zn-12
Catalog Fields
Antigen: neuronal cell surface marker (HNK-1 carbohydrate epitope)
Hybridoma Cells Available: No
Antigen Species: zebrafish
Depositor: Trevarrow, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Oregon, Institute of Neuroscience
Positive Tested Species Reactivity: Chicken, Fish, Shark, Xenopus, Zebrafish
Depositors Notes: Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface of many but not all neurons, fibers and neuropil, muscle cells and receptor cells.
Predicted Species Reactivity:
Gene:
Immunogen: Adult brain membranes. Boosts: adult CNS membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP 2/0
Epitope Location or Sequence: HNK-1 carbohydrate epitope
Additional Characterization: https://zfin.org/zf_info/zfbook/zfmabs.html
Antibody Registry ID: AB_531908
Additional Information: Zn-12 recognizes both sulfated and non-sulfated HNK-1 epitopes.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
zn-12 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product zn-12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
18 References
Initial Publication
Organization of hindbrain segments in the zebrafish embryo.
Kimmel CB
Neuron 4.5 (1990 May): 669-79.