MH27
Catalog Fields
Antigen: Apical junction molecule-1 (cell-junction marker ajm-1)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Waterston, R.H.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Washington
Positive Tested Species Reactivity: C. elegans
Depositors Notes: MH27 stains cell-cell junctions in hypodermis, pharynx, and gut. See Nematology May 2021 for immunostaining fixation protocols.
Predicted Species Reactivity:
Gene: ajm-1
Immunogen: Crude protein fraction
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/O-ag
Epitope Location or Sequence:
Uniprot ID: A0A1C3NSP2
Immunogen Sequence: Full length protein
Entrez Gene ID: 181148
Antibody Registry ID: AB_531819
Additional Information: There is no immunoreactivity in mutant embryos. There are multiple Uniprot entries for this protein; A0A1C3NSP2 is the ID for the largast isoform that cantains all exon products.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MH27 was deposited to the DSHB by Waterston, R.H. (DSHB Hybridoma Product MH27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Muscle cell attachment in Caenorhabditis elegans.
Waterston RH
The Journal of cell biology 114.3 (1991 Aug): 465-79.