YAP1 8J19

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$45.00 to $425.00
SKU: YAP1 8J19
View product citations for antibody YAP1 8J19 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: YAP1 (YAP65 / Yes-associated protein 1)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Pu, W.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston Children's Hospital
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: This mAb is not recommended for western blotting as it gives many nonspecific bands
Antigen Molecular Weight: Predicted: 51 and 52 kDa; Apparent 65 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Yap1
Immunogen: YAP1 peptide
Alternate Gene Names: Yap, Yap65, Yki, Yorkie
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P46938 
Immunogen Sequence: Peptide
Entrez Gene ID: 22601 
Additional Characterization:  
Antibody Registry ID: AB_2619554 
Additional Information: Transcriptional coactivator YAP1
Recommended Applications: Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
YAP1 8J19 was deposited to the DSHB by Pu, W. (DSHB Hybridoma Product YAP1 8J19)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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