zns-2

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SKU: zns-2-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: zns-2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531907 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Adult brain membranes. Boosts: adult CNS membranes
Clone:
Immunogen Sequence:
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antigen Name: nervous system marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/30/1996
Antigen Molecular Weight:
Depositor: Trevarrow, B.
Antigen Sequence:
Depositor Institution: University of Oregon, Institute of Neuroscience
Antigen Species: zebrafish
Depositor Notes: Doesn't work on Xenopus, betta fish, chicken, shark or Haplochromis burtoni. Works well on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat) the surface of neural cells, especially in neuropil and fiber tracts. Doesn't work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
zns-2 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product zns-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References

    A transient population of neurons pioneers the olfactory pathway in the zebrafish.
    Westerfield M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.21 (1998 Nov 1): 8919-27.

    Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish.
    Faillace MP
    Synapse (New York, N.Y.) 63.4 (2009 Apr): 291-307.

    Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio.
    Whitlock KE
    Developmental dynamics : an official publication of the American Association of Anatomists 234.1 (2005 Sep): 229-42.

    Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis.
    Blader P
    Development (Cambridge, England) 138.21 (2011 Nov): 4753-62.

    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A transient population of neurons pioneers the olfactory pathway in the zebrafish.
    Westerfield M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.21 (1998 Nov 1): 8919-27.

    Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system.
    Yoshihara Y
    Development (Cambridge, England) 132.6 (2005 Mar): 1283-93.

    Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish.
    Watanabe S
    Developmental biology 255.1 (2003 Mar 1): 113-27.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A transient population of neurons pioneers the olfactory pathway in the zebrafish.
    Westerfield M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.21 (1998 Nov 1): 8919-27.

    Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system.
    Yoshihara Y
    Development (Cambridge, England) 132.6 (2005 Mar): 1283-93.

    Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish.
    Watanabe S
    Developmental biology 255.1 (2003 Mar 1): 113-27.

    Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish.
    Faillace MP
    Synapse (New York, N.Y.) 63.4 (2009 Apr): 291-307.

    Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio.
    Whitlock KE
    Developmental dynamics : an official publication of the American Association of Anatomists 234.1 (2005 Sep): 229-42.

    Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis.
    Blader P
    Development (Cambridge, England) 138.21 (2011 Nov): 4753-62.

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