3H5

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$40.00
SKU: 3H5
View product citations for antibody 3H5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: HNK-1 carbohydrate epitope
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Neurobiology, University of Pittsburgh
Positive Tested Species Reactivity: Chicken, Human, Lamprey, Shark
Depositors Notes: The HNK-1 epitope is a N-linked carbohydrate marker of neural crest cells and of a subset of lymphocytes. This marker is also known as CD57.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chicken optic nerve (E10)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence: SO4-3GlcAb1-3Galb1-4GlcNAc
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2314644 
Additional Information: 3H5 is a mouse hybridoma that was generated against chick optic nerve, and is not a subclone of the VC1.1 hybridoma which is a mouse IgM and also recognizes the HNK-1 epitope.
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3H5 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 3H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum.
    De Bellard ME
    The Journal of comparative neurology 521.14 (2013 Oct 1): 3303-20.

    IF References

    Neural crest stem cells and their potential therapeutic applications.
    Cheung M
    Developmental biology 419.2 (2016 Nov 15): 199-216.

    Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum.
    De Bellard ME
    The Journal of comparative neurology 521.14 (2013 Oct 1): 3303-20.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    A fate-map for cranial sensory ganglia in the sea lamprey.
    Baker CV
    Developmental biology 385.2 (2014 Jan 15): 405-16.

    IHC References
    All References

    Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube.
    Bronner ME
    Molecular biology of the cell 25.3 (2014 Feb): 347-55.

    Neural crest stem cells and their potential therapeutic applications.
    Cheung M
    Developmental biology 419.2 (2016 Nov 15): 199-216.

    Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum.
    De Bellard ME
    The Journal of comparative neurology 521.14 (2013 Oct 1): 3303-20.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    A fate-map for cranial sensory ganglia in the sea lamprey.
    Baker CV
    Developmental biology 385.2 (2014 Jan 15): 405-16.

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