Antigen: YAP1 (YAP65 / Yes-associated protein 1)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Pu, W.
Isotype: MIgG2a, kappa light chain
Host Species: mouse
Depositors Institution: Children's Hospital, Boston
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: This mAb is not recommended for western blotting as it gives many nonspecific bands
Predicted Species Reactivity:
Immunogen: YAP1 peptide
Alternate Gene Names: Yap, Yap65, Yki, Yorkie
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Epitope Location or Sequence:
Uniprot ID: P46938
Immunogen Sequence: Peptide
Entrez Gene ID: 22601
Antibody Registry ID: AB_2619554
Additional Information: Transcriptional coactivator YAP1
Recommended Applications: Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
YAP1 8J19 was deposited to the DSHB by Pu, W. (DSHB Hybridoma Product YAP1 8J19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.