TROMA-I

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$40.00
SKU: TROMA-I
View product citations for antibody TROMA-I on CiteAb

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Available: 443

DSHB Data Sheet

Catalog Fields

Antigen: Keratin, type II/ Cytokeratin 8
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Brulet, P. / Kemler, R.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Institut Pasteur
Positive Tested Species Reactivity: Canine, Human, Mouse
Depositors Notes: The TROMA-I antibody is also known as EndoA.
Antigen Molecular Weight: Predicted: 54.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Krt8
Immunogen: TDM1 (Trophoblastoma) cell line intermediate filaments
Alternate Gene Names: krt2-8
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: P11679 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16691 
Additional Characterization:  
Antibody Registry ID: AB_531826 
Additional Information: works in paraffin; recognizes keratin 8;
Recommended Applications: FACS, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TROMA-I was deposited to the DSHB by Brulet, P. / Kemler, R. (DSHB Hybridoma Product TROMA-I)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

41 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • FACS References
  • All References
  • Initial Publication

    Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.
    Jacob F
    Proceedings of the National Academy of Sciences of the United States of America 77.7 (1980 Jul): 4113-7.

    IF References

    Lineage specific composition of cyclin D-CDK4/CDK6-p27 complexes reveals distinct functions of CDK4, CDK6 and individual D-type cyclins in differentiating cells of embryonic origin.
    Hampl A
    Cell proliferation 41.6 (2008 Dec): 875-893.

    Abnormal development of mouse embryoid bodies lacking p27Kip1 cell cycle regulator.
    Hampl A
    Stem cells (Dayton, Ohio) 23.7 (2005 Aug): 965-74.

    Differential BMP signaling controls formation and differentiation of multipotent preplacodal ectoderm progenitors from human embryonic stem cells.
    Li JY
    Developmental biology 379.2 (2013 Jul 15): 208-20.

    Reactivity of monoclonal antibodies against intermediate filament proteins during embryonic development.
    Jacob F
    Journal of embryology and experimental morphology 64. (1981 Aug): 45-60.

    A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.
    Prunieras M
    Experimental cell research 154.1 (1984 Sep): 315-9.

    Immortalised mouse submandibular epithelial cell lines retain polarised structural and functional properties.
    Rougeon F
    Journal of cell science 109 ( Pt 12). (1996 Dec): 2789-800.

    microRNA-449a modulates medullary thymic epithelial cell differentiation.
    Zhang X
    Scientific reports 7.1 (2017 Nov 21): 15915.

    Insulin-Like Growth Factors Are Expressed in the Taste System, but Do Not Maintain Adult Taste Buds.
    Krimm RF
    PloS one 11.2 (2016): e0148315.

    Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement.
    Knox SM
    EMBO molecular medicine 10.3 (2018 Mar): .

    Development of Full Sweet, Umami, and Bitter Taste Responsiveness Requires Regulator of G protein Signaling-21 (RGS21).
    Setola V
    Chemical senses 43.5 (2018 May 23): 367-378.

    Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5.
    Liu Y
    Cell reports 15.5 (2016 May 3): 1111-1122.

    Sustained Endocannabinoid Signaling Compromises Decidual Function and Promotes Inflammation-induced Preterm Birth.
    Dey SK
    The Journal of biological chemistry 291.15 (2016 Apr 8): 8231-40.

    Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib.
    Mistretta CM
    Scientific reports 8.1 (2018 Nov 1): 16150.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    Coordinated hedgehog signaling induces new hair follicles in adult skin.
    Kasper M
    eLife 9. (2020 Mar 17): .

    Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.
    Schiller HB
    Nature communications 11.1 (2020 Jul 16): 3559.

    Three-dimensional Organization of Layered Apical Cytoskeletal Networks Associated with Mouse Airway Tissue Development.
    Tsukita S
    Scientific reports 7. (2017 Mar 8): 43783.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    LEFTY1 Is a Dual-SMAD Inhibitor that Promotes Mammary Progenitor Growth and Tumorigenesis.
    Clarke MF
    Cell stem cell 27.2 (2020 Aug 6): 284-299.e8.

    RSK2 Maintains Adult Estrogen Homeostasis by Inhibiting ERK1/2-Mediated Degradation of Estrogen Receptor Alpha.
    Lannigan DA
    Cell reports 32.3 (2020 Jul 21): 107931.

    Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures.
    Izpisua Belmonte JC
    Cell 179.3 (2019 Oct 17): 687-702.e18.

    WB References

    Abnormal development of mouse embryoid bodies lacking p27Kip1 cell cycle regulator.
    Hampl A
    Stem cells (Dayton, Ohio) 23.7 (2005 Aug): 965-74.

    Genome-wide reduction in H3K9 acetylation during human embryonic stem cell differentiation.
    Bártová E
    Journal of cellular physiology 219.3 (2009 Jun): 677-87.

    Epigenome and chromatin structure in human embryonic stem cells undergoing differentiation.
    Kozubek S
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3690-702.

    Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid: inhibitory effect of serum.
    Hampl A
    Physiological research 54.1 (2005): 115-22.

    Increased apoptosis in differentiating p27-deficient mouse embryonic stem cells.
    Hampl A
    Cellular and molecular life sciences : CMLS 61.11 (2004 Jun): 1384-400.

    A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.
    Prunieras M
    Experimental cell research 154.1 (1984 Sep): 315-9.

    PTEN dephosphorylates Abi1 to promote epithelial morphogenesis.
    Li S
    The Journal of cell biology 219.9 (2020 Sep 7): .

    IHC References

    Intrinsic cues and hormones control mouse mammary epithelial tree size.
    Garcia-Sanz JA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.9 (2012 Sep): 3844-53.

    Optimization of the immunohistochemical demonstration of keratins in paraffin wax-embedded mouse skin.
    García-Iglesias MJ
    Journal of comparative pathology 119.2 (1998 Aug): 177-81.

    Neural crest origin of mammalian Merkel cells.
    Sieber-Blum M
    Developmental biology 253.2 (2003 Jan 15): 258-63.

    Reduced metastasis of transgenic mammary cancer in urokinase-deficient mice.
    Johnsen M
    International journal of cancer 113.4 (2005 Feb 10): 525-32.

    Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells.
    Clarke MF
    Cell 138.3 (2009 Aug 7): 592-603.

    Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian axis leads to ovarian epithelial tumorigenesis in mice.
    Bagchi IC
    PLoS genetics 10.3 (2014 Mar): e1004230.

    PI3K/AKT pathway regulates phosphorylation of steroid receptors, hormone independence and tumor differentiation in breast cancer.
    Novaro V
    Carcinogenesis 33.3 (2012 Mar): 509-18.

    A method of producing genetically manipulated mouse mammary gland.
    Semba K
    Breast cancer research : BCR 21.1 (2019 Jan 5): 1.

    ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion.
    Chandler RL
    Nature communications 10.1 (2019 Aug 7): 3554.

    Self-organized formation of developing appendages from murine pluripotent stem cells.
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    A mouse model of endometriosis mimicking the natural spread of invasive endometrium.
    Chandler RL
    Human reproduction (Oxford, England) 35.1 (2020 Jan 1): 58-69.

    Distinct Spatiotemporally Dynamic Wnt-Secreting Niches Regulate Proximal Airway Regeneration and Aging.
    Gomperts BN
    Cell stem cell 27.3 (2020 Sep 3): 413-429.e4.

    A trans fatty acid substitute enhanced development of liver proliferative lesions induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet.
    Nakae D
    Lipids in health and disease 19.1 (2020 Dec 14): 251.

    FFPE References
    FACS References
    All References

    Intrinsic cues and hormones control mouse mammary epithelial tree size.
    Garcia-Sanz JA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.9 (2012 Sep): 3844-53.

    Optimization of the immunohistochemical demonstration of keratins in paraffin wax-embedded mouse skin.
    García-Iglesias MJ
    Journal of comparative pathology 119.2 (1998 Aug): 177-81.

    Neural crest origin of mammalian Merkel cells.
    Sieber-Blum M
    Developmental biology 253.2 (2003 Jan 15): 258-63.

    Reduced metastasis of transgenic mammary cancer in urokinase-deficient mice.
    Johnsen M
    International journal of cancer 113.4 (2005 Feb 10): 525-32.

    Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells.
    Clarke MF
    Cell 138.3 (2009 Aug 7): 592-603.

    Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian axis leads to ovarian epithelial tumorigenesis in mice.
    Bagchi IC
    PLoS genetics 10.3 (2014 Mar): e1004230.

    PI3K/AKT pathway regulates phosphorylation of steroid receptors, hormone independence and tumor differentiation in breast cancer.
    Novaro V
    Carcinogenesis 33.3 (2012 Mar): 509-18.

    A method of producing genetically manipulated mouse mammary gland.
    Semba K
    Breast cancer research : BCR 21.1 (2019 Jan 5): 1.

    ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion.
    Chandler RL
    Nature communications 10.1 (2019 Aug 7): 3554.

    Self-organized formation of developing appendages from murine pluripotent stem cells.
    Eiraku M
    Nature communications 10.1 (2019 Aug 23): 3802.

    A mouse model of endometriosis mimicking the natural spread of invasive endometrium.
    Chandler RL
    Human reproduction (Oxford, England) 35.1 (2020 Jan 1): 58-69.

    Distinct Spatiotemporally Dynamic Wnt-Secreting Niches Regulate Proximal Airway Regeneration and Aging.
    Gomperts BN
    Cell stem cell 27.3 (2020 Sep 3): 413-429.e4.

    A trans fatty acid substitute enhanced development of liver proliferative lesions induced in mice by feeding a choline-deficient, methionine-lowered, L-amino acid-defined, high-fat diet.
    Nakae D
    Lipids in health and disease 19.1 (2020 Dec 14): 251.

    Lineage specific composition of cyclin D-CDK4/CDK6-p27 complexes reveals distinct functions of CDK4, CDK6 and individual D-type cyclins in differentiating cells of embryonic origin.
    Hampl A
    Cell proliferation 41.6 (2008 Dec): 875-893.

    Abnormal development of mouse embryoid bodies lacking p27Kip1 cell cycle regulator.
    Hampl A
    Stem cells (Dayton, Ohio) 23.7 (2005 Aug): 965-74.

    Differential BMP signaling controls formation and differentiation of multipotent preplacodal ectoderm progenitors from human embryonic stem cells.
    Li JY
    Developmental biology 379.2 (2013 Jul 15): 208-20.

    Reactivity of monoclonal antibodies against intermediate filament proteins during embryonic development.
    Jacob F
    Journal of embryology and experimental morphology 64. (1981 Aug): 45-60.

    A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.
    Prunieras M
    Experimental cell research 154.1 (1984 Sep): 315-9.

    Immortalised mouse submandibular epithelial cell lines retain polarised structural and functional properties.
    Rougeon F
    Journal of cell science 109 ( Pt 12). (1996 Dec): 2789-800.

    microRNA-449a modulates medullary thymic epithelial cell differentiation.
    Zhang X
    Scientific reports 7.1 (2017 Nov 21): 15915.

    Insulin-Like Growth Factors Are Expressed in the Taste System, but Do Not Maintain Adult Taste Buds.
    Krimm RF
    PloS one 11.2 (2016): e0148315.

    Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement.
    Knox SM
    EMBO molecular medicine 10.3 (2018 Mar): .

    Development of Full Sweet, Umami, and Bitter Taste Responsiveness Requires Regulator of G protein Signaling-21 (RGS21).
    Setola V
    Chemical senses 43.5 (2018 May 23): 367-378.

    Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5.
    Liu Y
    Cell reports 15.5 (2016 May 3): 1111-1122.

    Sustained Endocannabinoid Signaling Compromises Decidual Function and Promotes Inflammation-induced Preterm Birth.
    Dey SK
    The Journal of biological chemistry 291.15 (2016 Apr 8): 8231-40.

    Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib.
    Mistretta CM
    Scientific reports 8.1 (2018 Nov 1): 16150.

    Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
    Xia Y
    Cell stem cell 25.3 (2019 Sep 5): 373-387.e9.

    Coordinated hedgehog signaling induces new hair follicles in adult skin.
    Kasper M
    eLife 9. (2020 Mar 17): .

    Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.
    Schiller HB
    Nature communications 11.1 (2020 Jul 16): 3559.

    Three-dimensional Organization of Layered Apical Cytoskeletal Networks Associated with Mouse Airway Tissue Development.
    Tsukita S
    Scientific reports 7. (2017 Mar 8): 43783.

    LEFTY1 Is a Dual-SMAD Inhibitor that Promotes Mammary Progenitor Growth and Tumorigenesis.
    Clarke MF
    Cell stem cell 27.2 (2020 Aug 6): 284-299.e8.

    RSK2 Maintains Adult Estrogen Homeostasis by Inhibiting ERK1/2-Mediated Degradation of Estrogen Receptor Alpha.
    Lannigan DA
    Cell reports 32.3 (2020 Jul 21): 107931.

    Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures.
    Izpisua Belmonte JC
    Cell 179.3 (2019 Oct 17): 687-702.e18.

    Genome-wide reduction in H3K9 acetylation during human embryonic stem cell differentiation.
    Bártová E
    Journal of cellular physiology 219.3 (2009 Jun): 677-87.

    Epigenome and chromatin structure in human embryonic stem cells undergoing differentiation.
    Kozubek S
    Developmental dynamics : an official publication of the American Association of Anatomists 237.12 (2008 Dec): 3690-702.

    Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid: inhibitory effect of serum.
    Hampl A
    Physiological research 54.1 (2005): 115-22.

    Increased apoptosis in differentiating p27-deficient mouse embryonic stem cells.
    Hampl A
    Cellular and molecular life sciences : CMLS 61.11 (2004 Jun): 1384-400.

    PTEN dephosphorylates Abi1 to promote epithelial morphogenesis.
    Li S
    The Journal of cell biology 219.9 (2020 Sep 7): .

    Regeneration of the adult thymus is preceded by the expansion of K5+K8+ epithelial cell progenitors and by increased expression of Trp63, cMyc and Tcf3 transcription factors in the thymic stroma.
    Zaitseva M
    International immunology 19.11 (2007 Nov): 1249-60.

    Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.
    Jacob F
    Proceedings of the National Academy of Sciences of the United States of America 77.7 (1980 Jul): 4113-7.

    Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia.
    Souza RF
    The Journal of clinical investigation 124.9 (2014 Sep): 3767-80.

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