2G3-3D7
Catalog Fields
Antigen: alpha-actinin, sarcomeric
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is also known as alpha:1.
Antigen Molecular Weight: 107.0 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Actn
Immunogen: Purified Z-discs
Alternate Gene Names: fliA, l(1)2Cb, Dmel_CG4376
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P18091
Immunogen Sequence:
Entrez Gene ID: 31166
Additional Characterization:
Antibody Registry ID: AB_2721943
Additional Information: FBgn0000667 RRID:AB_2721943
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2G3-3D7 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 2G3-3D7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.
Pardue ML
The Journal of cell biology 109.5 (1989 Nov): 2157-67.