Catalog Fields
Antigen: alpha-actinin, sarcomeric
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Saide, J.D.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Boston University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody is also known as alpha:1.
Predicted Species Reactivity:
Gene: Actn
Immunogen: Purified Z-discs
Alternate Gene Names: fliA, l(1)2Cb, Dmel_CG4376
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P18091
Immunogen Sequence:
Entrez Gene ID: 31166
Antibody Registry ID: AB_2721943
Additional Information: FBgn0000667 RRID:AB_2721943
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2G3-3D7 was deposited to the DSHB by Saide, J.D. (DSHB Hybridoma Product 2G3-3D7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.
Pardue ML
The Journal of cell biology 109.5 (1989 Nov): 2157-67.