MH27

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$40.00
SKU: MH27
View product citations for antibody MH27 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Apical junction molecule-1 (cell-junction marker ajm-1)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Waterston, R.H.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Washington
Positive Tested Species Reactivity: C. elegans
Depositors Notes: MH27 stains cell-cell junctions in hypodermis, pharynx, and gut.
Antigen Molecular Weight: Multiple isoforms; Predicted: from 142 to 222 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ajm-1
Immunogen: Crude protein fraction
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/O-ag
Epitope Location or Sequence:
Uniprot ID: A0A1C3NSP2 
Immunogen Sequence: Full length protein
Entrez Gene ID: 181148 
Additional Characterization:  
Antibody Registry ID: AB_531819 
Additional Information: There is no immunoreactivity in mutant embryos. There are multiple Uniprot entries for this protein; A0A1C3NSP2 is the ID for the largast isoform that cantains all exon products.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MH27 was deposited to the DSHB by Waterston, R.H. (DSHB Hybridoma Product MH27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Muscle cell attachment in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 114.3 (1991 Aug): 465-79.

    IF References

    Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia.
    Hardin JD
    Nature cell biology 3.11 (2001 Nov): 983-91.

    Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes.
    Kitagawa H
    The Journal of biological chemistry 291.44 (2016 Oct 28): 23294-23304.

    Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation.
    Askjaer P
    PLoS genetics 12.6 (2016 Jun): e1006131.

    CDC-42 Orients Cell Migration during Epithelial Intercalation in the Caenorhabditis elegans Epidermis.
    Hardin J
    PLoS genetics 12.11 (2016 Nov): e1006415.

    Morphogenesis of the C. elegans Intestine Involves Axon Guidance Genes.
    Priess JR
    PLoS genetics 12.4 (2016 Apr): e1005950.

    LET-413 is a basolateral protein required for the assembly of adherens junctions in Caenorhabditis elegans.
    Labouesse M
    Nature cell biology 2.7 (2000 Jul): 415-22.

    The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis.
    Maina CV
    Developmental biology 313.2 (2008 Jan 15): 767-86.

    A gp330/megalin-related protein is required in the major epidermis of Caenorhabditis elegans for completion of molting.
    Han M
    Development (Cambridge, England) 126.3 (1999 Feb): 597-606.

    Genes critical for muscle development and function in Caenorhabditis elegans identified through lethal mutations.
    Waterston RH
    The Journal of cell biology 124.4 (1994 Feb): 475-90.

    Assembly of body wall muscle and muscle cell attachment structures in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 124.4 (1994 Feb): 491-506.

    Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 146.3 (1999 Aug 9): 659-72.

    The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex.
    Kipreos ET
    Molecular and cellular biology 27.4 (2007 Feb): 1394-406.

    WB References

    Muscle cell attachment in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 114.3 (1991 Aug): 465-79.

    All References

    Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia.
    Hardin JD
    Nature cell biology 3.11 (2001 Nov): 983-91.

    Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes.
    Kitagawa H
    The Journal of biological chemistry 291.44 (2016 Oct 28): 23294-23304.

    Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation.
    Askjaer P
    PLoS genetics 12.6 (2016 Jun): e1006131.

    CDC-42 Orients Cell Migration during Epithelial Intercalation in the Caenorhabditis elegans Epidermis.
    Hardin J
    PLoS genetics 12.11 (2016 Nov): e1006415.

    Morphogenesis of the C. elegans Intestine Involves Axon Guidance Genes.
    Priess JR
    PLoS genetics 12.4 (2016 Apr): e1005950.

    LET-413 is a basolateral protein required for the assembly of adherens junctions in Caenorhabditis elegans.
    Labouesse M
    Nature cell biology 2.7 (2000 Jul): 415-22.

    The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis.
    Maina CV
    Developmental biology 313.2 (2008 Jan 15): 767-86.

    A gp330/megalin-related protein is required in the major epidermis of Caenorhabditis elegans for completion of molting.
    Han M
    Development (Cambridge, England) 126.3 (1999 Feb): 597-606.

    Genes critical for muscle development and function in Caenorhabditis elegans identified through lethal mutations.
    Waterston RH
    The Journal of cell biology 124.4 (1994 Feb): 475-90.

    Assembly of body wall muscle and muscle cell attachment structures in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 124.4 (1994 Feb): 491-506.

    Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 146.3 (1999 Aug 9): 659-72.

    The Caenorhabditis elegans replication licensing factor CDT-1 is targeted for degradation by the CUL-4/DDB-1 complex.
    Kipreos ET
    Molecular and cellular biology 27.4 (2007 Feb): 1394-406.

    Muscle cell attachment in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 114.3 (1991 Aug): 465-79.

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