MC-480 (SSEA-1)

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$40.00
SKU: MC-480 (SSEA-1)
View product citations for antibody MC-480 (SSEA-1) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Stage-specific embryonic antigen 1/CD15
Hybridoma Cells Available:
Antigen Species: Mouse
Depositor: Solter, D. / Knowles, B.B
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institute of Medical Biology, Singapore
Positive Tested Species Reactivity: Bovine, Chicken, Drosophila, Equine, Human, Mouse, Primate, Rat
Depositors Notes: MC-480 recognizes mouse embryos, mouse embryonic cells (EC), mouse embryonic stem cells (ES) and mouse & human embryonic germ cells (EG). MC-480 does not bind to human EC, ES or iPS cells.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Irradiated testis embryonal carcinoma F9 cell line
Alternate Gene Names:
Alternate Antibody Name: anti-CD15, anti-Lewis X
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-X63-Ag8
Epitope Location or Sequence: SSEA-1 is a carbohydrate epitope on glycolipids and glycoproteins involving fucosylated [GalBeta1-4(FucAlpha1-3)GlcNAcBeta1-R] type 2 blood group chains.
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528475 
Additional Information: For experiments involving live cells and tissue, MC-480 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions. MC-480 has been successfully used for TLC Overlay applications (PMID: 26405105).
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MC-480 (SSEA-1) was deposited to the DSHB by Solter, D. / Knowles, B.B (DSHB Hybridoma Product MC-480 (SSEA-1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

46 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
    Knowles BB
    Proceedings of the National Academy of Sciences of the United States of America 75.11 (1978 Nov): 5565-9.

    IF References

    Differential regulation of proliferation and neuronal differentiation in adult rat spinal cord neural stem/progenitors by ERK1/2, Akt, and PLCγ.
    Recio-Pinto E
    Frontiers in molecular neuroscience 6. (2013): 23.

    Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions.
    Longmore GD
    Molecular biology of the cell 11.10 (2000 Oct): 3299-313.

    c-Jun amino-terminal kinase is regulated by Galpha12/Galpha13 and obligate for differentiation of P19 embryonal carcinoma cells by retinoic acid.
    Malbon CC
    The Journal of biological chemistry 272.39 (1997 Sep 26): 24468-74.

    Formin proteins of the DAAM subfamily play a role during axon growth.
    Mihály J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.49 (2008 Dec 3): 13310-9.

    Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
    Ramalho-Santos M
    Nature 460.7257 (2009 Aug 13): 863-8.

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid.
    Strickland S
    Developmental biology 70.2 (1979 Jun): 515-21.

    Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm.
    Solter D
    Experimental cell research 137.2 (1982 Feb): 485-8.

    Distribution of murine stage-specific embryonic antigens in the kidneys of three rodent species.
    Dawjanov I
    Experimental cell research 140.2 (1982 Aug): 331-9.

    Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.
    Fraley EE
    The American journal of pathology 108.2 (1982 Aug): 225-30.

    Monoclonal antibody against SSEA-1 is specific for a subpopulation of astrocytes in mouse cerebellum.
    Knowles B
    Neuroscience letters 31.2 (1982 Aug 16): 181-4.

    Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.
    Solter D
    Cancer research 43.2 (1983 Feb): 669-78.

    Episome-generated N-myc antisense RNA restricts the differentiation potential of primitive neuroectodermal cell lines.
    Neckers LM
    Molecular and cellular biology 11.3 (1991 Mar): 1360-71.

    Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
    Etches RJ
    Development (Cambridge, England) 122.8 (1996 Aug): 2339-48.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines.
    Smith AG
    Biology of reproduction 68.1 (2003 Jan): 222-9.

    Combined effects of Matrigel and growth factors on maintaining undifferentiated murine embryonic stem cells for embryotoxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 18.4 (2004 Aug): 543-53.

    Failure to express GAP-43 leads to disruption of a multipotent precursor and inhibits astrocyte differentiation.
    Meiri KF
    Molecular and cellular neurosciences 26.3 (2004 Jul): 390-405.

    Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 19.3 (2005 Apr): 389-97.

    Periderm prevents pathological epithelial adhesions during embryogenesis.
    Dixon MJ
    The Journal of clinical investigation 124.9 (2014 Sep): 3891-900.

    Efficient long-term cryopreservation of pluripotent stem cells at -80 °C.
    Han X
    Scientific reports 6. (2016 Oct 3): 34476.

    WB References
    IHC References

    Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
    Imai H
    Genes to cells : devoted to molecular and cellular mechanisms 16.7 (2011 Jul): 815-25.

    Rostro-caudal gradual loss of cellular diversity within the periventricular regions of the ventricular system.
    Storch A
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 928-41.

    Derivation of pluripotent stem cells from cultured human primordial germ cells.
    Gearhart JD
    Proceedings of the National Academy of Sciences of the United States of America 95.23 (1998 Nov 10): 13726-31.

    Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues.
    Solter D
    Developmental biology 83.2 (1981 Apr 30): 391-8.

    Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.
    Fraley EE
    The American journal of pathology 108.2 (1982 Aug): 225-30.

    Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.
    Solter D
    Cancer research 43.2 (1983 Feb): 669-78.

    Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain.
    Spicer SS
    Glycoconjugate journal 13.3 (1996 Jun): 367-75.

    Nodal and bone morphogenetic protein 5 interact in murine mesoderm formation and implantation.
    Iannaccone PM
    Genesis (New York, N.Y. : 2000) 28.1 (2000 Sep): 1-14.

    The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.
    Calame KL
    Mechanisms of development 117.1-2 (2002 Sep): 305-9.

    Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells.
    Hanley NA
    Stem cells (Dayton, Ohio) 21.5 (2003): 598-609.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    IP References
    FFPE References

    Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
    Ramalho-Santos M
    Nature 460.7257 (2009 Aug 13): 863-8.

    Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers.
    Wong CH
    Proceedings of the National Academy of Sciences of the United States of America 111.7 (2014 Feb 18): 2482-7.

    FACS References

    Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
    Rao MS
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 243-58.

    Long-term culture of human embryonic stem cells in feeder-free conditions.
    Carpenter MK
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 259-74.

    A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells.
    Jin J
    Nature chemical biology 7.8 (2011 Jul 10): 566-74.

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    Pluripotency of mesenchymal stem cells derived from adult marrow.
    Verfaillie CM
    Nature 418.6893 (2002 Jul 4): 41-9.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Epitope Map References
    All References

    Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
    Imai H
    Genes to cells : devoted to molecular and cellular mechanisms 16.7 (2011 Jul): 815-25.

    Rostro-caudal gradual loss of cellular diversity within the periventricular regions of the ventricular system.
    Storch A
    Stem cells (Dayton, Ohio) 27.4 (2009 Apr): 928-41.

    Derivation of pluripotent stem cells from cultured human primordial germ cells.
    Gearhart JD
    Proceedings of the National Academy of Sciences of the United States of America 95.23 (1998 Nov 10): 13726-31.

    Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues.
    Solter D
    Developmental biology 83.2 (1981 Apr 30): 391-8.

    Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.
    Fraley EE
    The American journal of pathology 108.2 (1982 Aug): 225-30.

    Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.
    Solter D
    Cancer research 43.2 (1983 Feb): 669-78.

    Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain.
    Spicer SS
    Glycoconjugate journal 13.3 (1996 Jun): 367-75.

    Nodal and bone morphogenetic protein 5 interact in murine mesoderm formation and implantation.
    Iannaccone PM
    Genesis (New York, N.Y. : 2000) 28.1 (2000 Sep): 1-14.

    The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.
    Calame KL
    Mechanisms of development 117.1-2 (2002 Sep): 305-9.

    Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells.
    Hanley NA
    Stem cells (Dayton, Ohio) 21.5 (2003): 598-609.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Differential regulation of proliferation and neuronal differentiation in adult rat spinal cord neural stem/progenitors by ERK1/2, Akt, and PLCγ.
    Recio-Pinto E
    Frontiers in molecular neuroscience 6. (2013): 23.

    Ajuba, a cytosolic LIM protein, shuttles into the nucleus and affects embryonal cell proliferation and fate decisions.
    Longmore GD
    Molecular biology of the cell 11.10 (2000 Oct): 3299-313.

    c-Jun amino-terminal kinase is regulated by Galpha12/Galpha13 and obligate for differentiation of P19 embryonal carcinoma cells by retinoic acid.
    Malbon CC
    The Journal of biological chemistry 272.39 (1997 Sep 26): 24468-74.

    Formin proteins of the DAAM subfamily play a role during axon growth.
    Mihály J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.49 (2008 Dec 3): 13310-9.

    Chd1 regulates open chromatin and pluripotency of embryonic stem cells.
    Ramalho-Santos M
    Nature 460.7257 (2009 Aug 13): 863-8.

    Different expression levels of alpha3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines.
    Costa J
    International journal of oncology 29.3 (2006 Sep): 557-66.

    The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid.
    Strickland S
    Developmental biology 70.2 (1979 Jun): 515-21.

    Teratocarcinoma antigen is secreted by epididymal cells and coupled to maturing sperm.
    Solter D
    Experimental cell research 137.2 (1982 Feb): 485-8.

    Distribution of murine stage-specific embryonic antigens in the kidneys of three rodent species.
    Dawjanov I
    Experimental cell research 140.2 (1982 Aug): 331-9.

    Monoclonal antibody against SSEA-1 is specific for a subpopulation of astrocytes in mouse cerebellum.
    Knowles B
    Neuroscience letters 31.2 (1982 Aug 16): 181-4.

    Episome-generated N-myc antisense RNA restricts the differentiation potential of primitive neuroectodermal cell lines.
    Neckers LM
    Molecular and cellular biology 11.3 (1991 Mar): 1360-71.

    Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities.
    Etches RJ
    Development (Cambridge, England) 122.8 (1996 Aug): 2339-48.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines.
    Smith AG
    Biology of reproduction 68.1 (2003 Jan): 222-9.

    Combined effects of Matrigel and growth factors on maintaining undifferentiated murine embryonic stem cells for embryotoxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 18.4 (2004 Aug): 543-53.

    Failure to express GAP-43 leads to disruption of a multipotent precursor and inhibits astrocyte differentiation.
    Meiri KF
    Molecular and cellular neurosciences 26.3 (2004 Jul): 390-405.

    Comparison of Matrigel and gelatin substrata for feeder-free culture of undifferentiated mouse embryonic stem cells for toxicity testing.
    Liu K
    Toxicology in vitro : an international journal published in association with BIBRA 19.3 (2005 Apr): 389-97.

    Periderm prevents pathological epithelial adhesions during embryogenesis.
    Dixon MJ
    The Journal of clinical investigation 124.9 (2014 Sep): 3891-900.

    Efficient long-term cryopreservation of pluripotent stem cells at -80 °C.
    Han X
    Scientific reports 6. (2016 Oct 3): 34476.

    SSEA-1, a stage-specific embryonic antigen of the mouse, is carried by the glycoprotein-bound large carbohydrate in embryonal carcinoma cells.
    Solter D
    Cell differentiation 16.3 (1985 May): 169-73.

    Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers.
    Wong CH
    Proceedings of the National Academy of Sciences of the United States of America 111.7 (2014 Feb 18): 2482-7.

    Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
    Rao MS
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 243-58.

    Long-term culture of human embryonic stem cells in feeder-free conditions.
    Carpenter MK
    Developmental dynamics : an official publication of the American Association of Anatomists 229.2 (2004 Feb): 259-74.

    A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells.
    Jin J
    Nature chemical biology 7.8 (2011 Jul 10): 566-74.

    Pluripotency of mesenchymal stem cells derived from adult marrow.
    Verfaillie CM
    Nature 418.6893 (2002 Jul 4): 41-9.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
    Knowles BB
    Proceedings of the National Academy of Sciences of the United States of America 75.11 (1978 Nov): 5565-9.

    Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains.
    Evans MJ
    Nature 292.5819 (1981 Jul 9): 156-8.

    The hapten structure of a developmentally regulated glycolipid antigen (SSEA-1) isolated from human erythrocytes and adenocarcinoma: a preliminary note.
    Knowles BB
    Biochemical and biophysical research communications 100.4 (1981 Jun): 1578-86.

    Tumor location detected with radioactively labeled monoclonal antibody and external scintigraphy.
    Solter D
    Science (New York, N.Y.) 206.4420 (1979 Nov 16): 844-7.

    Expression of H-2, laminin and SV40 T and TASA on differentiation of transformed murine teratocarcinoma cells.
    Huebner K
    Nature 288.5791 (1980 Dec 11): 615-8.

    Murine embryonic antigen (SSEA-1) is expressed on human cells and structurally related human blood group antigen I is expressed on mouse embryos.
    Solter D
    Developmental biology 93.1 (1982 Sep): 54-8.

    Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation.
    Ho HN
    Human reproduction (Oxford, England) 22.2 (2007 Feb): 567-77.

    Optimization of slow cooling cryopreservation for human pluripotent stem cells.
    Suemori H
    Genesis (New York, N.Y. : 2000) 52.1 (2014 Jan): 49-55.

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