1E8
Catalog Fields
Antigen: Schwann cell marker, P(o) (avian)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Frank, E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Tufts Medical School, Department of Physiology
Positive Tested Species Reactivity: Chicken, Planaria, Quail
Depositors Notes: Western blotting and immunohistochemistry can be described in Neuron 7, 831-844, (1991).
Antigen Molecular Weight: 30 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene:
Immunogen: dorsal root ganglia
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation NS1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2078498
Additional Information: Immunization through I.P. used freund's adjuvant. The immunogen origin was chicken embryo with the developmental state embryonic day E16-E18. RRID:AB_2078498
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1E8 was deposited to the DSHB by Frank, E. (DSHB Hybridoma Product 1E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
P0 is an early marker of the Schwann cell lineage in chickens.
Brackenbury R
Neuron 7.5 (1991 Nov): 831-44.