Antigen: Synaptotagmin 2, synaptic marker
Depositor: Trevarrow, B.
Antigen Species: Zebrafish
Depositors Institution: Institute of Neuroscience, University of Oregon
Isotype: MIgG2a, kappa light chain
Depositors Notes: Works well in zebrafish embryos/larva or adults. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat or paraffin) the cytoplasm in neurons, early processes and neuropil regions.
Host Species: mouse
Positive Tested Species Reactivity: Amphibian, Chicken, Human, Mouse, Rat, Zebrafish
Antigen Molecular Weight: Expected: 48 kDa; Apparent: 60 kDa
Epitope Location or Sequence: Although syt2 is heavily glycosylated, the znp-1 antibody recognizes a protein epitope.
Immunogen: Fixed embryos. Boosts: adult spinal cord
Immunogen Sequence: Full length protein
Myeloma Strain: SP 2/0
Additional Information: Recognizes primary motor neurones.
Uniprot ID: F1R0Q0
Entrez Gene ID: 567877
Antibody Registry ID: AB_2315626
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
znp-1 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product znp-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References