7G10 anti-Fasciclin III
Catalog Fields
Clone ID/Product Name: 7G10 anti-Fasciclin III
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Fas3
Ab Isotype: MIgG2a
Gene Name:
Entrez Gene ID: 35097
Clonality: Monoclonal
Immunogen: Membrane preparation from nerve cord and cultures of primary neurons (10-13 hr development).
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Fasciclin III (Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/1/1994
Antigen Molecular Weight: Multiple isoforms; Apparent: 46, 59, 66, and 80 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Drosophila
Depositor Notes: Fas III is expressed on a subset of neurons and axons in the CNS, patches of ectoderm, ectodermal stripes, parts of the hindgut and foregut, visceral mesoderm, and the salivary gland. The antibody can be used to stain dissections without detergent as the epitope is on the extracellular domain of the protein. After 10 hrs in development, ectodermal staining will obscure CNS in whole mount preps and therefore dissected preparations are recommended for embryos staining past this age. During larval development, staining is present on a subset of neurons, the epithelial layer of the discs and a cyclone fence pattern in the eye disc. It is especially useful for following the development of the RP1 and RP3 neurons. The antibody works for affinity purification but does not work for immunoprecipitation or western.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This antibody may work for Immunoprecipitation. While the Depositor clearly stated that it doesn't work for immunoprecipitation (see Depositor Notes), the Initial Publication, in which the Depositor is the last author, reports the succesful use of 7G10 for the immunoprecipitation of Fasciclin III.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation