EMA-1

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SKU: EMA-1-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: EMA-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531885 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Nulli SCC1 EC cells (fixed in 1% formaldehyde + 1% glutaraldehyde)
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: Yes
Antigen Name: primordial germ cell surface marker (mouse)
Epitope Location or Sequence: EMA-1 recognizes short chain (monofucosylated polylactosamine) and long chain (mono- and difucosylated polylactosamine) glycolipids of cell surface glycoproteins specific of primordial germ cells.
Alternate Antigen Name:
Deposit Date: 9/7/1994
Antigen Molecular Weight: Apparent: +500 kDa
Depositor: Eddy, M. / Hahnel, A.
Antigen Sequence:
Depositor Institution: NIH, NIEHS, RTP and Ontario Veterinary College
Antigen Species: Mouse
Depositor Notes: This antibody binds specifically to the surface of primordial germ cells and a variety of adult apical epithelium including oviduct and proximal kidney. This antibody also recognizes antigen in olfactory pits and membranous portions of fins in zebrafish PMID: 19302835.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Amphibian, Avian, Broad species, Fish, Mammal, Zebrafish
Additional Information: This antibody was originally described and characterized in [Hahnel, A.C., and Eddy, E.M. (1986). Cell surface markers of mouse primordial germ cells defined by two monoclonal antibodies. Gamete Research 15, 25-34]. RRID:AB_531885
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EMA-1 was deposited to the DSHB by Eddy, M. / Hahnel, A. (DSHB Hybridoma Product EMA-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Reprogramming of primordial germ cells begins before migration into the genital ridge, making these cells inadequate donors for reproductive cloning.
    Bartolomei MS
    Proceedings of the National Academy of Sciences of the United States of America 100.21 (2003 Oct 14): 12207-12.

    Isolation of viable mouse primordial germ cells by antibody-directed flow sorting.
    Bolen JL
    The Journal of experimental zoology 242.1 (1987 Apr): 107-11.

    In vitro generation and characterization of chicken long-term germ cells from different embryonic origins.
    Pain B
    Theriogenology 84.5 (2015 Sep 15): 732-42.e1-2.

    Rapid adaptation of molecular resources from zebrafish and medaka to develop an estuarine/marine model.
    Cheng SH
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 149.4 (2009 May): 647-55.

    Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis.
    Hua J
    Journal of cellular biochemistry 112.4 (2011 Apr): 1009-21.

    Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
    Etches RJ
    Development (Cambridge, England) 122.8 (1996 Aug): 2339-48.

    WB References
    IHC References
    FFPE References

    The distribution of two cell surface determinants of mouse embryonal carcinoma and early embryonic cells.
    Eddy EM
    Journal of reproductive immunology 10.2 (1987 Feb): 89-110.

    Reprogramming of primordial germ cells begins before migration into the genital ridge, making these cells inadequate donors for reproductive cloning.
    Bartolomei MS
    Proceedings of the National Academy of Sciences of the United States of America 100.21 (2003 Oct 14): 12207-12.

    FACS References

    Isolation of viable mouse primordial germ cells by antibody-directed flow sorting.
    Bolen JL
    The Journal of experimental zoology 242.1 (1987 Apr): 107-11.

    Epitope Map References
    All References

    The distribution of two cell surface determinants of mouse embryonal carcinoma and early embryonic cells.
    Eddy EM
    Journal of reproductive immunology 10.2 (1987 Feb): 89-110.

    Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells.
    Hanley NA
    Stem cells (Dayton, Ohio) 21.5 (2003): 598-609.

    Characterization of mesenchymal stem cells (MSCs) from human fetal lung: potential differentiation of germ cells.
    Dou Z
    Tissue & cell 41.6 (2009 Dec): 448-55.

    Isolation of chicken primordial germ cells using fluorescence-activated cell sorting.
    Petitte JN
    Poultry science 84.4 (2005 Apr): 594-600.

    Reprogramming of primordial germ cells begins before migration into the genital ridge, making these cells inadequate donors for reproductive cloning.
    Bartolomei MS
    Proceedings of the National Academy of Sciences of the United States of America 100.21 (2003 Oct 14): 12207-12.

    Isolation of viable mouse primordial germ cells by antibody-directed flow sorting.
    Bolen JL
    The Journal of experimental zoology 242.1 (1987 Apr): 107-11.

    In vitro generation and characterization of chicken long-term germ cells from different embryonic origins.
    Pain B
    Theriogenology 84.5 (2015 Sep 15): 732-42.e1-2.

    Rapid adaptation of molecular resources from zebrafish and medaka to develop an estuarine/marine model.
    Cheng SH
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 149.4 (2009 May): 647-55.

    Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis.
    Hua J
    Journal of cellular biochemistry 112.4 (2011 Apr): 1009-21.

    Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
    Etches RJ
    Development (Cambridge, England) 122.8 (1996 Aug): 2339-48.

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