Antibody Name: TROMA-I
Antigen:Keratin, type II/ Cytokeratin 8
Immunofluorescent image of the placenta of a pregnant mouse labeled with DSHB antibody TROMA-I against Cytokeratin 8 (red). Antigen retrieval pH6 citrate buffer. Image Credit: Kannan Athilakshmi.
Catalog Fields
Identity
Clone ID / Antibody Name: TROMA-I
Alternate Antibody Name: —
Antigen Name: Keratin, type II/ Cytokeratin 8
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: Krt8
Entrez Gene ID: 16691
Antigen Sequence: —
Antigen Species: Mouse
Antigen Mol. Weight: Predicted: 54.5 kDa
Uniprot ID: P11679
Human Protein Atlas: —
Antibody Properties
Host Species: Rat
Clonality: Monoclonal
Isotype: Rat IgG2a, kappa light chain
Myeloma Strain: SP2/0
Antibody Registry / RRID: AB_531826
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: TDM1 (Trophoblastoma) cell line intermediate filaments
Immunogen Sequence: Full length protein
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: TROMA-I specificity for keratin K8 has been knock-out validated. See PMID: 27234655.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Canine, Human, Mouse
Predicted Reactivity: —
Depositor
Depositor Name: P. Brulet / R. Kemler
Institution: Institut Pasteur
Deposit Date: February 13, 1987
Depositor Notes: The TROMA-I antibody is also known as EndoA.
Recommended Applications
FACS
FFPE
IF
IHC
IP
WB
Each lab must optimize concentration per application and species. Full usage recommendations →
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Could not load UniProt data for P11679



![Immunofluorescent (IF) image, acquired with a fluorescence microscope, of mammary tissue sections from mice labeled with DSHB anti-Krt8 [TROMA-I] (in red). PMID: 39321020, Fig. 2I](https://dshb.biology.uiowa.edu/SSP Applications/NetSuite Inc. - SCS/SuiteCommerce Standard/img/TROMA-I_media-02.png?resizeid=3&resizeh=600&resizew=600)
![Immunofluorescent (IF) image, acquired with a fluorescence microscope, of mammary tissue sections from mice labeled with DSHB anti-Krt8 [TROMA-I] (in red). PMID: 39321020, Fig. 3F](https://dshb.biology.uiowa.edu/SSP Applications/NetSuite Inc. - SCS/SuiteCommerce Standard/img/TROMA-I_media-03.png?resizeid=3&resizeh=600&resizew=600)