Antigen: Spectrin, alpha
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Branton, D. / Dubreuil, R.
Host Species: mouse
Depositors Institution: Biology, Harvard University
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Predicted: 278kDa; Apparent: 234kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Drosophila alpha spectrin-Trp E fusion protein produced in Escherichia coli
Alternate Gene Names: SPEC-A; alpha-spectrin; alpha-Spectrin; alphaSp; alphaSpec; alphaSpectrin; Spectrin
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Fox NY
Epitope Location or Sequence:
Uniprot ID: P13395
Immunogen Sequence: Recombinant alpha spectrin, Partial protein
Entrez Gene ID: 38231
Antibody Registry ID: AB_528473
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3A9 (323 or M10-2) was deposited to the DSHB by Branton, D. / Dubreuil, R. (DSHB Hybridoma Product 3A9 (323 or M10-2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).