprot ID: P05556 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3688 
Additional Characterization:  
Antibody Registry ID: AB_2619600 
Additional Information: While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4C10 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    ELISA References
    FB References

    Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    Carter WG
    The Journal of cell biology 105.4 (1987 Oct): 1873-84.

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    A ligand-independent integrin β1 mechanosensory complex guides spindle orientation.
    Skourides PA
    Nature communications 7. (2016 Mar 8): 10899.

    Requirement of the integrin beta 3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen.
    Cheresh DA
    The Journal of cell biology 117.5 (1992 Jun): 1101-7.

    Identification of surface proteins mediating adherence of CD11/CD18-deficient lymphoblastoid cells to cultured human endothelium.
    Harlan JM
    The Journal of clinical investigation 85.6 (1990 Jun): 2019-22.

    Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
    Cheresh DA
    The Journal of cell biology 113.4 (1991 May): 919-29.

    Activation-dependent recognition by hematopoietic cells of the LDV sequence in the V region of fibronectin.
    Kovach NL
    The Journal of cell biology 116.2 (1992 Jan): 489-97.

    Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
    Carter WG
    The Journal of cell biology 121.5 (1993 Jun): 1141-52.

    Coordinate role for cell surface chondroitin sulfate proteoglycan and alpha 4 beta 1 integrin in mediating melanoma cell adhesion to fibronectin.
    McCarthy JB
    The Journal of cell biology 118.2 (1992 Jul): 431-44.

    Human B lymphocytes define an alternative mechanism of adhesion to fibronectin. The interaction of the alpha 4 beta 1 integrin with the LHGPEILDVPST sequence of the type III connecting segment is sufficient to promote cell attachment.
    Ferreira OC Jr
    Journal of immunology (Baltimore, Md. : 1950) 144.9 (1990 May 1): 3361-6.

    Integrin expression and function on human osteoblast-like cells.
    Simmons PJ
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 12.8 (1997 Aug): 1189-97.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    FACS References
    Epitope Map References

    Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies.
    Puzon W
    The Journal of biological chemistry 268.23 (1993 Aug 15): 17597-601.

    All References

    Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes.
    Wayner EA
    The Journal of cell biology 111.6 Pt 2 (1990 Dec): 3141-54.

    Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.
    Carter WG
    The Journal of cell biology 121.5 (1993 Jun): 1141-52.

    SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion.
    Coppolino MG
    Molecular biology of the cell 25.13 (2014 Jul 1): 2061-70.

    Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells.
    Coppolino MG
    The Journal of biological chemistry 292.39 (2017 Sep 29): 16199-16210.

    Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    Carter WG
    The Journal of cell biology 105.4 (1987 Oct): 1873-84.

    Identification of surface proteins mediating adherence of CD11/CD18-deficient lymphoblastoid cells to cultured human endothelium.
    Harlan JM
    The Journal of clinical investigation 85.6 (1990 Jun): 2019-22.

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    A ligand-independent integrin β1 mechanosensory complex guides spindle orientation.
    Skourides PA
    Nature communications 7. (2016 Mar 8): 10899.

    Requirement of the integrin beta 3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen.
    Cheresh DA
    The Journal of cell biology 117.5 (1992 Jun): 1101-7.

    Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
    Cheresh DA
    The Journal of cell biology 113.4 (1991 May): 919-29.

    Activation-dependent recognition by hematopoietic cells of the LDV sequence in the V region of fibronectin.
    Kovach NL
    The Journal of cell biology 116.2 (1992 Jan): 489-97.

    Coordinate role for cell surface chondroitin sulfate proteoglycan and alpha 4 beta 1 integrin in mediating melanoma cell adhesion to fibronectin.
    McCarthy JB
    The Journal of cell biology 118.2 (1992 Jul): 431-44.

    Human B lymphocytes define an alternative mechanism of adhesion to fibronectin. The interaction of the alpha 4 beta 1 integrin with the LHGPEILDVPST sequence of the type III connecting segment is sufficient to promote cell attachment.
    Ferreira OC Jr
    Journal of immunology (Baltimore, Md. : 1950) 144.9 (1990 May 1): 3361-6.

    Integrin expression and function on human osteoblast-like cells.
    Simmons PJ
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 12.8 (1997 Aug): 1189-97.

    Integrin α-3 ß-1's central role in breast cancer, melanoma and glioblastoma cell aggregation revealed by antibodies with blocking activity.
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies.
    Puzon W
    The Journal of biological chemistry 268.23 (1993 Aug 15): 17597-601.

    Development of monoclonal antibodies to integrin receptors.
    Hoffstrom BG
    Methods in enzymology 426. (2007): 117-53.

    Cross-talk of integrin alpha3beta1 and tissue factor in cell migration.
    Ruf W
    Molecular biology of the cell 15.10 (2004 Oct): 4416-25.

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