YAP1 8J19

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SKU: YAP1 8J19

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DSHB Data Sheet

Catalog Fields

Product Name/ID: YAP1 8J19
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Yap1
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2619554 
Uniprot ID: P46938 
RRID:  
Entrez Gene ID: 22601 
Clonality: Monoclonal
Immunogen: YAP1 peptide
Clone:
Immunogen Sequence: Peptide
Myeloma Strain:
Epitope Mapped: No
Antigen Name: YAP1 (YAP65 / Yes-associated protein 1)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/14/2013
Antigen Molecular Weight: Predicted: 51 and 52 kDa; Apparent 65 kDa
Depositor: Pu, W.
Antigen Sequence:
Depositor Institution: Boston Children's Hospital
Antigen Species: Mouse
Depositor Notes: This mAb is not recommended for western blotting as it gives many nonspecific bands
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information: Transcriptional coactivator YAP1
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
YAP1 8J19 was deposited to the DSHB by Pu, W. (DSHB Hybridoma Product YAP1 8J19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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