zn-1

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$40.00
SKU: zn-1-s
View product citations for antibody zn-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neuronal marker (cytoplasmic)
Hybridoma Cells Available: No
Antigen Species: zebrafish
Depositor: Trevarrow, B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Neuroscience, University of Oregon
Positive Tested Species Reactivity: Axolotl, Zebrafish
Depositors Notes: Doesn’t work on Xenopus, betta fish, trout, medaka, shark, chicken or Haplochromis burtoni. Works well on zebrafish ambryos/larva (2d-4d). Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cytoplasm of all neurons and hair cells.
Antigen Molecular Weight: Apparent: 43 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Zebrafish embryo membranes (2 days old)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531909 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
zn-1 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product zn-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References
    WB References
    IHC References

    Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo.
    Westerfield M
    Development (Cambridge, England) 103.1 (1988 May): 49-58.

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Selective labeling of sensory hair cells and neurons in auditory, vestibular, and lateral line systems by a monoclonal antibody.
    Trevarrow B
    The Journal of comparative neurology 301.2 (1990 Nov 8): 162-70.

    Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish.
    Okamoto H
    Developmental dynamics : an official publication of the American Association of Anatomists 199.1 (1994 Jan): 1-11.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Development of segmentation in zebrafish.
    Trevarrow B
    Development (Cambridge, England) 104 Suppl. (1988): 197-207.

    Characterisation of neuronal and glial populations of the visual system during zebrafish lifespan.
    Arévalo R
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 29.4 (2011 Jun): 441-9.

    All References

    Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo.
    Westerfield M
    Development (Cambridge, England) 103.1 (1988 May): 49-58.

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Selective labeling of sensory hair cells and neurons in auditory, vestibular, and lateral line systems by a monoclonal antibody.
    Trevarrow B
    The Journal of comparative neurology 301.2 (1990 Nov 8): 162-70.

    Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish.
    Okamoto H
    Developmental dynamics : an official publication of the American Association of Anatomists 199.1 (1994 Jan): 1-11.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Development of segmentation in zebrafish.
    Trevarrow B
    Development (Cambridge, England) 104 Suppl. (1988): 197-207.

    Characterisation of neuronal and glial populations of the visual system during zebrafish lifespan.
    Arévalo R
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 29.4 (2011 Jun): 441-9.

    Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity.
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
    Matthews G
    Neural development 5. (2010 Sep 1): 24.

    Morphology and cell type heterogeneities of the inner ear epithelia in adult and juvenile zebrafish (Danio rerio).
    Malicki JJ
    The Journal of comparative neurology 438.2 (2001 Sep 17): 173-90.

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