3A10

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$40.00
SKU: 3A10
View product citations for antibody 3A10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neurofilament-associated, Mauthner cells
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Fish, Gecko, Human, Mouse, Planaria, Quail, Rat, Shark, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes a neurofilament-associated antigen. The antibody labels a subset of hindbrain spinal cord projecting neurons such as Mauthner neurons.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chick nervous tissue from ventral spinal cord/cyclophosphamide treatment/assorted nervous tissue
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531874 
Additional Information: 3A10 recognizes a conformational epitope assosciated with the TRSPXP sequence that is shared by neurofilament-associated antigens in the developing rat brain. See PUBMED #10050030.
Recommended Applications: FFPE
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3A10 was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 3A10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

66 References

  • Initial Publication
  • IF References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    Science (New York, N.Y.)
    Jessell TM
    Science (New York, N.Y.) 242.4879 (1988 Nov 4): 692-9.

    IF References

    Developmental neurobiology
    Hatta K
    Developmental neurobiology 74.6 (2014 Jun): 616-32.

    General and comparative endocrinology
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Neuroscience letters
    Placzek M
    Neuroscience letters 562. (2014 Mar 6): 108-13.

    Developmental biology
    Huber AB
    Developmental biology 386.2 (2014 Feb 15): 358-70.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Appel B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.9 (2014 Feb 26): 3402-12.

    Hearing research
    Fekete DM
    Hearing research 310. (2014 Apr): 1-12.

    Developmental neurobiology
    Ali DW
    Developmental neurobiology 75.2 (2015 Feb): 145-62.

    The Journal of biological chemistry
    Rauvala H
    The Journal of biological chemistry 289.29 (2014 Jul 18): 19958-75.

    Disease models & mechanisms
    Briscoe J
    Disease models & mechanisms 7.1 (2014 Jan): 107-17.

    Mechanisms of development
    Dickinson AJ
    Mechanisms of development 133. (2014 Aug): 91-104.

    Neurobiology of disease
    Guthrie S
    Neurobiology of disease 65. (2014 May): 25-34.

    Experimental neurology
    Hu B
    Experimental neurology 251. (2014 Jan): 72-83.

    Neural development
    Ribera AB
    Neural development 9. (2014 Aug 22): 19.

    Developmental biology
    Sagerström CG
    Developmental biology 390.2 (2014 Jun 15): 247-60.

    Nature communications
    Hale ME
    Nature communications 4. (2013): 1729.

    Molecular brain
    Ming GL
    Molecular brain 6. (2013 Dec 1): 51.

    Journal of neuroscience methods
    Tiedemann K
    Journal of neuroscience methods 212.2 (2013 Jan 30): 269-75.

    Stem cell research
    Stern CD
    Stem cell research 10.1 (2013 Jan): 20-8.

    Development (Cambridge, England)
    Jessell TM
    Development (Cambridge, England) 112.1 (1991 May): 51-67.

    PloS one
    Moldovan F
    PloS one 7.2 (2012): e31650.

    Toxicology letters
    Strähle U
    Toxicology letters 314. (2019 Oct 10): 43-52.

    IHC References

    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery
    Wang KC
    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery 30.1 (2014 Jan): 73-82.

    Hearing research
    Fekete DM
    Hearing research 310. (2014 Apr): 1-12.

    Journal of morphology
    Kuratani S
    Journal of morphology 275.1 (2014 Jan): 17-38.

    Disease models & mechanisms
    Gong Y
    Disease models & mechanisms 6.2 (2013 Mar): 404-13.

    Frontiers in neuroanatomy
    Caprile T
    Frontiers in neuroanatomy 8. (2014): 49.

    The Journal of comparative neurology
    Hidalgo-Sánchez M
    The Journal of comparative neurology 521.5 (2013 Apr 1): 1136-64.

    PloS one
    Moldovan F
    PloS one 7.2 (2012): e31650.

    Development (Cambridge, England)
    Lyons DA
    Development (Cambridge, England) 138.20 (2011 Oct): 4443-50.

    Developmental neurobiology
    Chandrasekhar A
    Developmental neurobiology 70.2 (2010 Feb): 87-99.

    Zebrafish
    Peterson RE
    Zebrafish 6.1 (2009 Mar): 79-91.

    Developmental biology
    Chandrasekhar A
    Developmental biology 325.2 (2009 Jan 15): 363-73.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Halloran MC
    Developmental dynamics : an official publication of the American Association of Anatomists 235.1 (2006 Jan): 213-24.

    Proceedings of the National Academy of Sciences of the United States of America
    Hudspeth AJ
    Proceedings of the National Academy of Sciences of the United States of America 101.35 (2004 Aug 31): 13056-61.

    Development (Cambridge, England)
    Okamoto H
    Development (Cambridge, England) 131.15 (2004 Aug): 3705-15.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Beattie CE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.22 (2003 Sep 3): 8159-66.

    Development (Cambridge, England)
    Bally-Cuif L
    Development (Cambridge, England) 129.14 (2002 Jul): 3281-94.

    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
    Huang R
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 205. (2016 May): 85-9.

    Developmental neurobiology
    Drapeau P
    Developmental neurobiology 76.6 (2016 Jun): 642-60.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Hidalgo-Sánchez M
    Developmental dynamics : an official publication of the American Association of Anatomists . (2019 Aug 9): .

    FFPE References

    Orthodontics & craniofacial research
    Golden JA
    Orthodontics & craniofacial research 6.4 (2003 Nov): 210-9.

    All References

    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery
    Wang KC
    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery 30.1 (2014 Jan): 73-82.

    Hearing research
    Fekete DM
    Hearing research 310. (2014 Apr): 1-12.

    Journal of morphology
    Kuratani S
    Journal of morphology 275.1 (2014 Jan): 17-38.

    Disease models & mechanisms
    Gong Y
    Disease models & mechanisms 6.2 (2013 Mar): 404-13.

    Frontiers in neuroanatomy
    Caprile T
    Frontiers in neuroanatomy 8. (2014): 49.

    The Journal of comparative neurology
    Hidalgo-Sánchez M
    The Journal of comparative neurology 521.5 (2013 Apr 1): 1136-64.

    PloS one
    Moldovan F
    PloS one 7.2 (2012): e31650.

    Development (Cambridge, England)
    Lyons DA
    Development (Cambridge, England) 138.20 (2011 Oct): 4443-50.

    Developmental neurobiology
    Chandrasekhar A
    Developmental neurobiology 70.2 (2010 Feb): 87-99.

    Zebrafish
    Peterson RE
    Zebrafish 6.1 (2009 Mar): 79-91.

    Developmental biology
    Chandrasekhar A
    Developmental biology 325.2 (2009 Jan 15): 363-73.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Halloran MC
    Developmental dynamics : an official publication of the American Association of Anatomists 235.1 (2006 Jan): 213-24.

    Proceedings of the National Academy of Sciences of the United States of America
    Hudspeth AJ
    Proceedings of the National Academy of Sciences of the United States of America 101.35 (2004 Aug 31): 13056-61.

    Development (Cambridge, England)
    Okamoto H
    Development (Cambridge, England) 131.15 (2004 Aug): 3705-15.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Beattie CE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.22 (2003 Sep 3): 8159-66.

    Development (Cambridge, England)
    Bally-Cuif L
    Development (Cambridge, England) 129.14 (2002 Jul): 3281-94.

    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
    Huang R
    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 205. (2016 May): 85-9.

    Developmental neurobiology
    Drapeau P
    Developmental neurobiology 76.6 (2016 Jun): 642-60.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Hidalgo-Sánchez M
    Developmental dynamics : an official publication of the American Association of Anatomists . (2019 Aug 9): .

    Developmental neurobiology
    Hatta K
    Developmental neurobiology 74.6 (2014 Jun): 616-32.

    General and comparative endocrinology
    Harvey S
    General and comparative endocrinology 195. (2014 Jan 1): 183-9.

    Neuroscience letters
    Placzek M
    Neuroscience letters 562. (2014 Mar 6): 108-13.

    Developmental biology
    Huber AB
    Developmental biology 386.2 (2014 Feb 15): 358-70.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Appel B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.9 (2014 Feb 26): 3402-12.

    Developmental neurobiology
    Ali DW
    Developmental neurobiology 75.2 (2015 Feb): 145-62.

    The Journal of biological chemistry
    Rauvala H
    The Journal of biological chemistry 289.29 (2014 Jul 18): 19958-75.

    Disease models & mechanisms
    Briscoe J
    Disease models & mechanisms 7.1 (2014 Jan): 107-17.

    Mechanisms of development
    Dickinson AJ
    Mechanisms of development 133. (2014 Aug): 91-104.

    Neurobiology of disease
    Guthrie S
    Neurobiology of disease 65. (2014 May): 25-34.

    Experimental neurology
    Hu B
    Experimental neurology 251. (2014 Jan): 72-83.

    Neural development
    Ribera AB
    Neural development 9. (2014 Aug 22): 19.

    Developmental biology
    Sagerström CG
    Developmental biology 390.2 (2014 Jun 15): 247-60.

    Nature communications
    Hale ME
    Nature communications 4. (2013): 1729.

    Molecular brain
    Ming GL
    Molecular brain 6. (2013 Dec 1): 51.

    Journal of neuroscience methods
    Tiedemann K
    Journal of neuroscience methods 212.2 (2013 Jan 30): 269-75.

    Stem cell research
    Stern CD
    Stem cell research 10.1 (2013 Jan): 20-8.

    Development (Cambridge, England)
    Jessell TM
    Development (Cambridge, England) 112.1 (1991 May): 51-67.

    Toxicology letters
    Strähle U
    Toxicology letters 314. (2019 Oct 10): 43-52.

    Orthodontics & craniofacial research
    Golden JA
    Orthodontics & craniofacial research 6.4 (2003 Nov): 210-9.

    Science (New York, N.Y.)
    Jessell TM
    Science (New York, N.Y.) 242.4879 (1988 Nov 4): 692-9.

    Biotechnology and bioengineering
    Wood MD
    Biotechnology and bioengineering 111.9 (2014 Sep): 1886-94.

    PloS one
    Cole NJ
    PloS one 9.6 (2014): e90572.

    Neural development
    Boeckers TM
    Neural development 8. (2013 Sep 17): 18.

    Development (Cambridge, England)
    McFarlane S
    Development (Cambridge, England) 140.14 (2013 Jul): 2933-41.

    Cellular and molecular life sciences : CMLS
    Shen CJ
    Cellular and molecular life sciences : CMLS 70.5 (2013 Mar): 935-50.

    Genetics and molecular research : GMR
    Jiang HY
    Genetics and molecular research : GMR 13.2 (2014 Jun 9): 4187-201.

    PloS one
    Henley JR
    PloS one 8.3 (2013): e57539.

    Genes to cells : devoted to molecular & cellular mechanisms
    Hirata H
    Genes to cells : devoted to molecular & cellular mechanisms 18.3 (2013 Mar): 211-24.

    Neurobiology of disease
    Allison WT
    Neurobiology of disease 55. (2013 Jul): 11-25.

    Journal of neurophysiology
    Oda Y
    Journal of neurophysiology 111.6 (2014 Mar): 1153-64.

    BMC developmental biology
    Sagerström CG
    BMC developmental biology 14. (2014 Jun 5): 25.

    Development (Cambridge, England)
    Sela-Donenfeld D
    Development (Cambridge, England) 140.10 (2013 May): 2190-202.

    Neuroscience
    Huang R
    Neuroscience 246. (2013 Aug 29): 22-7.

    PloS one
    Granato M
    PloS one 8.1 (2013): e54071.

    Neuroscience letters
    Xu H
    Neuroscience letters 553. (2013 Oct 11): 104-9.

    Development (Cambridge, England)
    Placzek M
    Development (Cambridge, England) 140.5 (2013 Mar): 1111-22.

    Cell
    Tessier-Lavigne M
    Cell 87.6 (1996 Dec 13): 1001-14.

    Cell and tissue research
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    The Journal of comparative neurology
    Tran TS
    The Journal of comparative neurology 409.2 (1999 Jun 28): 285-98.

    Development (Cambridge, England)
    Anderson DJ
    Development (Cambridge, England) 126.8 (1999 Apr): 1715-28.

    Development (Cambridge, England)
    Stern CD
    Development (Cambridge, England) 114.3 (1992 Mar): 729-41.

    Genesis (New York, N.Y. : 2000)
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Genes & development
    Gage FH
    Genes & development 21.5 (2007 Mar 1): 531-6.

    Genes & development
    Gage FH
    Genes & development 22.23 (2008 Dec 1): 3320-34.

    The Journal of comparative neurology
    Hidalgo-Sánchez M
    The Journal of comparative neurology 514.1 (2009 May 1): 49-65.

    Developmental biology
    George-Weinstein M
    Developmental biology 359.1 (2011 Nov 1): 12-25.

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