3A10

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$40.00
SKU: 3A10
View product citations for antibody 3A10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neurofilament-associated, Mauthner cells
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Fish, Gecko, Human, Mouse, Planaria, Quail, Rat, Shark, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes a neurofilament-associated antigen. The antibody labels a subset of hindbrain spinal cord projecting neurons such as Mauthner neurons.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chick nervous tissue from ventral spinal cord/cyclophosphamide treatment/assorted nervous tissue
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531874 
Additional Information: 3A10 recognizes a conformational epitope assosciated with the TRSPXP sequence that is shared by neurofilament-associated antigens in the developing rat brain. See PUBMED #10050030.
Recommended Applications: FFPE
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3A10 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 3A10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

66 References

  • Initial Publication
  • IF References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Axon guidance and the patterning of neuronal projections in vertebrates.
    Jessell TM
    Science (New York, N.Y.) 242.4879 (1988 Nov 4): 692-9.

    IF References

    Identification of initially appearing glycine-immunoreactive neurons in the embryonic zebrafish brain.
    Hatta K
    Developmental neurobiology 74.6 (2014 Jun): 616-32.

    Growth hormone and retinal ganglion cell function: QNR/D cells as an experimental model.
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Axon guidance effects of classical morphogens Shh and BMP7 in the hypothalamo-pituitary system.
    Placzek M
    Neuroscience letters 562. (2014 Mar 6): 108-13.

    MicroRNA-9 promotes the switch from early-born to late-born motor neuron populations by regulating Onecut transcription factor expression.
    Huber AB
    Developmental biology 386.2 (2014 Feb 15): 358-70.

    Mutation of 3-hydroxy-3-methylglutaryl CoA synthase I reveals requirements for isoprenoid and cholesterol synthesis in oligodendrocyte migration arrest, axon wrapping, and myelin gene expression.
    Appel B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.9 (2014 Feb 26): 3402-12.

    A subset of chicken statoacoustic ganglion neurites are repelled by Slit1 and Slit2.
    Fekete DM
    Hearing research 310. (2014 Apr): 1-12.

    NMDA receptors on zebrafish Mauthner cells require CaMKII-α for normal development.
    Ali DW
    Developmental neurobiology 75.2 (2015 Feb): 145-62.

    Amigo adhesion protein regulates development of neural circuits in zebrafish brain.
    Rauvala H
    The Journal of biological chemistry 289.29 (2014 Jul 18): 19958-75.

    Valproic acid silencing of ascl1b/Ascl1 results in the failure of serotonergic differentiation in a zebrafish model of fetal valproate syndrome.
    Briscoe J
    Disease models and mechanisms 7.1 (2014 Jan): 107-17.

    Retinoic acid induced-1 (Rai1) regulates craniofacial and brain development in Xenopus.
    Dickinson AJ
    Mechanisms of development 133. (2014 Aug): 91-104.

    Tar DNA-binding protein-43 (TDP-43) regulates axon growth in vitro and in vivo.
    Guthrie S
    Neurobiology of disease 65. (2014 May): 25-34.

    Knockdown of Lingo1b protein promotes myelination and oligodendrocyte differentiation in zebrafish.
    Hu B
    Experimental neurology 251. (2014 Jan): 72-83.

    Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability.
    Ribera AB
    Neural development 9. (2014 Aug 22): 19.

    prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements.
    Sagerström CG
    Developmental biology 390.2 (2014 Jun 15): 247-60.

    The function of fin rays as proprioceptive sensors in fish.
    Hale ME
    Nature communications 4. (2013): 1729.

    A critical role for STIM1 in filopodial calcium entry and axon guidance.
    Ming GL
    Molecular brain 6. (2013 Dec 1): 51.

    Cultures of astroglial cells derived from brain of adult cichlid fish.
    Tiedemann K
    Journal of neuroscience methods 212.2 (2013 Jan 30): 269-75.

    Simple methods for generating neural, bone and endodermal cell types from chick embryonic stem cells.
    Stern CD
    Stem cell research 10.1 (2013 Jan): 20-8.

    Developmental expression of the axonal glycoprotein TAG-1: differential regulation by central and peripheral neurons in vitro.
    Jessell TM
    Development (Cambridge, England) 112.1 (1991 May): 51-67.

    Role of Chd7 in zebrafish: a model for CHARGE syndrome.
    Moldovan F
    PloS one 7.2 (2012): e31650.

    Functions of thioredoxin1 in brain development and in response to environmental chemicals in zebrafish embryos.
    Strähle U
    Toxicology letters 314. (2019 Oct 10): 43-52.

    IHC References

    Secondary neurulation of human embryos: morphological changes and the expression of neuronal antigens.
    Wang KC
    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery 30.1 (2014 Jan): 73-82.

    A subset of chicken statoacoustic ganglion neurites are repelled by Slit1 and Slit2.
    Fekete DM
    Hearing research 310. (2014 Apr): 1-12.

    On the maxillary nerve.
    Kuratani S
    Journal of morphology 275.1 (2014 Jan): 17-38.

    Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish.
    Gong Y
    Disease models and mechanisms 6.2 (2013 Mar): 404-13.

    Complementary expression of EphA7 and SCO-spondin during posterior commissure development.
    Caprile T
    Frontiers in neuroanatomy 8. (2014): 49.

    Fgf10 expression patterns in the developing chick inner ear.
    Hidalgo-Sánchez M
    The Journal of comparative neurology 521.5 (2013 Apr 1): 1136-64.

    Role of Chd7 in zebrafish: a model for CHARGE syndrome.
    Moldovan F
    PloS one 7.2 (2012): e31650.

    Individual axons regulate the myelinating potential of single oligodendrocytes in vivo.
    Lyons DA
    Development (Cambridge, England) 138.20 (2011 Oct): 4443-50.

    Multiple mechanisms mediate motor neuron migration in the zebrafish hindbrain.
    Chandrasekhar A
    Developmental neurobiology 70.2 (2010 Feb): 87-99.

    Potential roles of Arnt2 in zebrafish larval development.
    Peterson RE
    Zebrafish 6.1 (2009 Mar): 79-91.

    The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish.
    Chandrasekhar A
    Developmental biology 325.2 (2009 Jan 15): 363-73.

    Zebrafish bashful/laminin-alpha 1 mutants exhibit multiple axon guidance defects.
    Halloran MC
    Developmental dynamics : an official publication of the American Association of Anatomists 235.1 (2006 Jan): 213-24.

    A nonsense mutation in the gene encoding a zebrafish myosin VI isoform causes defects in hair-cell mechanotransduction.
    Hudspeth AJ
    Proceedings of the National Academy of Sciences of the United States of America 101.35 (2004 Aug 31): 13056-61.

    PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching.
    Okamoto H
    Development (Cambridge, England) 131.15 (2004 Aug): 3705-15.

    Mutations in deadly seven/notch1a reveal developmental plasticity in the escape response circuit.
    Beattie CE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.22 (2003 Sep 3): 8159-66.

    parachute/n-cadherin is required for morphogenesis and maintained integrity of the zebrafish neural tube.
    Bally-Cuif L
    Development (Cambridge, England) 129.14 (2002 Jul): 3281-94.

    The unique axon trajectory of the accessory nerve is determined by intrinsic properties of the neural tube in the avian embryo.
    Huang R
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 205. (2016 May): 85-9.

    Neuronal labeling patterns in the spinal cord of adult transgenic Zebrafish.
    Drapeau P
    Developmental neurobiology 76.6 (2016 Jun): 642-60.

    Cyp1B1 expression patterns in the developing chick inner ear.
    Hidalgo-Sánchez M
    Developmental dynamics : an official publication of the American Association of Anatomists . (2019 Aug 9): .

    FFPE References

    AP-2 and HNK-1 define distinct populations of cranial neural crest cells.
    Golden JA
    Orthodontics and craniofacial research 6.4 (2003 Nov): 210-9.

    All References

    Secondary neurulation of human embryos: morphological changes and the expression of neuronal antigens.
    Wang KC
    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery 30.1 (2014 Jan): 73-82.

    A subset of chicken statoacoustic ganglion neurites are repelled by Slit1 and Slit2.
    Fekete DM
    Hearing research 310. (2014 Apr): 1-12.

    On the maxillary nerve.
    Kuratani S
    Journal of morphology 275.1 (2014 Jan): 17-38.

    Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish.
    Gong Y
    Disease models and mechanisms 6.2 (2013 Mar): 404-13.

    Complementary expression of EphA7 and SCO-spondin during posterior commissure development.
    Caprile T
    Frontiers in neuroanatomy 8. (2014): 49.

    Fgf10 expression patterns in the developing chick inner ear.
    Hidalgo-Sánchez M
    The Journal of comparative neurology 521.5 (2013 Apr 1): 1136-64.

    Role of Chd7 in zebrafish: a model for CHARGE syndrome.
    Moldovan F
    PloS one 7.2 (2012): e31650.

    Individual axons regulate the myelinating potential of single oligodendrocytes in vivo.
    Lyons DA
    Development (Cambridge, England) 138.20 (2011 Oct): 4443-50.

    Multiple mechanisms mediate motor neuron migration in the zebrafish hindbrain.
    Chandrasekhar A
    Developmental neurobiology 70.2 (2010 Feb): 87-99.

    Potential roles of Arnt2 in zebrafish larval development.
    Peterson RE
    Zebrafish 6.1 (2009 Mar): 79-91.

    The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish.
    Chandrasekhar A
    Developmental biology 325.2 (2009 Jan 15): 363-73.

    Zebrafish bashful/laminin-alpha 1 mutants exhibit multiple axon guidance defects.
    Halloran MC
    Developmental dynamics : an official publication of the American Association of Anatomists 235.1 (2006 Jan): 213-24.

    A nonsense mutation in the gene encoding a zebrafish myosin VI isoform causes defects in hair-cell mechanotransduction.
    Hudspeth AJ
    Proceedings of the National Academy of Sciences of the United States of America 101.35 (2004 Aug 31): 13056-61.

    PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching.
    Okamoto H
    Development (Cambridge, England) 131.15 (2004 Aug): 3705-15.

    Mutations in deadly seven/notch1a reveal developmental plasticity in the escape response circuit.
    Beattie CE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.22 (2003 Sep 3): 8159-66.

    parachute/n-cadherin is required for morphogenesis and maintained integrity of the zebrafish neural tube.
    Bally-Cuif L
    Development (Cambridge, England) 129.14 (2002 Jul): 3281-94.

    The unique axon trajectory of the accessory nerve is determined by intrinsic properties of the neural tube in the avian embryo.
    Huang R
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 205. (2016 May): 85-9.

    Neuronal labeling patterns in the spinal cord of adult transgenic Zebrafish.
    Drapeau P
    Developmental neurobiology 76.6 (2016 Jun): 642-60.

    Cyp1B1 expression patterns in the developing chick inner ear.
    Hidalgo-Sánchez M
    Developmental dynamics : an official publication of the American Association of Anatomists . (2019 Aug 9): .

    Identification of initially appearing glycine-immunoreactive neurons in the embryonic zebrafish brain.
    Hatta K
    Developmental neurobiology 74.6 (2014 Jun): 616-32.

    Growth hormone and retinal ganglion cell function: QNR/D cells as an experimental model.
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Axon guidance effects of classical morphogens Shh and BMP7 in the hypothalamo-pituitary system.
    Placzek M
    Neuroscience letters 562. (2014 Mar 6): 108-13.

    MicroRNA-9 promotes the switch from early-born to late-born motor neuron populations by regulating Onecut transcription factor expression.
    Huber AB
    Developmental biology 386.2 (2014 Feb 15): 358-70.

    Mutation of 3-hydroxy-3-methylglutaryl CoA synthase I reveals requirements for isoprenoid and cholesterol synthesis in oligodendrocyte migration arrest, axon wrapping, and myelin gene expression.
    Appel B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.9 (2014 Feb 26): 3402-12.

    NMDA receptors on zebrafish Mauthner cells require CaMKII-α for normal development.
    Ali DW
    Developmental neurobiology 75.2 (2015 Feb): 145-62.

    Amigo adhesion protein regulates development of neural circuits in zebrafish brain.
    Rauvala H
    The Journal of biological chemistry 289.29 (2014 Jul 18): 19958-75.

    Valproic acid silencing of ascl1b/Ascl1 results in the failure of serotonergic differentiation in a zebrafish model of fetal valproate syndrome.
    Briscoe J
    Disease models and mechanisms 7.1 (2014 Jan): 107-17.

    Retinoic acid induced-1 (Rai1) regulates craniofacial and brain development in Xenopus.
    Dickinson AJ
    Mechanisms of development 133. (2014 Aug): 91-104.

    Tar DNA-binding protein-43 (TDP-43) regulates axon growth in vitro and in vivo.
    Guthrie S
    Neurobiology of disease 65. (2014 May): 25-34.

    Knockdown of Lingo1b protein promotes myelination and oligodendrocyte differentiation in zebrafish.
    Hu B
    Experimental neurology 251. (2014 Jan): 72-83.

    Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability.
    Ribera AB
    Neural development 9. (2014 Aug 22): 19.

    prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements.
    Sagerström CG
    Developmental biology 390.2 (2014 Jun 15): 247-60.

    The function of fin rays as proprioceptive sensors in fish.
    Hale ME
    Nature communications 4. (2013): 1729.

    A critical role for STIM1 in filopodial calcium entry and axon guidance.
    Ming GL
    Molecular brain 6. (2013 Dec 1): 51.

    Cultures of astroglial cells derived from brain of adult cichlid fish.
    Tiedemann K
    Journal of neuroscience methods 212.2 (2013 Jan 30): 269-75.

    Simple methods for generating neural, bone and endodermal cell types from chick embryonic stem cells.
    Stern CD
    Stem cell research 10.1 (2013 Jan): 20-8.

    Developmental expression of the axonal glycoprotein TAG-1: differential regulation by central and peripheral neurons in vitro.
    Jessell TM
    Development (Cambridge, England) 112.1 (1991 May): 51-67.

    Functions of thioredoxin1 in brain development and in response to environmental chemicals in zebrafish embryos.
    Strähle U
    Toxicology letters 314. (2019 Oct 10): 43-52.

    AP-2 and HNK-1 define distinct populations of cranial neural crest cells.
    Golden JA
    Orthodontics and craniofacial research 6.4 (2003 Nov): 210-9.

    Axon guidance and the patterning of neuronal projections in vertebrates.
    Jessell TM
    Science (New York, N.Y.) 242.4879 (1988 Nov 4): 692-9.

    Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression.
    Wood MD
    Biotechnology and bioengineering 111.9 (2014 Sep): 1886-94.

    Mutant human FUS Is ubiquitously mislocalized and generates persistent stress granules in primary cultured transgenic zebrafish cells.
    Cole NJ
    PloS one 9.6 (2014): e90572.

    The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons.
    Boeckers TM
    Neural development 8. (2013 Sep 17): 18.

    Neuropilin-1 biases dendrite polarization in the retina.
    McFarlane S
    Development (Cambridge, England) 140.14 (2013 Jul): 2933-41.

    Zebrafish rgs4 is essential for motility and axonogenesis mediated by Akt signaling.
    Shen CJ
    Cellular and molecular life sciences : CMLS 70.5 (2013 Mar): 935-50.

    MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.
    Jiang HY
    Genetics and molecular research : GMR 13.2 (2014 Jun 9): 4187-201.

    Primary neuron culture for nerve growth and axon guidance studies in zebrafish (Danio rerio).
    Henley JR
    PloS one 8.3 (2013): e57539.

    Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo.
    Hirata H
    Genes to cells : devoted to molecular and cellular mechanisms 18.3 (2013 Mar): 211-24.

    Targeted mutation of the gene encoding prion protein in zebrafish reveals a conserved role in neuron excitability.
    Allison WT
    Neurobiology of disease 55. (2013 Jul): 11-25.

    Coexpression of auxiliary Kvβ2 subunits with Kv1.1 channels is required for developmental acquisition of unique firing properties of zebrafish Mauthner cells.
    Oda Y
    Journal of neurophysiology 111.6 (2014 Mar): 1153-64.

    Targeted germ line disruptions reveal general and species-specific roles for paralog group 1 hox genes in zebrafish.
    Sagerström CG
    BMC developmental biology 14. (2014 Jun 5): 25.

    A novel role for Pax6 in the segmental organization of the hindbrain.
    Sela-Donenfeld D
    Development (Cambridge, England) 140.10 (2013 May): 2190-202.

    Occipital somites guide motor axons of the accessory nerve in the avian embryo.
    Huang R
    Neuroscience 246. (2013 Aug 29): 22-7.

    Plexin A3 and turnout regulate motor axonal branch morphogenesis in zebrafish.
    Granato M
    PloS one 8.1 (2013): e54071.

    Axon guidance effect of classical morphogens Shh and BMP7 in the hypothalamo-pituitary system.
    Xu H
    Neuroscience letters 553. (2013 Oct 11): 104-9.

    Direct and indirect roles of Fgf3 and Fgf10 in innervation and vascularisation of the vertebrate hypothalamic neurohypophysis.
    Placzek M
    Development (Cambridge, England) 140.5 (2013 Mar): 1111-22.

    Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system.
    Tessier-Lavigne M
    Cell 87.6 (1996 Dec 13): 1001-14.

    Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Ventrally located commissural neurons express the GABAergic phenotype in developing rat spinal cord.
    Tran TS
    The Journal of comparative neurology 409.2 (1999 Jun 28): 285-98.

    Early specification of sensory neuron fate revealed by expression and function of neurogenins in the chick embryo.
    Anderson DJ
    Development (Cambridge, England) 126.8 (1999 Apr): 1715-28.

    Neural induction and regionalisation in the chick embryo.
    Stern CD
    Development (Cambridge, England) 114.3 (1992 Mar): 729-41.

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    A functional study of miR-124 in the developing neural tube.
    Gage FH
    Genes and development 21.5 (2007 Mar 1): 531-6.

    YAP regulates neural progenitor cell number via the TEA domain transcription factor.
    Gage FH
    Genes and development 22.23 (2008 Dec 1): 3320-34.

    Raldh3 gene expression pattern in the developing chicken inner ear.
    Hidalgo-Sánchez M
    The Journal of comparative neurology 514.1 (2009 May 1): 49-65.

    Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification.
    George-Weinstein M
    Developmental biology 359.1 (2011 Nov 1): 12-25.

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