zns-2

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$40.00
SKU: zns-2-s
View product citations for antibody zns-2 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: nervous system marker
Hybridoma Cells Available: No
Antigen Species: zebrafish
Depositor: Trevarrow, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Neuroscience, University of Oregon
Positive Tested Species Reactivity: Zebrafish
Depositors Notes: Doesn't work on Xenopus, betta fish, chicken, shark or Haplochromis burtoni. Works well on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat) the surface of neural cells, especially in neuropil and fiber tracts. Doesn't work for western blotting.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Adult brain membranes. Boosts: adult CNS membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531907 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
zns-2 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product zns-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References

    A transient population of neurons pioneers the olfactory pathway in the zebrafish.
    Westerfield M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.21 (1998 Nov 1): 8919-27.

    Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish.
    Faillace MP
    Synapse (New York, N.Y.) 63.4 (2009 Apr): 291-307.

    Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio.
    Whitlock KE
    Developmental dynamics : an official publication of the American Association of Anatomists 234.1 (2005 Sep): 229-42.

    Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis.
    Blader P
    Development (Cambridge, England) 138.21 (2011 Nov): 4753-62.

    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A transient population of neurons pioneers the olfactory pathway in the zebrafish.
    Westerfield M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.21 (1998 Nov 1): 8919-27.

    Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system.
    Yoshihara Y
    Development (Cambridge, England) 132.6 (2005 Mar): 1283-93.

    Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish.
    Watanabe S
    Developmental biology 255.1 (2003 Mar 1): 113-27.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A transient population of neurons pioneers the olfactory pathway in the zebrafish.
    Westerfield M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.21 (1998 Nov 1): 8919-27.

    Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system.
    Yoshihara Y
    Development (Cambridge, England) 132.6 (2005 Mar): 1283-93.

    Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish.
    Watanabe S
    Developmental biology 255.1 (2003 Mar 1): 113-27.

    Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish.
    Faillace MP
    Synapse (New York, N.Y.) 63.4 (2009 Apr): 291-307.

    Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio.
    Whitlock KE
    Developmental dynamics : an official publication of the American Association of Anatomists 234.1 (2005 Sep): 229-42.

    Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis.
    Blader P
    Development (Cambridge, England) 138.21 (2011 Nov): 4753-62.

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