znp-1

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$40.00
SKU: znp-1
View product citations for antibody znp-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Synaptotagmin 2, synaptic marker
Hybridoma Cells Available: No
Antigen Species: Zebrafish
Depositor: Trevarrow, B.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Neuroscience, University of Oregon
Positive Tested Species Reactivity: Amphibian, Chicken, Human, Mouse, Rat, Zebrafish
Depositors Notes: Works well in zebrafish embryos/larva or adults. Stains (4% paraformaldehyde, 4ºC, O/N; cryostat or paraffin) the cytoplasm in neurons, early processes and neuropil regions.
Antigen Molecular Weight: Expected: 48 kDa; Apparent: 60 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: syt2a
Immunogen: Fixed embryos. Boosts: adult spinal cord
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence: Although syt2 is heavily glycosylated, the znp-1 antibody recognizes a protein epitope.
Uniprot ID: F1R0Q0 
Immunogen Sequence: Full length protein
Entrez Gene ID: 567877 
Additional Characterization:  
Antibody Registry ID: AB_2315626 
Additional Information: Recognizes primary motor neurones.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
znp-1 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product znp-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References
    WB References
    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis.
    Cheng SH
    Toxicological sciences : an official journal of the Society of Toxicology 73.1 (2003 May): 149-59.

    Synaptotagmins I and II in the developing rat auditory brainstem: Synaptotagmin I is transiently expressed in glutamate-releasing immature inhibitory terminals.
    Gillespie DC
    The Journal of comparative neurology 519.12 (2011 Aug 15): 2417-33.

    IP References

    Synaptotagmin I and II are present in distinct subsets of central synapses.
    Sanes JR
    The Journal of comparative neurology 503.2 (2007 Jul 10): 280-96.

    FFPE References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Epitope Map References

    Synaptotagmin I and II are present in distinct subsets of central synapses.
    Sanes JR
    The Journal of comparative neurology 503.2 (2007 Jul 10): 280-96.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Cadmium affects muscle type development and axon growth in zebrafish embryonic somitogenesis.
    Cheng SH
    Toxicological sciences : an official journal of the Society of Toxicology 73.1 (2003 May): 149-59.

    Synaptotagmins I and II in the developing rat auditory brainstem: Synaptotagmin I is transiently expressed in glutamate-releasing immature inhibitory terminals.
    Gillespie DC
    The Journal of comparative neurology 519.12 (2011 Aug 15): 2417-33.

    Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity.
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases.
    Burgess SM
    Scientific reports 6. (2016 Jul 18): 29946.

    Loss of function of glucocerebrosidase GBA2 is responsible for motor neuron defects in hereditary spastic paraplegia.
    Stevanin G
    American journal of human genetics 92.2 (2013 Feb 7): 238-44.

    Synaptotagmin I and II are present in distinct subsets of central synapses.
    Sanes JR
    The Journal of comparative neurology 503.2 (2007 Jul 10): 280-96.

    Ethanol exposure during gastrulation alters neuronal morphology and behavior in zebrafish.
    Ali DW
    Neurotoxicology and teratology 48. (2015 Mar-Apr): 18-27.

    Effects of BDE-209 contaminated sediments on zebrafish development and potential implications to human health.
    Raldúa D
    Environment international 63. (2014 Feb): 216-23.

    Effects of 4-methylbenzylidene camphor (4-MBC) on neuronal and muscular development in zebrafish (Danio rerio) embryos.
    Cheng SH
    Environmental science and pollution research international 23.9 (2016 May): 8275-85.

    Granulin knock out zebrafish lack frontotemporal lobar degeneration and neuronal ceroid lipofuscinosis pathology.
    Schmid B
    PloS one 10.3 (2015): e0118956.

    Activation of α2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish.
    Svoboda KR
    The European journal of neuroscience 40.1 (2014 Jul): 2225-40.

    Mesenchymal Stem Cells Therapy Improved the Streptozotocin-Induced Behavioral and Hippocampal Impairment in Rats.
    Reggiani PC
    Molecular neurobiology 57.2 (2020 Feb): 600-615.

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