EMA-1
Catalog Fields
Antigen: primordial germ cell surface marker (mouse)
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgM
Depositor: Eddy, M. / Hahnel, A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Amphibian, Avian, Broad species, Fish, Mammal, Zebrafish
Depositors Institution: NIH, NIEHS, RTP and Ontario Veterinary College
Antigen Molecular Weight: Apparent: +500 kDa
Depositors Notes: This antibody binds specifically to the surface of primordial germ cells and a variety of adult apical epithelium including oviduct and proximal kidney. This antibody also recognizes antigen in olfactory pits and membranous portions of fins in zebrafish PMID: 19302835.
Immunogen: Nulli SCC1 EC cells (fixed in 1% formaldehyde + 1% glutaraldehyde)
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1
Epitope Mapped: Yes
Epitope Location or Sequence: EMA-1 recognizes short chain (monofucosylated polylactosamine) and long chain (mono- and difucosylated polylactosamine) glycolipids of cell surface glycoproteins specific of primordial germ cells.
Antibody Registry ID: AB_531885
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: This antibody was originally described and characterized in [Hahnel, A.C., and Eddy, E.M. (1986). Cell surface markers of mouse primordial germ cells defined by two monoclonal antibodies. Gamete Research 15, 25-34]. RRID:AB_531885
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EMA-1 was deposited to the DSHB by Eddy, M. / Hahnel, A. (DSHB Hybridoma Product EMA-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
10 References
Initial Publication
The distribution of two cell surface determinants of mouse embryonal carcinoma and early embryonic cells.
Eddy EM
Journal of reproductive immunology 10.2 (1987 Feb): 89-110.