MC-480 (SSEA-1)

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Product Name/ID: MC-480 (SSEA-1)
Available to For-Profits: Yes
Alternate Antibody Name: anti-CD15, anti-Lewis X
Gene Name: Fut4
Ab Isotype: MIgM, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528475 
Uniprot ID: Q11127 
RRID:  
Entrez Gene ID: 14345 
Clonality: Monoclonal
Immunogen: Irradiated testis embryonal carcinoma F9 cell line
Clone:
Immunogen Sequence:
Myeloma Strain: P3-X63-Ag8
Epitope Mapped: Yes
Antigen Name: Stage-specific embryonic antigen 1/CD15
Epitope Location or Sequence: SSEA-1 is a carbohydrate epitope on glycolipids and glycoproteins involving fucosylated [GalBeta1-4(FucAlpha1-3)GlcNAcBeta1-R] type 2 blood group chains.
Alternate Antigen Name:
Deposit Date: 6/11/1987
Antigen Molecular Weight:
Depositor: Solter, D. / Knowles, B.B
Antigen Sequence:
Depositor Institution: Wistar Institute
Antigen Species: Mouse
Depositor Notes: MC-480 recognizes mouse embryos, mouse embryonic cells (EC), mouse embryonic stem cells (ES) and mouse & human embryonic germ cells (EG). MC-480 does not bind to human EC, ES or iPS cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bovine, Chicken, Drosophila, Equine, Human, Mouse, Primate, Rat
Additional Information: This antigen has been identified as Alpha-(1,3)-fucosyltransferase 4.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MC-480 (SSEA-1) was deposited to the DSHB by Solter, D. / Knowles, B.B (DSHB Hybridoma Product MC-480 (SSEA-1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

47 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FACS References
  • Epitope Map References
  • All References
    • Initial Publication

    Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
    Knowles BB
    Proceedings of the National Academy of Sciences of the United States of America 75.11 (1978 Nov): 5565-9.

    IF References

    Differential regulation of proliferation and neuronal differentiation in adult rat spinal cord neural stem/progenitors by ERK1/2, Akt, and PLCγ.
    Recio-Pinto E
    Frontiers in molecular neuroscience 6. (2013): 23.

    Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions.
    Longmore GD
    Molecular biology of the cell 11.10 (2000 Oct): 3299-313.

    c-Jun amino-terminal kinase is regulated by Galpha12/Galpha13 and obligate for differentiation of P19 embryonal carcinoma cells by retinoic acid.
    Malbon CC
    The Journal of biological chemistry 272.39 (1997 Sep 26): 24468-74.

    Formin proteins of the DAAM subfamily play a role during axon growth.
    Mihály J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.49 (2008 Dec 3): 13310-9.

    Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
    Ramalho-Santos M
    Nature 460.7257 (2009 Aug 13): 863-8.

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid.
    Strickland S
    Developmental biology 70.2 (1979 Jun): 515-21.

    Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm.
    Solter D
    Experimental cell research 137.2 (1982 Feb): 485-8.

    Distribution of murine stage-specific embryonic antigens in the kidneys of three rodent species.
    Dawjanov I
    Experimental cell research 140.2 (1982 Aug): 331-9.

    Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.
    Fraley EE
    The American journal of pathology 108.2 (1982 Aug): 225-30.

    Monoclonal antibody against SSEA-1 is specific for a subpopulation of astrocytes in mouse cerebellum.
    Knowles B
    Neuroscience letters 31.2 (1982 Aug 16): 181-4.

    Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.
    Solter D
    Cancer research 43.2 (1983 Feb): 669-78.

    Episome-generated N-myc antisense RNA restricts the differentiation potential of primitive neuroectodermal cell lines.
    Neckers LM
    Molecular and cellular biology 11.3 (1991 Mar): 1360-71.

    Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
    Etches RJ
    Development (Cambridge, England) 122.8 (1996 Aug): 2339-48.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines.
    Smith AG
    Biology of reproduction 68.1 (2003 Jan): 222-9.

    Combined effects of Matrigel and growth factors on maintaining undifferentiated murine embryonic stem cells for embryotoxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 18.4 (2004 Aug): 543-53.

    Failure to express GAP-43 leads to disruption of a multipotent precursor and inhibits astrocyte differentiation.
    Meiri KF
    Molecular and cellular neurosciences 26.3 (2004 Jul): 390-405.

    Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 19.3 (2005 Apr): 389-97.

    Periderm prevents pathological epithelial adhesions during embryogenesis.
    Dixon MJ
    The Journal of clinical investigation 124.9 (2014 Sep): 3891-900.

    Efficient long-term cryopreservation of pluripotent stem cells at -80 °C.
    Han X
    Scientific reports 6. (2016 Oct 3): 34476.

    Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer's Disease.
    Soto C
    Cells 10.7 (2021 Jul 16): .

    WB References

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    IHC References

    Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
    Imai H
    Genes to cells : devoted to molecular & cellular mechanisms 16.7 (2011 Jul): 815-25.

    Rostro-caudal gradual loss of cellular diversity within the periventricular regions of the ventricular system.
    Storch A
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 928-41.

    Derivation of pluripotent stem cells from cultured human primordial germ cells.
    Gearhart JD
    Proceedings of the National Academy of Sciences of the United States of America 95.23 (1998 Nov 10): 13726-31.

    Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues.
    Solter D
    Developmental biology 83.2 (1981 Apr 30): 391-8.

    Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.
    Fraley EE
    The American journal of pathology 108.2 (1982 Aug): 225-30.

    Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.
    Solter D
    Cancer research 43.2 (1983 Feb): 669-78.

    Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain.
    Spicer SS
    Glycoconjugate journal 13.3 (1996 Jun): 367-75.

    Nodal and bone morphogenetic protein 5 interact in murine mesoderm formation and implantation.
    Iannaccone PM
    Genesis (New York, N.Y. : 2000) 28.1 (2000 Sep): 1-14.

    The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.
    Calame KL
    Mechanisms of development 117.1-2 (2002 Sep): 305-9.

    Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells.
    Hanley NA
    Stem cells (Dayton, Ohio) 21.5 (2003): 598-609.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    IP References

    SSEA-1, a stage-specific embryonic antigen of the mouse, is carried by the glycoprotein-bound large carbohydrate in embryonal carcinoma cells.
    Solter D
    Cell differentiation 16.3 (1985 May): 169-73.

    FFPE References

    Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
    Ramalho-Santos M
    Nature 460.7257 (2009 Aug 13): 863-8.

    Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers.
    Wong CH
    Proceedings of the National Academy of Sciences of the United States of America 111.7 (2014 Feb 18): 2482-7.

    FACS References

    Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
    Rao MS
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 243-58.

    Long-term culture of human embryonic stem cells in feeder-free conditions.
    Carpenter MK
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 259-74.

    A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells.
    Jin J
    Nature chemical biology 7.8 (2011 Jul 10): 566-74.

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    Pluripotency of mesenchymal stem cells derived from adult marrow.
    Verfaillie CM
    Nature 418.6893 (2002 Jul 4): 41-9.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Epitope Map References

    Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains.
    Evans MJ
    Nature 292.5819 (1981 Jul 9): 156-8.

    The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note.
    Knowles BB
    Biochemical and biophysical research communications 100.4 (1981 Jun): 1578-86.

    All References

    Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
    Imai H
    Genes to cells : devoted to molecular & cellular mechanisms 16.7 (2011 Jul): 815-25.

    Rostro-caudal gradual loss of cellular diversity within the periventricular regions of the ventricular system.
    Storch A
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 928-41.

    Derivation of pluripotent stem cells from cultured human primordial germ cells.
    Gearhart JD
    Proceedings of the National Academy of Sciences of the United States of America 95.23 (1998 Nov 10): 13726-31.

    Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues.
    Solter D
    Developmental biology 83.2 (1981 Apr 30): 391-8.

    Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.
    Fraley EE
    The American journal of pathology 108.2 (1982 Aug): 225-30.

    Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.
    Solter D
    Cancer research 43.2 (1983 Feb): 669-78.

    Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain.
    Spicer SS
    Glycoconjugate journal 13.3 (1996 Jun): 367-75.

    Nodal and bone morphogenetic protein 5 interact in murine mesoderm formation and implantation.
    Iannaccone PM
    Genesis (New York, N.Y. : 2000) 28.1 (2000 Sep): 1-14.

    The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.
    Calame KL
    Mechanisms of development 117.1-2 (2002 Sep): 305-9.

    Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells.
    Hanley NA
    Stem cells (Dayton, Ohio) 21.5 (2003): 598-609.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Differential regulation of proliferation and neuronal differentiation in adult rat spinal cord neural stem/progenitors by ERK1/2, Akt, and PLCγ.
    Recio-Pinto E
    Frontiers in molecular neuroscience 6. (2013): 23.

    Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions.
    Longmore GD
    Molecular biology of the cell 11.10 (2000 Oct): 3299-313.

    c-Jun amino-terminal kinase is regulated by Galpha12/Galpha13 and obligate for differentiation of P19 embryonal carcinoma cells by retinoic acid.
    Malbon CC
    The Journal of biological chemistry 272.39 (1997 Sep 26): 24468-74.

    Formin proteins of the DAAM subfamily play a role during axon growth.
    Mihály J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.49 (2008 Dec 3): 13310-9.

    Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
    Ramalho-Santos M
    Nature 460.7257 (2009 Aug 13): 863-8.

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid.
    Strickland S
    Developmental biology 70.2 (1979 Jun): 515-21.

    Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm.
    Solter D
    Experimental cell research 137.2 (1982 Feb): 485-8.

    Distribution of murine stage-specific embryonic antigens in the kidneys of three rodent species.
    Dawjanov I
    Experimental cell research 140.2 (1982 Aug): 331-9.

    Monoclonal antibody against SSEA-1 is specific for a subpopulation of astrocytes in mouse cerebellum.
    Knowles B
    Neuroscience letters 31.2 (1982 Aug 16): 181-4.

    Episome-generated N-myc antisense RNA restricts the differentiation potential of primitive neuroectodermal cell lines.
    Neckers LM
    Molecular and cellular biology 11.3 (1991 Mar): 1360-71.

    Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
    Etches RJ
    Development (Cambridge, England) 122.8 (1996 Aug): 2339-48.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines.
    Smith AG
    Biology of reproduction 68.1 (2003 Jan): 222-9.

    Combined effects of Matrigel and growth factors on maintaining undifferentiated murine embryonic stem cells for embryotoxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 18.4 (2004 Aug): 543-53.

    Failure to express GAP-43 leads to disruption of a multipotent precursor and inhibits astrocyte differentiation.
    Meiri KF
    Molecular and cellular neurosciences 26.3 (2004 Jul): 390-405.

    Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 19.3 (2005 Apr): 389-97.

    Periderm prevents pathological epithelial adhesions during embryogenesis.
    Dixon MJ
    The Journal of clinical investigation 124.9 (2014 Sep): 3891-900.

    Efficient long-term cryopreservation of pluripotent stem cells at -80 °C.
    Han X
    Scientific reports 6. (2016 Oct 3): 34476.

    Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer's Disease.
    Soto C
    Cells 10.7 (2021 Jul 16): .

    SSEA-1, a stage-specific embryonic antigen of the mouse, is carried by the glycoprotein-bound large carbohydrate in embryonal carcinoma cells.
    Solter D
    Cell differentiation 16.3 (1985 May): 169-73.

    Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers.
    Wong CH
    Proceedings of the National Academy of Sciences of the United States of America 111.7 (2014 Feb 18): 2482-7.

    Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
    Rao MS
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 243-58.

    Long-term culture of human embryonic stem cells in feeder-free conditions.
    Carpenter MK
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 259-74.

    A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells.
    Jin J
    Nature chemical biology 7.8 (2011 Jul 10): 566-74.

    Pluripotency of mesenchymal stem cells derived from adult marrow.
    Verfaillie CM
    Nature 418.6893 (2002 Jul 4): 41-9.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
    Knowles BB
    Proceedings of the National Academy of Sciences of the United States of America 75.11 (1978 Nov): 5565-9.

    Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains.
    Evans MJ
    Nature 292.5819 (1981 Jul 9): 156-8.

    The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note.
    Knowles BB
    Biochemical and biophysical research communications 100.4 (1981 Jun): 1578-86.

    Tumor location detected with radioactively labeled monoclonal antibody and external scintigraphy.
    Solter D
    Science (New York, N.Y.) 206.4420 (1979 Nov 16): 844-7.

    Expression of H-2, laminin and SV40 T and TASA on differentiation of transformed murine teratocarcinoma cells.
    Huebner K
    Nature 288.5791 (1980 Dec 11): 615-8.

    Murine embryonic antigen (SSEA-1) is expressed on human cells and structurally related human blood group antigen I is expressed on mouse embryos.
    Solter D
    Developmental biology 93.1 (1982 Sep): 54-8.

    Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation.
    Ho HN
    Human reproduction (Oxford, England) 22.2 (2007 Feb): 567-77.

    Optimization of slow cooling cryopreservation for human pluripotent stem cells.
    Suemori H
    Genesis (New York, N.Y. : 2000) 52.1 (2014 Jan): 49-55.

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