zn-12

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$40.00
SKU: zn-12-s
View product citations for antibody zn-12 on CiteAb

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Available: 185

DSHB Data Sheet

Catalog Fields

Antigen: neuronal cell surface marker (HNK-1 carbohydrate epitope)
Hybridoma Cells Available: No
Antigen Species: zebrafish
Depositor: Trevarrow, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Neuroscience, University of Oregon
Positive Tested Species Reactivity: Chicken, Fish, Shark, Xenopus, Zebrafish
Depositors Notes: Works on zebrafish embryos/larva and adults. Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cell surface of many but not all neurons, fibers and neuropil, muscle cells and receptor cells.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Adult brain membranes. Boosts: adult CNS membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP 2/0
Epitope Location or Sequence: HNK-1 carbohydrate epitope
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization: https://zfin.org/zf_info/zfbook/zfmabs.html 
Antibody Registry ID: AB_531908 
Additional Information: Zn-12 recognizes both sulfated and non-sulfated HNK-1 epitopes.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
zn-12 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product zn-12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References

    Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish.
    Chan J
    Nature chemical biology 2.5 (2006 May): 265-73.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    Distribution and morphology of cholinergic cells in the branchial epithelium of zebrafish (Danio rerio).
    Jonz MG
    Cell and tissue research 367.2 (2017 Feb): 169-179.

    New details of the neural architecture of the salmonid adipose fin.
    Buckland-Nicks JA
    Journal of fish biology 89.4 (2016 Oct): 1991-2003.

    Mitogenic action of hypoxia upon cutaneous neuroepithelial cells in developing zebrafish.
    Jonz MG
    Developmental neurobiology 77.6 (2017 Jun): 789-801.

    Regional innervation of the heart in the goldfish, Carassius auratus: a confocal microscopy study.
    Smith FM
    The Journal of comparative neurology 522.2 (2014 Feb 1): 456-78.

    Early phosphorylation of MARCKS at Ser25 in migrating precursor cells and differentiating peripheral neurons.
    Zolessi FR
    Neuroscience letters 544. (2013 Jun 7): 5-9.

    Regional innervation of the heart in the goldfish, Carassius auratus: a confocal microscopy study.
    Smith FM
    The Journal of comparative neurology 522.2 (2014 Feb 1): 456-78.

    Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms.
    Cepko CL
    The Journal of comparative neurology 523.11 (2015 Aug 1): 1639-63.

    Excessive activation of ionotropic glutamate receptors induces apoptotic hair-cell death independent of afferent and efferent innervation.
    Sheets L
    Scientific reports 7. (2017 Jan 23): 41102.

    WB References
    IHC References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Primary neurons that express the L2/HNK-1 carbohydrate during early development in the zebrafish.
    Kimmel CB
    Development (Cambridge, England) 110.2 (1990 Oct): 491-504.

    MRF4 gene expression in Xenopus embryos and aneural myofibers.
    Hinterberger T
    Developmental dynamics : an official publication of the American Association of Anatomists 226.3 (2003 Mar): 551-4.

    Novel expression patterns of Pax3/Pax7 in early trunk neural crest and its melanocyte and non-melanocyte lineages in amniote embryos.
    Domínguez L
    Pigment cell research 18.4 (2005 Aug): 243-51.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    Structure, development, and functional morphology of the cement gland of the giant danio, Devario malabaricus.
    Lafontant PJ
    Developmental dynamics : an official publication of the American Association of Anatomists 248.11 (2019 Nov): 1155-1174.

    Regeneration of the gill filaments and replacement of serotonergic neuroepithelial cells in adult zebrafish (Danio rerio).
    Jonz MG
    Respiratory physiology & neurobiology 274. (2020 Mar): 103366.

    IP References

    Primary neurons that express the L2/HNK-1 carbohydrate during early development in the zebrafish.
    Kimmel CB
    Development (Cambridge, England) 110.2 (1990 Oct): 491-504.

    Epitope Map References

    Primary neurons that express the L2/HNK-1 carbohydrate during early development in the zebrafish.
    Kimmel CB
    Development (Cambridge, England) 110.2 (1990 Oct): 491-504.

    All References

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Primary neurons that express the L2/HNK-1 carbohydrate during early development in the zebrafish.
    Kimmel CB
    Development (Cambridge, England) 110.2 (1990 Oct): 491-504.

    MRF4 gene expression in Xenopus embryos and aneural myofibers.
    Hinterberger T
    Developmental dynamics : an official publication of the American Association of Anatomists 226.3 (2003 Mar): 551-4.

    Novel expression patterns of Pax3/Pax7 in early trunk neural crest and its melanocyte and non-melanocyte lineages in amniote embryos.
    Domínguez L
    Pigment cell research 18.4 (2005 Aug): 243-51.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    Structure, development, and functional morphology of the cement gland of the giant danio, Devario malabaricus.
    Lafontant PJ
    Developmental dynamics : an official publication of the American Association of Anatomists 248.11 (2019 Nov): 1155-1174.

    Regeneration of the gill filaments and replacement of serotonergic neuroepithelial cells in adult zebrafish (Danio rerio).
    Jonz MG
    Respiratory physiology & neurobiology 274. (2020 Mar): 103366.

    Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish.
    Chan J
    Nature chemical biology 2.5 (2006 May): 265-73.

    Distribution and morphology of cholinergic cells in the branchial epithelium of zebrafish (Danio rerio).
    Jonz MG
    Cell and tissue research 367.2 (2017 Feb): 169-179.

    New details of the neural architecture of the salmonid adipose fin.
    Buckland-Nicks JA
    Journal of fish biology 89.4 (2016 Oct): 1991-2003.

    Mitogenic action of hypoxia upon cutaneous neuroepithelial cells in developing zebrafish.
    Jonz MG
    Developmental neurobiology 77.6 (2017 Jun): 789-801.

    Regional innervation of the heart in the goldfish, Carassius auratus: a confocal microscopy study.
    Smith FM
    The Journal of comparative neurology 522.2 (2014 Feb 1): 456-78.

    Early phosphorylation of MARCKS at Ser25 in migrating precursor cells and differentiating peripheral neurons.
    Zolessi FR
    Neuroscience letters 544. (2013 Jun 7): 5-9.

    Vesicular stomatitis virus enables gene transfer and transsynaptic tracing in a wide range of organisms.
    Cepko CL
    The Journal of comparative neurology 523.11 (2015 Aug 1): 1639-63.

    Excessive activation of ionotropic glutamate receptors induces apoptotic hair-cell death independent of afferent and efferent innervation.
    Sheets L
    Scientific reports 7. (2017 Jan 23): 41102.

    Activation of α2A-containing nicotinic acetylcholine receptors mediates nicotine-induced motor output in embryonic zebrafish.
    Svoboda KR
    The European journal of neuroscience 40.1 (2014 Jul): 2225-40.

    The Adhesion Molecule-Characteristic HNK-1 Carbohydrate Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish.
    Schachner M
    Molecular neurobiology 54.5 (2017 Jul): 3253-3263.

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