MH27
Catalog Fields
Antigen: Apical junction molecule-1 (cell-junction marker ajm-1)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Waterston, R.H.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Washington
Positive Tested Species Reactivity: C. elegans
Depositors Notes: MH27 stains cell-cell junctions in hypodermis, pharynx, and gut.
Antigen Molecular Weight: Multiple isoforms; Predicted: from 142 to 222 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: ajm-1
Immunogen: Crude protein fraction
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2/O-ag
Epitope Location or Sequence:
Uniprot ID: A0A1C3NSP2
Immunogen Sequence: Full length protein
Entrez Gene ID: 181148
Additional Characterization:
Antibody Registry ID: AB_531819
Additional Information: There is no immunoreactivity in mutant embryos. There are multiple Uniprot entries for this protein; A0A1C3NSP2 is the ID for the largast isoform that cantains all exon products.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MH27 was deposited to the DSHB by Waterston, R.H. (DSHB Hybridoma Product MH27)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Muscle cell attachment in Caenorhabditis elegans.
Waterston RH
The Journal of cell biology 114.3 (1991 Aug): 465-79.