DLG1

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$40.00
SKU: DLG1
View product citations for antibody DLG1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: DLG-1 (Discs Large)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Depositors Institution: Washington University Medical School
Host Species: mouse
Depositors Notes: Works well on C. elegans whole mounts.
Positive Tested Species Reactivity: C. elegans
Gene: dlg-1
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged DLG-1 (aa 204-593) fusion protein
Epitope Mapped: Yes
Alternate Antibody Name: 5E2
Epitope Location or Sequence: aa 204-593, PDZ domains 1 to 3
Myeloma Strain: P3X63Ag8.653
Immunogen Sequence: Partial protein
Uniprot ID: G5ECY0 
Additional Information: This antigen is the C. elegans homolog of the Drosophila membrane-associated guanylate kinase (MAGUK) family of scaffolding protein discs large protein, after which DLG-1 is named. In C. elegans, DLG-1 is primarily associated with the adherens junctions.
Entrez Gene ID: 180819 
Antibody Registry ID: AB_2617529 
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DLG1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product DLG1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis.
    Gobel V
    Development (Cambridge, England) 139.11 (2012 Jun): 2071-83.

    WB References
    IHC References
    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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