1C10

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$40.00
SKU: 1C10
View product citations for antibody 1C10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: HNK-1 epitope
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Halfter, W.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Neurobiology, University of Pittsburgh
Positive Tested Species Reactivity: Chicken, Human
Depositors Notes: The chemical properties of the antigen are HNK-1 epitope with carbohydrate characterization.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chicken optic nerve
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation:X63Ag8.653
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_10570406 
Additional Information: The developmental stage of the Immunogen is E10. After immunization route IP 100 micrograms of adjuvant 1st injection Freund's complete, following injection Freund's incomplete was used. RRID:AB_10570406
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1C10 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 1C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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