P4C10
Catalog Fields
Clone ID/Product Name: P4C10
Available to For-Profits: Yes
Alternate Antibody Name: CD29
Gene Symbol: ITGB1
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2619600
Uniprot ID: P05556
Entrez Gene ID: 3688
Clonality: Monoclonal
Immunogen: Human fibrosarcoma HT-1080 cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: integrin beta-1 (CD29), human
Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218)
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot