3A9 (323 or M10-2)

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$40.00
SKU: 3A9 (323 or M10-2)
View product citations for antibody 3A9 (323 or M10-2) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Spectrin, alpha
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Branton, D. / Dubreuil, R.
Isotype: MIgG1
Depositors Institution: Biology, Harvard University
Host Species: mouse
Gene: alpha-Spec
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: SPEC-A; alpha-spectrin; alpha-Spectrin; alphaSp; alphaSpec; alphaSpectrin; Spectrin
Antigen Molecular Weight: Predicted: 278kDa; Apparent: 234kDa
Clonality: Monoclonal
Immunogen: Drosophila alpha spectrin-Trp E fusion protein produced in Escherichia coli
Immunogen Sequence: Recombinant alpha spectrin, Partial protein
Myeloma Strain: Fox NY
Uniprot ID: P13395 
Entrez Gene ID: 38231 
Antibody Registry ID: AB_528473 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3A9 (323 or M10-2) was deposited to the DSHB by Branton, D. / Dubreuil, R. (DSHB Hybridoma Product 3A9 (323 or M10-2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

55 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Drosophilia spectrin. I. Characterization of the purified protein.
    Kiehart DP
    The Journal of cell biology 105.5 (1987 Nov): 2095-102.

    IF References

    Segregation of two spectrin isoforms: polarized membrane-binding sites direct polarized membrane skeleton assembly.
    MacVicar GR
    Molecular biology of the cell 8.10 (1997 Oct): 1933-42.

    The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline.
    Horabin JI
    Development (Cambridge, England) 128.14 (2001 Jul): 2649-60.

    RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
    Lehmann R
    Nature cell biology 12.6 (2010 Jun): 605-10.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    The Arf family G protein Arl1 is required for secretory granule biogenesis in Drosophila.
    Munro S
    Journal of cell science 127.Pt 10 (2014 May 15): 2151-60.

    Specification of regional intestinal stem cell identity during Drosophila metamorphosis.
    Ohlstein B
    Development (Cambridge, England) 141.9 (2014 May): 1848-56.

    The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila.
    Luschnig S
    Development (Cambridge, England) 141.4 (2014 Feb): 899-908.

    A dual role for integrin-linked kinase and β1-integrin in modulating cardiac aging.
    Ocorr K
    Aging cell 13.3 (2014 Jun): 431-40.

    The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila.
    Geyer PK
    Genetics 197.2 (2014 Jun): 653-65.

    The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium.
    Rabouille C
    Nucleic acids research 42.3 (2014 Feb): 1970-86.

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Muscle dystroglycan organizes the postsynapse and regulates presynaptic neurotransmitter release at the Drosophila neuromuscular junction.
    Parmentier ML
    PloS one 3.4 (2008 Apr 30): e2084.

    The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    Li M
    Developmental biology 384.1 (2013 Dec 1): 1-12.

    Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology].
    Thompson BJ
    BMC developmental biology 9. (2009 Feb 19): 14.

    Retromer regulates apical-basal polarity through recycling Crumbs.
    Lin X
    Developmental biology 360.1 (2011 Dec 1): 87-95.

    Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system.
    Knoblich JA
    Cell 107.2 (2001 Oct 19): 183-94.

    A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
    St Johnston D
    Nature 421.6921 (2003 Jan 23): 379-84.

    Spatially defined Dsh-Lgl interaction contributes to directional tissue morphogenesis.
    Tolwinski NS
    Journal of cell science 123.Pt 18 (2010 Sep 15): 3157-65.

    Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin.
    Budnik V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.5 (2007 Jan 31): 1033-44.

    Monomeric α-catenin links cadherin to the actin cytoskeleton.
    Tepass U
    Nature cell biology 15.3 (2013 Mar): 261-73.

    The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis.
    Geyer PK
    Developmental biology 356.2 (2011 Aug 15): 398-410.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    asunder is required for dynein localization and dorsal fate determination during Drosophila oogenesis.
    Lee LA
    Developmental biology 386.1 (2014 Feb 1): 42-52.

    Evidence for chromatin-remodeling complex PBAP-controlled maintenance of the Drosophila ovarian germline stem cells.
    Li M
    PloS one 9.7 (2014): e103473.

    Centralspindlin is required for thorax development during Drosophila metamorphosis.
    Sfregola M
    Genesis (New York, N.Y. : 2000) 52.5 (2014 May): 387-98.

    The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma.
    Papagiannouli F
    The International journal of developmental biology 57.1 (2013): 25-34.

    A temporal signature of epidermal growth factor signaling regulates the differentiation of germline cells in testes of Drosophila melanogaster.
    Schulz C
    PloS one 8.8 (2013): e70678.

    dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary.
    Li M
    Developmental biology 379.2 (2013 Jul 15): 167-81.

    A functional role of the extracellular domain of Crumbs in cell architecture and apicobasal polarity.
    Llimargas M
    Journal of cell science 126.Pt 10 (2013 May 15): 2157-63.

    Cellular mechanics of germ band retraction in Drosophila.
    Hutson MS
    Developmental biology 384.2 (2013 Dec 15): 205-13.

    Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup.
    Fortini ME
    Development (Cambridge, England) 140.14 (2013 Jul): 3018-27.

    Innexin 3, a new gene required for dorsal closure in Drosophila embryo.
    Rabouille C
    PloS one 8.7 (2013): e69212.

    Akt regulates glutamate receptor trafficking and postsynaptic membrane elaboration at the Drosophila neuromuscular junction.
    Selleck SB
    Developmental neurobiology 73.10 (2013 Oct): 723-43.

    The bHLH transcription factor hand is required for proper wing heart formation in Drosophila.
    Paululat A
    Developmental biology 381.2 (2013 Sep 15): 446-59.

    The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.
    Wang Z
    Developmental biology 376.2 (2013 Apr 15): 163-70.

    Following the 'tracks': Tramtrack69 regulates epithelial tube expansion in the Drosophila ovary through Paxillin, Dynamin, and the homeobox protein Mirror.
    Berg CA
    Developmental biology 378.2 (2013 Jun 15): 154-69.

    Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development.
    Wu CT
    PLoS genetics 9.12 (2013): e1004013.

    Multicolor fluorescence imaging of whole-mount Drosophila testes for studying spermatogenesis.
    Olenina LV
    Analytical biochemistry 436.1 (2013 May 1): 55-64.

    The Drosophila nuclear lamina protein otefin is required for germline stem cell survival.
    Geyer PK
    Developmental cell 25.6 (2013 Jun 24): 645-54.

    The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells.
    Thompson BJ
    The Journal of cell biology 201.6 (2013 Jun 10): 875-85.

    Germ cell connectivity enhances cell death in response to DNA damage in the <i>Drosophila</i> testis.
    Yamashita YM
    eLife 6. (2017 Aug 15): .

    α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton.
    Feany MB
    Neuron 97.1 (2018 Jan 3): 108-124.e6.

    Distinct domains in the matricellular protein Lonely heart are crucial for cardiac extracellular matrix formation and heart function in <i>Drosophila</i>.
    Paululat A
    The Journal of biological chemistry 293.20 (2018 May 18): 7864-7879.

    <i>rebuff</i> regulates apical luminal matrix to control tube size in <i>Drosophila</i> trachea.
    Jiang L
    Biology open 7.9 (2018 Sep 5): .

    WB References
    IP References
    All References

    Segregation of two spectrin isoforms: polarized membrane-binding sites direct polarized membrane skeleton assembly.
    MacVicar GR
    Molecular biology of the cell 8.10 (1997 Oct): 1933-42.

    The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline.
    Horabin JI
    Development (Cambridge, England) 128.14 (2001 Jul): 2649-60.

    RhoL controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
    Lehmann R
    Nature cell biology 12.6 (2010 Jun): 605-10.

    The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster.
    Paululat A
    Developmental biology 350.1 (2011 Feb 1): 89-100.

    The Arf family G protein Arl1 is required for secretory granule biogenesis in Drosophila.
    Munro S
    Journal of cell science 127.Pt 10 (2014 May 15): 2151-60.

    Specification of regional intestinal stem cell identity during Drosophila metamorphosis.
    Ohlstein B
    Development (Cambridge, England) 141.9 (2014 May): 1848-56.

    The transmembrane protein Macroglobulin complement-related is essential for septate junction formation and epithelial barrier function in Drosophila.
    Luschnig S
    Development (Cambridge, England) 141.4 (2014 Feb): 899-908.

    A dual role for integrin-linked kinase and β1-integrin in modulating cardiac aging.
    Ocorr K
    Aging cell 13.3 (2014 Jun): 431-40.

    The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells.
    Fuller MT
    Development (Cambridge, England) 141.1 (2014 Jan): 73-82.

    Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila.
    Geyer PK
    Genetics 197.2 (2014 Jun): 653-65.

    The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium.
    Rabouille C
    Nucleic acids research 42.3 (2014 Feb): 1970-86.

    miR-8 controls synapse structure by repression of the actin regulator enabled.
    Van Vactor D
    Development (Cambridge, England) 141.9 (2014 May): 1864-74.

    Muscle dystroglycan organizes the postsynapse and regulates presynaptic neurotransmitter release at the Drosophila neuromuscular junction.
    Parmentier ML
    PloS one 3.4 (2008 Apr 30): e2084.

    The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
    Li M
    Developmental biology 384.1 (2013 Dec 1): 1-12.

    Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology].
    Thompson BJ
    BMC developmental biology 9. (2009 Feb 19): 14.

    Retromer regulates apical-basal polarity through recycling Crumbs.
    Lin X
    Developmental biology 360.1 (2011 Dec 1): 87-95.

    Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous system.
    Knoblich JA
    Cell 107.2 (2001 Oct 19): 183-94.

    A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
    St Johnston D
    Nature 421.6921 (2003 Jan 23): 379-84.

    Spatially defined Dsh-Lgl interaction contributes to directional tissue morphogenesis.
    Tolwinski NS
    Journal of cell science 123.Pt 18 (2010 Sep 15): 3157-65.

    Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin.
    Budnik V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.5 (2007 Jan 31): 1033-44.

    Monomeric α-catenin links cadherin to the actin cytoskeleton.
    Tepass U
    Nature cell biology 15.3 (2013 Mar): 261-73.

    The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis.
    Geyer PK
    Developmental biology 356.2 (2011 Aug 15): 398-410.

    A regulatory network of Drosophila germline stem cell self-renewal.
    Perrimon N
    Developmental cell 28.4 (2014 Feb 24): 459-73.

    The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity.
    Paululat A
    PLoS genetics 9.7 (2013): e1003616.

    asunder is required for dynein localization and dorsal fate determination during Drosophila oogenesis.
    Lee LA
    Developmental biology 386.1 (2014 Feb 1): 42-52.

    Evidence for chromatin-remodeling complex PBAP-controlled maintenance of the Drosophila ovarian germline stem cells.
    Li M
    PloS one 9.7 (2014): e103473.

    Centralspindlin is required for thorax development during Drosophila metamorphosis.
    Sfregola M
    Genesis (New York, N.Y. : 2000) 52.5 (2014 May): 387-98.

    The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma.
    Papagiannouli F
    The International journal of developmental biology 57.1 (2013): 25-34.

    A temporal signature of epidermal growth factor signaling regulates the differentiation of germline cells in testes of Drosophila melanogaster.
    Schulz C
    PloS one 8.8 (2013): e70678.

    dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary.
    Li M
    Developmental biology 379.2 (2013 Jul 15): 167-81.

    A functional role of the extracellular domain of Crumbs in cell architecture and apicobasal polarity.
    Llimargas M
    Journal of cell science 126.Pt 10 (2013 May 15): 2157-63.

    Cellular mechanics of germ band retraction in Drosophila.
    Hutson MS
    Developmental biology 384.2 (2013 Dec 15): 205-13.

    Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup.
    Fortini ME
    Development (Cambridge, England) 140.14 (2013 Jul): 3018-27.

    Innexin 3, a new gene required for dorsal closure in Drosophila embryo.
    Rabouille C
    PloS one 8.7 (2013): e69212.

    Akt regulates glutamate receptor trafficking and postsynaptic membrane elaboration at the Drosophila neuromuscular junction.
    Selleck SB
    Developmental neurobiology 73.10 (2013 Oct): 723-43.

    The bHLH transcription factor hand is required for proper wing heart formation in Drosophila.
    Paululat A
    Developmental biology 381.2 (2013 Sep 15): 446-59.

    The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.
    Wang Z
    Developmental biology 376.2 (2013 Apr 15): 163-70.

    Following the 'tracks': Tramtrack69 regulates epithelial tube expansion in the Drosophila ovary through Paxillin, Dynamin, and the homeobox protein Mirror.
    Berg CA
    Developmental biology 378.2 (2013 Jun 15): 154-69.

    Germline progenitors escape the widespread phenomenon of homolog pairing during Drosophila development.
    Wu CT
    PLoS genetics 9.12 (2013): e1004013.

    Multicolor fluorescence imaging of whole-mount Drosophila testes for studying spermatogenesis.
    Olenina LV
    Analytical biochemistry 436.1 (2013 May 1): 55-64.

    The Drosophila nuclear lamina protein otefin is required for germline stem cell survival.
    Geyer PK
    Developmental cell 25.6 (2013 Jun 24): 645-54.

    The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells.
    Thompson BJ
    The Journal of cell biology 201.6 (2013 Jun 10): 875-85.

    Germ cell connectivity enhances cell death in response to DNA damage in the <i>Drosophila</i> testis.
    Yamashita YM
    eLife 6. (2017 Aug 15): .

    α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton.
    Feany MB
    Neuron 97.1 (2018 Jan 3): 108-124.e6.

    Distinct domains in the matricellular protein Lonely heart are crucial for cardiac extracellular matrix formation and heart function in <i>Drosophila</i>.
    Paululat A
    The Journal of biological chemistry 293.20 (2018 May 18): 7864-7879.

    <i>rebuff</i> regulates apical luminal matrix to control tube size in <i>Drosophila</i> trachea.
    Jiang L
    Biology open 7.9 (2018 Sep 5): .

    Drosophilia spectrin. I. Characterization of the purified protein.
    Kiehart DP
    The Journal of cell biology 105.5 (1987 Nov): 2095-102.

    A genetic RNAi screen for IP₃/Ca²⁺ coupled GPCRs in Drosophila identifies the PdfR as a regulator of insect flight.
    Hasan G
    PLoS genetics 9.10 (2013): e1003849.

    A beta-spectrin isoform from Drosophila (beta H) is similar in size to vertebrate dystrophin.
    Kiehart DP
    The Journal of cell biology 111.5 Pt 1 (1990 Nov): 1849-58.

    Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila.
    Hays TS
    The Journal of cell biology 189.1 (2010 Apr 5): 143-58.

    Drosophila spectrin: the membrane skeleton during embryogenesis.
    Branton D
    The Journal of cell biology 108.5 (1989 May): 1697-709.

    Functional antagonism between histone H3K4 demethylases in vivo.
    Dyson NJ
    Genes & development 25.1 (2011 Jan 1): 17-28.

    UNC79 and UNC80, putative auxiliary subunits of the NARROW ABDOMEN ion channel, are indispensable for robust circadian locomotor rhythms in Drosophila.
    Allada R
    PloS one 8.11 (2013): e78147.

    AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction.
    Serpe M
    Genes & development 26.9 (2012 May 1): 974-87.

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