zn-1

(0)No Reviews yet
$50.00
QuantityPrice
1 – 19$50.00
20 – 49$35.00
50 – 99$32.00
100 +$30.00
SKU: zn-1-s

In Stock

Available: 37

DSHB Data Sheet

Catalog Fields

Product Name/ID: zn-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531909 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Zebrafish embryo membranes (2 days old)
Clone:
Immunogen Sequence:
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antigen Name: neuronal marker (cytoplasmic)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/30/1996
Antigen Molecular Weight: Apparent: 43 kDa
Depositor: Trevarrow, B.
Antigen Sequence:
Depositor Institution: University of Oregon, Institute of Neuroscience
Antigen Species: zebrafish
Depositor Notes: Doesn’t work on Xenopus, betta fish, trout, medaka, shark, chicken or Haplochromis burtoni. Works well on zebrafish ambryos/larva (2d-4d). Stains (4% paraformaldehyde, 4ºC, O/N, cryostat or wholemount) the cytoplasm of all neurons and hair cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Axolotl, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
zn-1 was deposited to the DSHB by Trevarrow, B. (DSHB Hybridoma Product zn-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    IF References
    WB References
    IHC References

    Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo.
    Westerfield M
    Development (Cambridge, England) 103.1 (1988 May): 49-58.

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Selective labeling of sensory hair cells and neurons in auditory, vestibular, and lateral line systems by a monoclonal antibody.
    Trevarrow B
    The Journal of comparative neurology 301.2 (1990 Nov 8): 162-70.

    Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish.
    Okamoto H
    Developmental dynamics : an official publication of the American Association of Anatomists 199.1 (1994 Jan): 1-11.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Development of segmentation in zebrafish.
    Trevarrow B
    Development (Cambridge, England) 104 Suppl. (1988): 197-207.

    Characterisation of neuronal and glial populations of the visual system during zebrafish lifespan.
    Arévalo R
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 29.4 (2011 Jun): 441-9.

    All References

    Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo.
    Westerfield M
    Development (Cambridge, England) 103.1 (1988 May): 49-58.

    Organization of hindbrain segments in the zebrafish embryo.
    Kimmel CB
    Neuron 4.5 (1990 May): 669-79.

    Selective labeling of sensory hair cells and neurons in auditory, vestibular, and lateral line systems by a monoclonal antibody.
    Trevarrow B
    The Journal of comparative neurology 301.2 (1990 Nov 8): 162-70.

    Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish.
    Okamoto H
    Developmental dynamics : an official publication of the American Association of Anatomists 199.1 (1994 Jan): 1-11.

    Midline signaling in the primordium of the zebrafish anterior central nervous system.
    Kimmel CB
    Proceedings of the National Academy of Sciences of the United States of America 91.6 (1994 Mar 15): 2061-5.

    A homeobox gene essential for zebrafish notochord development.
    Kimelman D
    Nature 378.6553 (1995 Nov 9): 150-7.

    Development of segmentation in zebrafish.
    Trevarrow B
    Development (Cambridge, England) 104 Suppl. (1988): 197-207.

    Characterisation of neuronal and glial populations of the visual system during zebrafish lifespan.
    Arévalo R
    International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 29.4 (2011 Jun): 441-9.

    Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity.
    Eisen JS
    Development (Cambridge, England) 133.11 (2006 Jun): 2137-47.

    Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
    Matthews G
    Neural development 5. (2010 Sep 1): 24.

    Morphology and cell type heterogeneities of the inner ear epithelia in adult and juvenile zebrafish (Danio rerio).
    Malicki JJ
    The Journal of comparative neurology 438.2 (2001 Sep 17): 173-90.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review