Catalog Fields
Antigen: Schwann cell marker, P(o) (avian)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Frank, E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Tufts Medical School, Department of Physiology
Positive Tested Species Reactivity: Chicken, Planaria, Quail
Depositors Notes: Western blotting and immunohistochemistry can be described in Neuron 7, 831-844, (1991).
Predicted Species Reactivity:
Gene:
Immunogen: dorsal root ganglia
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Designation NS1
Epitope Location or Sequence:
Antibody Registry ID: AB_2078498
Additional Information: Immunization through I.P. used freund's adjuvant. The immunogen origin was chicken embryo with the developmental state embryonic day E16-E18. RRID:AB_2078498
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1E8 was deposited to the DSHB by Frank, E. (DSHB Hybridoma Product 1E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
P0 is an early marker of the Schwann cell lineage in chickens.
Brackenbury R
Neuron 7.5 (1991 Nov): 831-44.