Antibody Name: P4C10
Antigen:integrin beta-1 (CD29), human
Catalog Fields
Identity
Clone ID / Antibody Name: P4C10
Alternate Antibody Name: CD29
Antigen Name: integrin beta-1 (CD29), human
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: ITGB1
Entrez Gene ID: 3688
Antigen Sequence: —
Antigen Species: Human
Antigen Mol. Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Uniprot ID: P05556
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: Mouse IgG1
Myeloma Strain: NS-1/FOX-NY
Antibody Registry / RRID: AB_2619600
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Human fibrosarcoma HT-1080 cells
Immunogen Sequence: Full length protein
Epitope Mapped: Yes
Epitope Location / Seq: NKGEVFNELVGK (a.a. 207-218)
Clone: —
Additional Info: While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Human
Predicted Reactivity: —
Depositor
Depositor Name: E.A. Wayner / W.G. Carter
Institution: Fred Hutchinson Cancer Research Center
Deposit Date: October 11, 2011
Depositor Notes: P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Recommended Applications
ELISA
FACS
FFPE
Function Blocking
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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