DLG1
Catalog Fields
Antigen: DLG-1 (Discs Large)
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans
Depositors Institution: Washington University Medical School
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Depositors Notes: Works well on C. elegans whole mounts.
Immunogen: Recombinant His6-tagged DLG-1 (aa 204-593) fusion protein
Gene: dlg-1
Alternate Antibody Name: 5E2
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Uniprot ID: G5ECY0
Epitope Location or Sequence: aa 204-593, PDZ domains 1 to 3
Entrez Gene ID: 180819
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_2617529
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: This antigen is the C. elegans homolog of the Drosophila membrane-associated guanylate kinase (MAGUK) family of scaffolding protein discs large protein, after which DLG-1 is named. In C. elegans, DLG-1 is primarily associated with the adherens junctions.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DLG1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product DLG1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
7 References
Initial Publication