P4C10
Catalog Fields
Product Name/ID: P4C10
Available to For-Profits: Yes
Alternate Antibody Name: CD29
Gene Name: ITGB1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619600
Uniprot ID: P05556
Entrez Gene ID: 3688
Clonality: Monoclonal
Immunogen: Human fibrosarcoma HT-1080 cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: integrin beta-1 (CD29), human
Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218)
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4C10 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
18 References
Initial Publication
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Carter WG
The Journal of cell biology 105.4 (1987 Oct): 1873-84.